Study of Anti-CD33/CLL1 CAR-NK in Acute Myeloid Leukemia

Clinical Study of Conjugated Antibody Redirecting Natural Killer (CAR-NK) Cells Targeting CD33 and CLL1 in Patients With Acute Myeloid Leukemia

This is an open-label, nonrandomized, investigator-initiated clinical trial to evaluate the safety, tolerability, pharmacokinetics, and efficacy of anti-CD33/CLL1 CAR-NK cell injection in patients with acute myeloid leukemia (AML), and to determine PK parameters, maximum tolerated dose (MTD), and phase II recommended dose (RP2D) for subjects receiving CAR-NK cell injection.

Study Overview

• Status: Recruiting
• Conditions: Acute Myeloid Leukemia
• Intervention / Treatment: Biological: Anti-CD33/CLL1 CAR-NK Cells

Detailed Description

The treatment cycle in this study is 28 days. The administration of CAR-NK cells will be performed on day 1 and day 3 of each cycle. Subjects will be treated continuously until the criteria for termination of treatment are met. In this study, the dose escalation design is adopted. The first administration dose in the first cycle is 2.0×10^9 cells. If no adverse events were observed, the second administration dose in the first cycle would be 3.0×10^9 cells, and each administration dose in the second cycle and thereafter would be 3.0×10^9 cells.

• Study Type: Interventional
• Enrollment (Anticipated): 18
• Phase: Early Phase 1

Contacts and Locations

• Study Contact: Name: Peihua Lu, MD; Phone Number: +8613583991399; Email: lyzlyylxm@163.com
• Study Contact Backup: Name: Xin Zhou, MD; Phone Number: +8613358111962; Email: 13625653@qq.com

Study Locations

• China
  • Jiangsu
    • Wuxi, Jiangsu, China
      • Recruiting
      • Wuxi People's Hospital
      • Contact: Peihua Lu, MD; Phone Number: +8613583991399; Email: lyzlyylxm@163.com

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 16 years to 73 years (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria:

1. Subjects should voluntarily participate in this clinical study, are fully aware of the study, have signed the Informed Consent Forms, and are willing to follow and able to complete all trial procedures.
2. Subjects who are more than 18 years old (including 18 years old), and less than 75 years old (including 75 years old);

3. Subjects who are diagnosed as AML according to the World Health Organization (WHO) 2016 diagnosis criteria, and meet any of the following:

   • Relapsed acute myeloid leukemia: after complete response (CR), there are leukemia cells in peripheral blood or blast cells in bone marrow >5% (except for other reasons such as bone marrow regeneration after consolidation chemotherapy) or extramedullary leukemia cell infiltration.
   • Refractory acute myeloid leukemia: treatment-naive subjects who have no response to the standard of care for 2 courses of treatment; subjects who are relapsed within 12 months with consolidated intensive treatment after CR; subjects who are relapsed after 12 months but have no response to the conventional chemotherapy; subjects who are relapsed twice or more; subjects who have persistent extramedullary leukemia.
4. Subjects who are positive in CD33 or CLL1 test (according to the results of the central laboratory);
5. Subjects are allowed to have received ≤1 autologous HSCT;
6. Subjects whose performance status scores of the Eastern Cooperative Oncology Organization (ECOG) are≤2;
7. Subjects with the expected survival phase>3 months;

8. Organ function should meet the following criteria:

   • Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) ≤ 3 times the upper limit of normal (ULN), unless these are caused by leukemia cell infiltration as determined by the investigator;
   • Total serum bilirubin ≤ 1.5 times the upper limit of normal (ULN);
   • Glomerular filtration rate (GFR)>50 mL/min;
   • Left ventricular ejection fraction (LVEF)≥50%, no clinically significant pericardial effusion confirmed by echocardiography;
   • Blood oxygen saturation under indoor ventilation in the screening phase>92%;
9. Female subjects of childbearing age must be negative in the pregnancy test results in the screening phase;
10. Male and female subjects of childbearing age must agree to take effective birth controls after signing the informed consent forms, during the study, and within 6 months after the last administration.

Exclusion Criteria:

1. Subjects who are known to have acute promyelocytic leukemia;
2. Subjects who suffer from or are suspected of suffering from central nervous system leukemia, or central nervous system involvement;
3. Subjects who have received allogeneic HSCT;
4. Subjects who have prior II-IV (Glucksberg criteria) acute graft versus-host disease (GvHD) or extensive chronic GvHD;
5. Subjects who have received chimeric antigen receptor cell therapy or other cell therapy;
6. Subjects who have received anti-tumor therapy in the early stage but are not recovered in the toxicity (according to NCI-CTCAE 5.0, the toxicity has not recovered to ≤ Grade 1, except for fatigue, anorexia, and alopecia);
7. Subjects who have had other malignant tumors within 5 years before inclusion, except for any type of carcinoma in situ that has been cured in the past and cured skin basal cell carcinoma or skin squamous cell carcinoma;

8. Subjects whose cardiac function and disease meet one of the following conditions within 6 months before the first administration:

