Validation of Biomarkers of Diabetic Nephropathy in Type I Diabetic Children (peeDIAB2)

Identification and First Validation of Early Non-invasive Biomarkers of Diabetic Nephropathy in Type I Diabetic Children

About 30 to 40% of patients suffering from type I diabetes are at risk of developing a diabetic nephropathy (DN) leading more or less rapidly to an end-stage renal disease. Nowadays, the microalbuminuria is the most often used clinical parameter for possible onset of DN. However, it is a late (because it permits to detect a renal disease already present), non-specific and low sensitive biomarker.

Therefore the main objective of this study is to identify early urinary biomarkers predictive of DN in children with type I diabetes, before the appearance of a microalbuminuria.

Study Overview

• Status: Completed
• Conditions: Type 1 Diabetes Mellitus With Diabetic Nephropathy
• Intervention / Treatment: Other: Urinary sample collection

Detailed Description

Once the type I diabetes is diagnosed, the speed of progression to a DN is very variable. Thus, the discovery of biomarkers able to distinguish progressor patients from non-progressor since the appearance of a microalbuminuria, is crucial in order to take care of progressor patients the earlier and to slow down the disease progression.

The investigators have shown that urine is a biological sample extremely well suited for proteome analysis with the aim to identify biomarkers of renal damages. Indeed, they were the first to analyze the urinary proteome of infant using capillary electrophoresis-coupled mass spectrometry. This technic permits to analyse the entire urinary proteome of a person in one hour.

Several laboratories tried to identify other predictive urinary biomarkers of the development of a DN in diabetic patients, but with limited success and nowadays in the clinical practice they still use the measure of the microalbuminuria. Contrary to precedent studies, the investigators develop an analysis without a priori and starting with early samples (without any signs of DN) associating the urinary peptide profile with the DN progression over 9-10 years.

This is a nested case-control study in a cohort of 317 patients constituting the urinary biological collection. (The urinary samples of a type I diabetic cohort of 317 patients seen between 2004 and 2008 have been collected.) These patients will be contacted at the end of 2012 to obtain a number of participation of 180 patients authorizing the use aposteriori of their urinary samples. Among this cohort, the investigators evaluate at 90 the number of patients with a DN defined by a dosage of microalbuminuria higher than 100 mg/L.

The primary endpoint of this study is therefore the identification of urinary biomarkers predictive of DN in type I diabetes pediatric patients.

The secondary endpoints are :

• The estimation of sensibility and specificity parameters, and the Area Under the Curve (AUC).
• The identification of factors associated with the variability of biomarkers expression such as the age at the moment of the diagnosis, the time between the diagnosis and the urinary collection, the rate of microalbuminuria, and the type of insulin used by the patient.
• And in the subgroup of patients for whom two urinary samples have been collected, the investigators want to study the urinary proteome stability in patients with no DN in 2013.

• Study Type: Observational
• Enrollment (Actual): 150

Contacts and Locations

Study Locations

• France
  • Toulouse, France, 31059
    • Purpan Children Hospital
  • Toulouse, France, 31059
    • Rangueil hospital

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 6 years to 30 years (Child, Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Non-Probability Sample

Study Population

Patients are selected in a cohort of 317 type I diabetic children constituting a urine samples collection. Indeed, urine sample of 317 patients have been collected between 2004 and 2008. These patients will be called to obtain the participation of 180 children in this study.

Description

Inclusion Criteria:

• Patient with a type I diabetes diagnosed before 15 years-old
• Urinary collection done 5 years after the diagnosis
• Urinary collection done without any acute intermittent pathology
• Urinary collection done without any treatment (other than diabetes treatment)
• Glomerular filtration rate ≥ 60 mL/min at the moment of the urinary collection already done
• Informed consent obtained aposteriori for the analysis of the urinary samples collected between 2004 and 2008, and consent obtained for the analysis of urinary sample collected in 2013

Exclusion Criteria:

• Patients suffering from an autoimmune disease associated with the diabetes (vitiligo, Grave's disease, thyroiditis, pernicious anemia or Biermer's disease)
• Patient with a renal disease (other than diabetic nephropathy) at the first urinary collection (between 2004 and 2008)
• Pregnancy, because the urinary proteome and the microalbumin dosage can be modified during a pregnancy.
• Patient refusal to use urinary samples already collected

Study Plan

How is the study designed?

Design Details

• Observational Models: Cohort
• Time Perspectives: Prospective

Number of groups / cohorts

2

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
Progressor (ND); Group of patients with an albuminuria > 100mg/L, by Urinary sample collection	Other: Urinary sample collection; urinary sample collection will be done at the inclusion visit for the assessment of the microalbuminuria ( < or > than 100mg/L)
Non-Progressor (non-ND); Group of patients without an albuminuria > 100mg/L, by Urinary sample collection	Other: Urinary sample collection; urinary sample collection will be done at the inclusion visit for the assessment of the microalbuminuria ( < or > than 100mg/L)

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Identify predictive non-invasive urinary biomarkers	Urinary samples collection Assessment of the microalbuminuria to diagnose the diabetic nephropathy (DN)	Baseline (Inclusion day)

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Relation of urinary markers with patient characteristics	Patients characteristics : age of the patients at the diabetes diagnosis, their age at the urinary collection, their sex, the time between the diagnosis and the urinary collection, the type of insulin used.	Baseline (Inclusion day)

Collaborators and Investigators

• Sponsor: University Hospital, Toulouse
• Collaborators: Laboratory Inserm U858 Team 5 - RF-lab
• Investigators: Principal Investigator: Stephane DECRAMER, PhD, Toulouse University Hospital

Study record dates

Study Major Dates

• Study Start: June 1, 2013
• Primary Completion (Actual): May 25, 2016
• Study Completion (Actual): May 25, 2016

Study Registration Dates

• First Submitted: June 6, 2014
• First Submitted That Met QC Criteria: June 12, 2014
• First Posted (Estimate): June 16, 2014

Study Record Updates

• Last Update Posted (Actual): September 23, 2020
• Last Update Submitted That Met QC Criteria: September 21, 2020
• Last Verified: September 1, 2020

More Information

Terms related to this study

• Keywords: diabetic nephropathy; Microalbuminuria; type I diabetes; early predictive urinary biomarkers
• Additional Relevant MeSH Terms: Glucose Metabolism Disorders; Metabolic Diseases; Immune System Diseases; Autoimmune Diseases; Urologic Diseases; Endocrine System Diseases; Diabetes Complications; Diabetes Mellitus; Kidney Diseases; Diabetes Mellitus, Type 1; Diabetic Nephropathies
• Other Study ID Numbers: 12 356 03; 2012-A01097-36 (Registry Identifier: ID-RCB)

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No