- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT03855345
Antimicrobial Photodynamic Therapy Mediated by PapaMBlue® on Chronic Periodontal Disease
Antimicrobial Photodynamic Therapy Mediated by PapaMBlue® on Chronic Periodontal Disease - Randomized Blind Controlled Trial
Study Overview
Status
Conditions
Intervention / Treatment
Study Type
Enrollment (Anticipated)
Phase
- Phase 2
Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Genders Eligible for Study
Description
Inclusion Criteria:
- The patient must have chronic periodontitis (AAP, 2001);
- At least 10 present teeth, with at least 3 sites with clinical depth of probing greater than 4 mm;
- The patient must be in periodontal treatment at the Dental Clinic of Universidade Nove de Julho, institution where this research will be carried out;
Exclusion Criteria:
- Smokers or former smokers who discontinued the habit less than 12 months prior to selection;
- Uncontrolled diabetes;
- Anemia;
- Cancer;
- Pregnant women;
- Use of antibiotics in the last 6 months;
- Use of anti-inflammatory in the last 3 months;
- Coagulation disorder (use of anti-coagulant, presence of liver diseases, thrombocytopenia and immunosuppression);
- Patients under orthodontic treatment;
- Patients who maintain a biofilm index greater than 25%.
Study Plan
How is the study designed?
Design Details
- Primary Purpose: Treatment
- Allocation: Randomized
- Interventional Model: Parallel Assignment
- Masking: Single
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
Experimental: Control group
In this group, the participants will be submitted to the conventional treatment.
The treatment will consist of oral hygiene orientation, with instructions of brushing technique and recommendation of the daily use of dental floss.
All patients will receive a demonstration of oral hygiene techniques.
The calculus deposits on the teeth will be removed with ultrasound equipment and curettes for root scaling and straightening.
After the use of ultrasound and curettes, bicarbonate jet will be used to remove dental biofilm.
Treatment will be performed in 2 to 4 sessions under local anesthesia (typically 2% mepivacaine with 1: 100,000 noradrenaline).
Gracey periodontal curettes (numbers 3/4, 7/8, 11/12 and 13/14) and Mc Call curettes for removal of the dental calculus will be used.
Other biofilm-retaining factors, such as carious lesions, condemned teeth, and ill-adapted restorations, will be removed during these periodontal treatment sessions.
|
The treatment will consist of oral hygiene orientation, with instructions of brushing technique and recommendation of the daily use of dental floss.
All patients will receive a demonstration of oral hygiene techniques.
The calculus deposits on the teeth will be removed with ultrasound equipment and curettes for root scaling and straightening.
After the use of ultrasound and curettes, bicarbonate jet will be used to remove dental biofilm.
Treatment will be performed in 2 to 4 sessions under local anesthesia (typically 2% mepivacaine with 1: 100,000 noradrenaline).
Gracey periodontal curettes (numbers 3/4, 7/8, 11/12 and 13/14) and Mc Call curettes for removal of the dental calculus will be used.
Other biofilm-retaining factors, such as carious lesions, condemned teeth, and ill-adapted restorations, will be removed during these periodontal treatment sessions.
|
Experimental: aPDT group
In this group, besides the conventional treatments, patients will also receive aPDT.
aPDT will be performed at the end of each periodontal treatment session, at sites with bags greater than or equal to 4 mm.
The photosensitizer PapaMBlue® (F & A Ltda, São Paulo, Brazil) will be deposited in the pouches with a syringe, with the application in coronal direction, and a time of 1 min of pre-irradiation will be adopted so that the substance can stain bacterial biofilm (according to the manufacturer's information).
Next, the diode laser emitting wavelength of ʎ=660 nm, with power of P=100 mW, will be applied.
The laser will be applied to the mucosa, over the oral epithelium with an optical fiber.
Irradiation will be performed until the entire periodontal pocket is illuminated for 2 min at each point.
Each irradiation point will be approximately 0.4cm2, which will result in energy density of 30 J/cm2 in 2 min irradiation.
The irradiation will have a constant power density of 250 mW/cm2.
|
The treatment will consist of oral hygiene orientation, with instructions of brushing technique and recommendation of the daily use of dental floss.
All patients will receive a demonstration of oral hygiene techniques.
The calculus deposits on the teeth will be removed with ultrasound equipment and curettes for root scaling and straightening.
After the use of ultrasound and curettes, bicarbonate jet will be used to remove dental biofilm.
