- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT04245332
Effects of Fish Oil and Resistance Training on Muscle Mass and Function
Effects of Fish Oil Supplementation in Combination With Resistance Exercise Training on Body Composition, Strength and Neuromuscular Function in Young, Resistance-Trained Individuals: A Randomized, Open-Label, Placebo-Controlled Trial
Study Overview
Status
Conditions
Intervention / Treatment
Detailed Description
Potential participants will report to the Human Performance Lab (HPL) in James Hall to complete the screening process. They will complete the informed consent form process with a member of the research team before providing any protected health information (PHI). The screening process has two components: questionnaires and physical measures. Potential participants will complete the following questionnaires: Stanford 7-Day Physical Activity Questionnaire and a Medical History Questionnaire. Participants will complete the following physical measures: height, weight, waist and hip circumference, and blood pressure will be assessed and followed by calculations of body mass index (BMI) and waist to hip ratio (WHR). The final measure will be a determination of fasting total cholesterol and blood glucose measured via capillary blood from a finger prick using an automated analyzer. The purpose of the screening process is to determine if a participant is healthy and can safely participate in exercise testing, and to determine their eligibility to participate in the study based on the criteria listed elsewhere.
Participants who meet the eligibility criteria and are willing to participate will be enrolled in the study and randomly assigned to FO or PLA via block randomization methods. Enrolled participants will return to the HPL in the morning to complete a battery of biometric tests (described below) and will be given two logs to complete to collect baseline data: the Stanford 7-Day Physical Activity Questionnaire and a 3-day diet log. The purpose of the physical activity log is to determine their average level of physical activity and training program. The purpose of the 3-day diet log is to record their normal dietary intake and to provide practice for completing these logs as they will be completed on a weekly basis during the supplement intervention period.
Participants will have the following physical measures recorded: height, weight, waist and hip circumference, body composition, and blood pressure. Participants will then have their resting metabolic rate (RMR) measured by indirect calorimetry. In preparation for this test participants will be asked to complete an overnight fast of at least 9 hours, abstain from caffeine for 12 hours and abstain from exercise and alcohol for 24 hours. Participants will be brought to a quiet room and asked to lie in a supine position for 30 minutes of rest. They will then be fitted with a breathing mask connected to the metabolic cart and their breath will be analyzed for 15 minutes while they continue to lie in a supine position. Their total energy expenditure will be calculated using their RMR and a physical activity factor based on their level of physical activity obtained from the Stanford 7-Day Physical Activity Questionnaire. Subject's total daily calorie requirements will then be verified using their 3-day diet record and the Mifflin-St. Jeor prediction equation. In order to ensure that participants are consuming enough calories in order to maintain their body weight during the 6-week supplementation period, the research team will use the average of participant's total calorimetric data (based on RMR), 3-day diet log, and the Mifflin-St. Jeor estimate.
Participants will be sent pre-participation instructions before testing to ensure they arrive at the HPL with proper exercise attire and having abstained from caffeine for 12 hours and exercise and alcohol for 24 hours. They will report to the HPL to complete a 1RM test, a type of muscular strength assessment. This test will be performed for the back squat in a Smith machine with hand release breaks. Water will be provided and consumed ad libitum. Bench press and Squat will be completed with proper lifting technique as described by the National Strength and Conditioning Association (NSCA) guidelines.
Participants' lower body neuromuscular function, assessed via maximal voluntary contraction (MVC) and rate of force development (RFD), will be completed through the use of force transducers applied to the legs. Participants will be seated in a custom-made wooden box with a single axis transducer attached to one of its surfaces (SM-500, Interface Inc., Scottsdale, Arizona, USA). When subjects sit on the box, their knee and hip joints will be at approximately 70° and 90° of flexion from full extension, respectively. Subjects will be asked to cross their arms across their chest during the testing.
Dietary compliance will be monitored weekly during the free-living supplementation period using 3-day diet records and returned supplement pill bottles. 3-day diet records will be recorded by the participants and returned to the research team for analysis using professional nutrition analysis software (Food Processor, Oregon). Dietary analysis results will be discussed with the participant in order to help them adhere to dietary recommendations. Body weight measures will be recorded once per week to ensure a stable weight during the dietary intervention period. The participants will return supplement bottles weekly, and the research team will count and record any remaining capsules. Finally, physical activity logs will be collected every week to ensure diet and calorie recommendations are meeting the demands of RET.
Study Type
Enrollment (Anticipated)
Phase
- Not Applicable
Contacts and Locations
Study Locations
-
-
New Jersey
-
Glassboro, New Jersey, United States, 08028
- Rowan University
-
-
Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Genders Eligible for Study
Description
Inclusion Criteria:
- Young, healthy, resistance-trained men and women of all nationalities between the ages of 18-35
Exclusion Criteria:
- metabolic disease (diabetes or metabolic syndrome)
- cardiovascular disease
- pulmonary disease
- chronic inflammatory conditions (e.g. renal/kidney, liver, asthma, arthritis, autoimmune diseases, inflammatory bowel disease)
- neurological conditions (e.g. Parkinson's)
- sexually transmitted diseases
- cancer diagnosis within the previous 5 years
- currently smoking cigarettes
- hypertension
- hypercholesterolemia and/or dyslipidemia
- using medications to manage cholesterol, blood lipids, blood pressure, or diabetes
- taking performance-enhancing drugs and/or anabolic agents (e.g. testosterone, growth hormone, or beta-2 agonists)
- oral contraceptive use, are pregnant, or intend to become pregnant while enrolled in the study
- fish oil supplement use within the past 6 months
- fasting blood total cholesterol concentration >200mg/dl
- fasting blood glucose concentration >100mg/dl
Study Plan
How is the study designed?
Design Details
- Primary Purpose: Basic Science
- Allocation: Randomized
- Interventional Model: Parallel Assignment
- Masking: Single
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
Experimental: Fish Oil
This group will receive fish oil (4g/d).
|
Fish oil-derived omega-3 polyunsaturated fatty acids (4g fish oil concentrate: 2g EPA+ 1g DHA)
Other Names:
|
Placebo Comparator: Placebo
This group will receive coconut oil (4g/d) as an iso-energetic, iso-lipidic placebo comparator to the fish oil arm.
|
Coconut oil (4g/d)
Other Names:
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Total body fat-free mass (FFM)
Time Frame: 6 weeks
|
Measured by bioelectrical impedance analysis (BIA)
|
6 weeks
|
Muscle strength
Time Frame: 6 weeks
|
Measured by one-repetition maximal (1RM) strength in back squat, bench press, and knee extensor
|
6 weeks
|
Rate of force development (RFD)
Time Frame: 6 weeks
|
Assessed by force transducers on legs and in hands
|
6 weeks
|
Maximal voluntary contraction (MVC)
Time Frame: 6 weeks
|
Assessed by force transducers on legs and in hands
|
6 weeks
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Red blood cell omega-3 fatty acid concentrations (Eicosapentaenoic acid [EPA] and docosahexaenoic acid [DHA])
Time Frame: 6 weeks
|
Assessed by gas chromatography-mass spectrometry (GS-MS)
|
6 weeks
|
Collaborators and Investigators
Sponsor
Investigators
- Principal Investigator: Dylan Klein, Ph.D., Rowan University
Study record dates
Study Major Dates
Study Start (Actual)
Primary Completion (Anticipated)
Study Completion (Anticipated)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Actual)
Study Record Updates
Last Update Posted (Actual)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Other Study ID Numbers
- Pro2019000649
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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