Extracorporeal Cytokine Removal in Patients With Septic Shock in an Adult Intensive Care Unit

Goals Primary: compare changes in norepinephrine requirements before and after hemoperfusion (HP) treatment.

Secondary: demonstrate the decrease in levels of IL-6 , assess the total and individual change of SEQUENTIAL ORGAN FAILURE ASSESSMENT (SOFA), establish the resolution of shock , clearance of lactate , and mortality at discharge from INTENSIVE CARE UNIT (ICU), at 30 and 60 days.

Study Overview

• Status: Unknown
• Conditions: Septic Shock

Detailed Description

The study will take place at UTI Dr. José María Cullen Hospital in the city of Santa Fe. An observational study will be carried out in adult patients with septic shock (according to the definition of Sepsis-3), presenting hypotension, requiring vasopressors to maintain a mean arterial pressure (MAP) of 65 mmHg, blood lactate> 2 mmol / L, after adequate resuscitation with fluids and in need of extracorporeal membrane oxygenation (Extracorporeal Membrane Oxygenation, ECMO), Renal Replacement Therapy (TRR) ( which may be continuous (Continuous Renal Replacement Therapy (CRRT)) or Extended Slow Daily Dialysis (SLEDD) or both, added to the hemoperfusion (HP) treatment.

The study was designed by the authors, without receiving any input from companies.

Patients will be treated according to The Surviving Sepsis Campaign 2016 recommendations: identification and control of the infectious focus (surgery). It will begin with antibiotics as soon as possible, resuscitation with crystalloids at 30 mL / kg in case of hypotension and continue according to assessment of dynamic and static parameters (EV 1000, Swan Ganz, central venous pressure and echocardiography, used at the discretion of attending physician). Noradrenaline will be administered to maintain a MAP ≧ 65 mmHg. Dobutamine will be indicated if the minute volume <3.0 L / min / m2. According to our practice, adrenaline will be added if the dose of norepinephrine exceeds 2 μg / kg / min. In addition, 100 mg of hydrocortisone will be administered every 8 hours.

In case of requiring TRR for AKI, continuous vein-venous hemodiafiltration will be started using Prismaflex or Amplya equipment with a dose of 35 mL / kg / h, at a 1: 1 ratio, the replacement solution / dialysate solution, with AN ST 1.5 filter, HF 1.4 or HFT 1.4 or 1.7 or 2.2 with citrate as an anticoagulation method. In the case of ECMO, anticoagulation will be performed with heparin, according to the standardized practice of the service.

Treatment with HA 330 cartridges: Hemoperfusion treatment will be initiated as soon as possible, no later than 12 hours after the diagnosis of septic shock. An 8-hour treatment will be indicated for 2 (two) consecutive days. Prior to using the cartridge, 12,500 units of sodium heparin will be placed inside through the hole located in the upper region. During the next 30 minutes, it will be necessary to rotate it so that heparin is distributed throughout its interior. After 30 minutes, a physiological solution should be washed (a total of 2000 mL, with an infusion pump or by gravity pump) in order to remove the heparin, and in turn, avoid the formation of bubbles or air accumulation. After this step it is ready to be used, either for isolated HP therapies or in association with other therapies (SLEDD, ECMO, CRRT )

Data collection: For each patient who enters the protocol, a record will be completed with the data from the sampling, date and time of extraction, with the results of IL-6 and PCT and with the clinical data. The latter will be:

• age
• sex
• preexisting diseases
• infectious focus
• community or acquired sepsis
• positive crops
• SOFA score (daily for 7 (seven) days)
• APACHE II (upon admission and before starting HP treatment)
• IL-6 (as admission criteria and before and after each treatment)
• procalcitonin (PCT) on admission and on days 5-7
• lactate (before and after treatment with HP and daily for 7 (seven) days)
• additional laboratory: blood count, hepatogram, urea, creatinine, coagulogram, norepinephrine / adrenaline / IDI dose
• antibiotic dosing (according to the established sampling scheme)
• administered volume of fluids (daily for 7 (seven) days
• requirement of TRR and ECMO.

Informed consent will be obtained for each patient who enters the protocol for participation Samples will be taken at predetermined times for 48-72 hours following the established procedure.

• Study Type: Observational
• Enrollment (Anticipated): 20

Contacts and Locations

Study Locations

• Argentina
  • Santa Fe, Argentina, 3000
    • Recruiting
    • Hospital Dr Jose Maria Cullen
    • Contact: RAFAEL AVILA, MD; Phone Number: +5493425012511; Email: rafadata1@hotmail.com
    • Contact: MARIA FILIPPI, MD; Phone Number: +5493425290054; Email: utifilipi@yahoo.com.ar

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 99 years (ADULT, OLDER_ADULT)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Non-Probability Sample

Study Population

An observational study will be carried out in adult patients with septic shock (according to the definition of Sepsis-3), presenting hypotension, requiring vasopressors to maintain a mean arterial pressure (MAP) of 65 mmHg, blood lactate> 2 mmol / L, after adequate fluid resuscitation and in need of extracorporeal membrane oxygenation (Extracorporeal Membrane Oxygenation, ECMO), Renal Replacement Therapy (TRR) (which may be continuous (Continuous Renal Replacement Therapy (CRRT)) or Extended Slow Daily Dialysis ( Slow Extended Dialy Dialysis (SLEDD)) or both, added to the hemoperfusion (HP) treatment.

Description

Inclusion Criteria:

1.> 18 years 2. Diagnosis of septic shock (according to sepsis-3 definition) 3. Dose of norepinephrine ≧ 0.4 μg / kg / min 4. Plasma levels of IL-6> 900 pg / mL 5. Procalcitonin (PCT) ≧ 2 ng / mL 6. Need for renal replacement therapies or ECMO

-

Exclusion Criteria:

1. Neoplastic disease with or without treatment
2. Pregnant or lactating women (human chorionic gonadotrophin (hCG) test will be performed on potential patients before recruiting for treatment) -

Study Plan

How is the study designed?

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
changes in norepinephrine requirements meassured in micrograms/kg/ min ( gamma) before and after hemoperfusion (HP) treatment.	we will meassure norepinephrine requirements by placing arterial monitoring during hemoperfusion procedure	8 hours

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
levels of IL-6	measured by electrochemiluminescence ( picograms/ mililiter)	8 hours
changes of Sequential Organ Failure Assessment ( SOFA)	meassured by scale from 0 to 24 where the highest value is worst	8 hours
concentration of lactate clearence	(initial lactate - lactate at the end of treatment) / initial lactate x 100	8 hours
mortality at discharge from UTI		7 days; 30 days , 60 days

Collaborators and Investigators

• Sponsor: Hospital Jose Maria Cullen

Study record dates

Study Major Dates

• Study Start (ACTUAL): December 1, 2019
• Primary Completion (ANTICIPATED): December 1, 2020
• Study Completion (ANTICIPATED): December 1, 2021

Study Registration Dates

• First Submitted: December 28, 2019
• First Submitted That Met QC Criteria: January 14, 2020
• First Posted (ACTUAL): January 18, 2020

Study Record Updates

• Last Update Posted (ACTUAL): January 18, 2020
• Last Update Submitted That Met QC Criteria: January 14, 2020
• Last Verified: January 1, 2020

More Information

Terms related to this study

• Keywords: septic shock; ECMO; CRRT; hemoperfusion; MODS
• Additional Relevant MeSH Terms: Pathologic Processes; Infections; Systemic Inflammatory Response Syndrome; Inflammation; Sepsis; Shock, Septic; Shock
• Other Study ID Numbers: cytokine

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No