Protein Ingestion Before Training Low

Nutritional Strategies for Optimizing Adaptations and Recovery Following Training 'Low'.

A randomized interventions study using a crossover design. Ten well trained triathletes or bikeriders will be included for to undergo two trail separated by about 3 weeks. The night before the trail each subject will performe a high intense interval trainings session on an ergometer bike. during the night an infusion of aminoacid tracers will be initiated. The subject is woken at 6.30 a.m. Blood samples and a musclebiopsy will be collected prior to the morning bike ride at 8.00a.m. Immediately before the morning bike ride is started the subject will ingest a drink of 0,5g protein or a non-caloric placebo (random order). During the bike ride and the following 4,5 hours blood will be drawn with regular intervals. at time point 0, 60 and 180 min after the training session biopsies are collected.

Study Overview

• Status: Completed
• Conditions: Protein Metabolism
• Intervention / Treatment: Other: Protein ingestion; Other: Non-caloric placebo ingestion

• Study Type: Interventional
• Enrollment (Actual): 9
• Phase: Not Applicable

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 50 years (ADULT)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• Healthy well-trained triathletes or cyclists
• VO2max > 55 ml O2/kg/min
• Training and has been training more than 7 times a week the last 6 months.

Exclusion Criteria:

• VO2max < 55 ml O2/kg/min
• Has chronic disease
• has incjected with tracer on a previous occasion.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: HEALTH_SERVICES_RESEARCH
• Allocation: RANDOMIZED
• Interventional Model: CROSSOVER
• Masking: SINGLE

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
EXPERIMENTAL: Protein ingestion; Subjects ingested protein drinks	Other: Protein ingestion; A protein drink was ingested prior to the 90min bike ride in one of the completed trails (order randomised).
PLACEBO_COMPARATOR: Placebo; Subjects ingested a non-caloric placebo drink	Other: Non-caloric placebo ingestion; A placebo drink was ingested prior to the 90min bike ride in one of the completed trails (order randomised).

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Muscle protein fractional synthetic rate (FSR)	FSR was measure over at time period of six hours

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Tracer kinetics dilution model	A-V tracer dilution model was applied across the forearm.	six hours
Muscle signalling	Muscle signalling will be determined using western blotting in each muscle biopsy	Determined in biopsies sampled before and immediatly after 90min exercise in addition to one and four hours after exercise.
Nitrogen excretion	nitrogen excretion is determined by Plasma and urinary carbide analysis as well as plasma NH4+.	Urine is collected during two periods. Collection period one: 12 hours prior to morning exercise. Collection period 2: During and four hours following exercise. Blood samples is drawn repeatedly from before to four hours after exercise.

Collaborators and Investigators

• Sponsor: University of Aarhus
• Collaborators: Innovation Fund Denmark; Arla Foods; Team Denmark

Publications and helpful links

General Publications

• Larsen MS, Holm L, Svart MV, Hjelholt AJ, Bengtsen MB, Dollerup OL, Dalgaard LB, Vendelbo MH, van Hall G, Moller N, Mikkelsen UR, Hansen M. Effects of protein intake prior to carbohydrate-restricted endurance exercise: a randomized crossover trial. J Int Soc Sports Nutr. 2020 Jan 28;17(1):7. doi: 10.1186/s12970-020-0338-z.

Study record dates

Study Major Dates

• Study Start (ACTUAL): April 30, 2016
• Primary Completion (ACTUAL): January 10, 2017
• Study Completion (ACTUAL): January 10, 2017

Study Registration Dates

• First Submitted: May 8, 2017
• First Submitted That Met QC Criteria: May 8, 2017
• First Posted (ACTUAL): May 10, 2017

Study Record Updates

• Last Update Posted (ACTUAL): May 10, 2017
• Last Update Submitted That Met QC Criteria: May 8, 2017
• Last Verified: May 1, 2017

More Information

Terms related to this study

• Keywords: Endurance exercise; Dietary protein
• Other Study ID Numbers: 50968

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No