- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT05934188
Exploring the Gut-Brain Axis in Ageing and Neurodegeneration (GutBrain)
Role of the Gut-microbiota on Ageing and Neurodegeneration: a Clinical and Brain Imaging Study
Neurodegenerative diseases are a major health concern due to their growing societal implications and economic costs. The identification of early markers of pathogenic mechanisms is one of the current main challenges. The gut-brain axis has become a primary target because of its transversal role across the neurodegenerative spectrum and its effect on cognition. However, despite recent progress, how changes in the gut-microbiota composition can affect the human brain is still unclear.
The goal of this observational study is to characterise the gut-microbiota composition associated with alterations in brain structure and function during the ageing process and across neurodegenerative disorders. This is based on recent studies showing that changes in the human brain and in the microbiota composition, can indicate very sensitively and in a predictive way pathological development and, consequently, be used as markers of neurodegenerative diseases.
The main questions it aims to answer are:
- How variation in the gut-microbiota composition correlates with the normal brain ageing trajectory?
- How dysregulation in the gut-microbiota correlates with pathological changes in brain regions in specific neurodegenerative disorders?
- Can the impact of the gut-microbiota on the brain be modulated by blood biomarkers?
The investigators will recruit 40 young healthy participants, 40 old healthy participants, 40 participants with prodromal Alzheimer's Disease, 40 participants with Parkinson's Disease and 40 participants with Multiple Sclerosis.
Participants will undergo the following examinations:
- Magnetic Resonance Imaging
- Analysis of a stool sample
- Analysis of a blood sample
- Neuropsychological assessment
- Questionnaires on eating habits
Study Overview
Status
Detailed Description
Recent studies show that alterations in the microbiota profile has been observed in the ageing process and across neurodegenerative disorders and it has been associated with cognitive decline and disease-specific clinical symptoms.
The objective of this multicenter observational cross-sectional cohort study is to characterise how changes in the gut-microbiota profile may affect brain changes during the physiological ageing processes and across neurodegenerative disorders with different etiopathogenesis.
The investigators will combine novel magnetic resonance imaging and biological techniques to test these hypotheses:
- Specific functional and structural changes, which reflect unsuccessful compensatory mechanisms to counteract ageing, are associated with changes in the gut-microbiota composition.
- Neurodegenerative disorders (prodromal Alzheimer's Disease, Parkinson's Disease, Multiple Sclerosis) show unique changes in the gut-microbiome profile, associated with specific structural and functional brain changes.
- The microbiota profile characterizing the unsuccessful ageing and different neurodegenerative diseases is associated with alterations in blood biomarkers.
For this study the investigators plan to recruit 80 healthy subjects divided into two groups (40 subjects aged 20-50 years and 40 subjects aged 60-90 years) and 120 patients divided into three groups (40 patients with prodromal Alzheimer's Disease, 40 patients with Parkinson's Disease and 40 patients with Multiple Sclerosis).
All participants will undergo a multimodal Magnetic Resonance Imaging protocol to study the brain structure and function and a detailed neuropsychological protocol to assess cognitive functioning. In addition, stool and blood samples will be collected to investigate the gut-microbiota composition and the presence of inflammatory markers, respectively. Participants will also be asked to fill out questionnaires on eating habits.
There are no known risks or long-term side effects related to Magnetic Resonance Imaging. The performance of the examination does not involve physical or mental impairment.
The study does not directly benefit the participant. However, participation in the study will increase knowledge in the area of the relationship between the gut microbiota and the brain, providing potential new knowledge useful for preventing the risk of developing neurodegenerative processes.
The study takes place at San Camillo IRCCS S.r.l. (70 Alberoni street, Lido VE, 30126, Italy IT).
The study started on 01/05/2023 and the end is planned for 30/04/2026. The submitted study is funded by the Ministry of Health through a finalized research call won by Principal Investigator, Dr. Nicola Filippini, and approved by Ethics Committee for Clinical Trials of the Province of Venice and IRCCS San Camillo, Azienda ULSS 3 Serenissima.