   • Any risk factors that increase QTcF (Fridericia formula) interval prolongation, such as uncorrectable hypokalemia, hereditary long QT syndrome, and use of drugs that prolong the QTcF interval;
   • New York Heart Association (NYHA) classification ≥Grade 3;
   • QTcF interval prolongation (male>450 milliseconds; female>470 milliseconds);
   • Unstable angina pectoris, and myocardial infarction;
9. Subjects who have a history of autoimmune diseases (such as Crohn's disease, rheumatoid arthritis, systemic lupus erythematosus, etc.) that require systemic immunosuppressive/systemic disease modulating drugs within 2 years before the first administration;
10. Subjects who have active systemic fungal, bacterial or viral infections that are uncontrolled or are required to be treated by intravenous administration;
11. Subjects with human immunodeficiency virus (HIV) antibody positive;
12. Subjects with HBsAg (-) and HBcAb (-). If subjects have HBsAg (+) and/or HBcAb (+), then subjects with HBV-DNA below the local lower limit of quantification can be included;
13. Subjects with hepatitis C virus antibody (-). If subjects have hepatitis C virus antibody (+), then subjects with HCV-RNA (-) can be included;
14. Female subjects who are breastfeeding and unwilling to stop breastfeeding;
15. Subjects who have received major surgery (for the definition of major surgery, refer to the Level 3 and 4 surgeries specified in the Administrative Measures for the Clinical Application of Medical Technology) within 28 days before the first administration.
16. Subjects who have received a solid organ transplant;
17. Subjects who have a history of alcohol, drug use or drug abuse;
18. Subjects who are known to be allergic to the study drug and/or main components and/or any excipients of the study drug;
19. Subjects who have other severe, acute, or chronic diseases or laboratory abnormalities that may increase the risk of participating in the study and receiving the study drug, or may interfere with the interpretation of study results.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: N/A
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

1

Arms and Interventions

Participant Group / Arm

Intervention / Treatment

Experimental: Anti-CD33/CLL1 CAR-NK Cells; The administration of CAR-NK cells will be performed on day 1 and day 3 of each cycle (28 days). The first administration dose in the first cycle is 2.0×10^9 cells. If no adverse events were observed, the second administration dose in the first cycle would be 3.0×10^9 cells, and each administration dose in the second cycle and thereafter would be 3.0×10^9 cells.

Biological: Anti-CD33/CLL1 CAR-NK Cells; The administration of CAR-NK cells will be performed on day 1 and day 3 of each cycle (28 days). The first administration dose in the first cycle is 2.0×10^9 cells. If no adverse events were observed, the second administration dose in the first cycle would be 3.0×10^9 cells, and each administration dose in the second cycle and thereafter would be 3.0×10^9 cells.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Incidence of dose limiting toxicity (DLT)	To evaluate the safety and tolerability of Anti-CD33/CLL1 CAR-NK cells	28 days after initial infusion

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Overall survival (OS)	To evaluate the effectivity of anti-CD33/CLL1 CAR-NK cells	Up to 1 year after infusion
Minimal residual disease (MRD)	To evaluate the effectivity of anti-CD33/CLL1 CAR-NK cells	Up to 1 year after infusion
Objective response rate (ORR)	To evaluate the effectivity of anti-CD33/CLL1 CAR-NK cells	Up to 1 year after infusion
Duration of overall response (DOR)	To evaluate the effectivity of anti-CD33/CLL1 CAR-NK cells	Up to 1 year after infusion
Plasma concentration of CAR-NK cells	Blood samples will be collected at specified time points to detect the number of CAR-NK cells in peripheral blood and to evaluate the PK parameters	One month after the last infusion
Cytokine release	Blood samples will be collected at specified time points to detect the cytokine (IL-6, TNF-α, IL-1ra, IL-10, IL-2, etc.) concentration (pg/mL)	One month after the last infusion
Adverse Events (AEs)	The incidence and severity of adverse events assessed according to Common Terminology Criteria for Adverse Events (NCI-CTCAE) v5.0, and the correlation between adverse events and CAR-NK cells	From day 1 to day 90 after the last infusion
Percentage of subjects receiving hematopoietic stem cell transplantation (HSCT)	To explore HSCT in subjects receiving CAR-NK cell therapy.	Up to 1 year after infusion

Collaborators and Investigators

• Sponsor: Wuxi People's Hospital
• Collaborators: Imbioray (Hangzhou) Biomedicine Co., Ltd.

Study record dates

Study Major Dates

• Study Start (Actual): November 30, 2020
• Primary Completion (Anticipated): November 30, 2022
• Study Completion (Anticipated): November 30, 2022

Study Registration Dates

• First Submitted: January 18, 2022
• First Submitted That Met QC Criteria: January 18, 2022
• First Posted (Actual): January 31, 2022

Study Record Updates

• Last Update Posted (Actual): January 31, 2022
• Last Update Submitted That Met QC Criteria: January 18, 2022
• Last Verified: January 1, 2022

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Neoplasms by Histologic Type; Neoplasms; Leukemia; Leukemia, Myeloid; Leukemia, Myeloid, Acute
• Other Study ID Numbers: IBR733-T01; WX-IBR-7 (Other Identifier: Wuxi People's Hospital)

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: No

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No