Treatment will be performed in 2 to 4 sessions under local anesthesia (typically 2% mepivacaine with 1: 100,000 noradrenaline).
Gracey periodontal curettes (numbers 3/4, 7/8, 11/12 and 13/14) and Mc Call curettes for removal of the dental calculus will be used.
Other biofilm-retaining factors, such as carious lesions, condemned teeth, and ill-adapted restorations, will be removed during these periodontal treatment sessions.
aPDT will be performed at the end of each periodontal treatment session, at sites with bags greater than or equal to 4 mm.
The photosensitizer PapaMBlue® (F & A Ltda, São Paulo, Brazil) will be deposited in the pouches with a syringe, with the application in coronal direction, and a time of 1 min of pre-irradiation will be adopted so that the substance can stain bacterial biofilm (according to the manufacturer's information).
Next, the diode laser emitting wavelength of ʎ=660 nm, with power of P=100 mW, will be applied.
The laser will be applied to the mucosa, over the oral epithelium with an optical fiber.
Irradiation will be performed until the entire periodontal pocket is illuminated for 2 min at each point.
Each irradiation point will be approximately 0.4cm2, which will result in energy density of 30 J/cm2 in 2 min irradiation.
The irradiation will have a constant power density of 250 mW/cm2.
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Change in probing depth
Time Frame: Baseline, 30, 60 and 90 days after treatment.
|
For the evaluation of probing depth, a single trained, calibrated examiner will examine 6 sites of each tooth with a 15mm North Carolina millimeter probe (Hu-Friedy, Chicago, IL, USA) for the measurement of probing depth in milimeters.
The evaluation will be performed at the beginning of treatment, 30, 60 and 90 days after the tretaments.
|
Baseline, 30, 60 and 90 days after treatment.
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Change in probing bleeding
Time Frame: Baseline, 30, 60 and 90 days after treatment.
|
For the evaluation of bleeding, a single trained, calibrated examiner will examine 6 sites of each tooth using a 15mm North Carolina millimeter probe (Hu-Friedy, Chicago, IL, USA) to check whether there is bleeding during the probing.
The evaluation will be performed at the beginning of treatment, 30, 60 and 90 days after the treatments.
|
Baseline, 30, 60 and 90 days after treatment.
|
Change in loss of clinical insertion
Time Frame: Baseline, 30, 60 and 90 days after treatment.
|
A single trained, calibrated examiner will examine 6 sites in each tooth with a 15mm North Carolina millimeter probe (Hu-Friedy, Chicago, IL, USA) for the measurement of loss of clinical insertion in milimeters.
The evaluation will be performed at the beginning of treatment, 30, 60 and 90 days after the treatments.
|
Baseline, 30, 60 and 90 days after treatment.
|
Change in plaque index
Time Frame: Baseline, 30, 60 and 90 days after treatment.
|
For the evaluation of plaque index, a single trained, calibrated examiner will examine 6 sites of each tooth with a 15mm North Carolina millimeter probe (Hu-Friedy, Chicago, IL, USA) for the measurement of the amount of plaque in milimeters.
The evaluation will be performed at the beginning of treatment, 30, 60 and 90 days after treatment.
|
Baseline, 30, 60 and 90 days after treatment.
|
Microbiological examination (change in CFUs (Colony Forming Units))
Time Frame: Baseline, 30, 60 and 90 days after treatment.
|
The microbiological examination will be performed from subgingival biofilm samples collected from the periodontal pockets. Two collections will be performed at each experimental site before and immediately after treatments. For the collection of the subgingival biofilm, a relative isolation of the teeth with cotton rollers will be performed, the supra-gingival biofilm will be removed with sterile gauze, and the subgingival biofilm sample will be obtained by inserting a sterile absorbent paper tip (no. 30) into the inside of the pocket, being held in place for 30s. The tips will be removed and stored in properly identified sterile plastic microtubes, with each paper cone being stored in a different microtube. The samples will be used to determine the CFUs (Colony Forming Units). |
Baseline, 30, 60 and 90 days after treatment.
|
Collaborators and Investigators
Sponsor
Study record dates
Study Major Dates
Study Start (Anticipated)
Primary Completion (Anticipated)
Study Completion (Anticipated)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Actual)
Study Record Updates
Last Update Posted (Actual)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Additional Relevant MeSH Terms
Other Study ID Numbers
- GIU
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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