Study Type
Enrollment (Estimated)
Contacts and Locations
Study Contact
- Name: Nicola Filippini
- Phone Number: +39 041 2207304
- Email: nicola.filippini@hsancamillo.it
Study Locations
-
-
-
Brescia, Italy, 25125
- Recruiting
- IRCCS Istituto Centro San Giovanni di Dio Fatebenefratelli
-
Contact:
- Moira Marizzoni
- Email: mmarizzoni@fatebenefratelli.eu
-
Sub-Investigator:
- Moira Marizzoni
-
Sub-Investigator:
- Annamaria Cattaneo
-
Venice, Italy, 30123
- Recruiting
- Università Ca' Foscari Venezia
-
Contact:
- Guido Caldarelli
- Email: guido.caldarelli@unive.it
-
Sub-Investigator:
- Guido Caldarelli
-
-
Venice
-
Venice-Lido, Venice, Italy, 30126
- Recruiting
- IRCCS San Camillo
-
Contact:
- Nicola Filippini
- Phone Number: +39 041 2207304
- Email: nicola.filippini@hsancamillo.it
-
Principal Investigator:
- Nicola Filippini
-
Sub-Investigator:
- Rita Barresi
-
Sub-Investigator:
- Lisa Longo
-
Sub-Investigator:
- Mattia Spagna
-
Sub-Investigator:
- Giulia Serafica
-
-
Participation Criteria
Eligibility Criteria
Ages Eligible for Study
- Adult
- Older Adult
Accepts Healthy Volunteers
Sampling Method
Study Population
Description
INCLUSION CRITERIA:
Healthy Young and Old Subjects:
- 20-50 or 60-90 years old
- Cognitively healthy (Mini-Mental State examination ≥ 26)
- Absence of significant neurological disorders
Patients with prodromal Alzheimer's Disease:
- Subjective cognitive complaint (corroborated by the informant)
- Episodic memory deficit on neuropsychological testing
- Clinical Dementia Rating = 0.5
- Mini-Mental State Examination (MMSE) > 23
- Independently functioning in activities of daily living
Patients with Parkinson's Disease:
- Recent diagnosis of Parkinson's Disease
- Mild-moderate score at the Unified Parkinson's Disease Rating Scale (UPDRS)
- Cognitively healthy (Mini-Mental State examination ≥ 26)
- In case of taking medications for Parkinson's Disease: stable dosage for at least 6 months
Patients with Multiple Sclerosis:
- Recent diagnosis of relapsing-remitting Multiple Sclerosis
- Expanded Disability Status Scale score ≤ 4.0
- Cognitively healthy (Mini-Mental State examination ≥ 26)
- In case of taking medications for Multiple Sclerosis: stable dosage for at least 6 months.
EXCLUSION CRITERIA:
For both healthy participants and patients:
- Contraindications to magnetic resonance imaging (metal implant in body, known claustrophobia, pacemakers)
- Severe comorbidities
- Antibiotics treatments over the last 3 months
Study Plan
How is the study designed?
Design Details
Cohorts and Interventions
Group / Cohort |
Intervention / Treatment |
---|---|
Young Healthy Subjects (N = 40)
|
The Magnetic Resonance Imaging protocol will comprise both structural and functional sequences.
Neuropsychological tests will be administered to participants to assess general cognitive state and a range of high-level cognitive functions (memory, executive, language).
In addition, disease-specific tests will be administered to patients to investigate disease staging and the level of disability and autonomy.
Information on eating habits will be derived from food questionnaires.
The Microbiome analyses will be derived from a stool sample (16S rRNA sequencing targeted metagenomic analyses).
Inflammatory markers will be evaluated in terms RNA expression level in plasma blood sample.
|
Old Healthy Subjects (N = 40)
|
The Magnetic Resonance Imaging protocol will comprise both structural and functional sequences.
Neuropsychological tests will be administered to participants to assess general cognitive state and a range of high-level cognitive functions (memory, executive, language).
In addition, disease-specific tests will be administered to patients to investigate disease staging and the level of disability and autonomy.
Information on eating habits will be derived from food questionnaires.
The Microbiome analyses will be derived from a stool sample (16S rRNA sequencing targeted metagenomic analyses).
Inflammatory markers will be evaluated in terms RNA expression level in plasma blood sample.
|
Patients with prodromal Alzheimer's Disease (N = 40)
|
The Magnetic Resonance Imaging protocol will comprise both structural and functional sequences.
Neuropsychological tests will be administered to participants to assess general cognitive state and a range of high-level cognitive functions (memory, executive, language).
In addition, disease-specific tests will be administered to patients to investigate disease staging and the level of disability and autonomy.
Information on eating habits will be derived from food questionnaires.
The Microbiome analyses will be derived from a stool sample (16S rRNA sequencing targeted metagenomic analyses).
Inflammatory markers will be evaluated in terms RNA expression level in plasma blood sample.
The Alzheimer's Disease biomarkers will be measured in the plasma of prodromal Alzheimer's Disease patients.
|
Patients with Parkinson's Disease (N = 40)
|
The Magnetic Resonance Imaging protocol will comprise both structural and functional sequences.
Neuropsychological tests will be administered to participants to assess general cognitive state and a range of high-level cognitive functions (memory, executive, language).
In addition, disease-specific tests will be administered to patients to investigate disease staging and the level of disability and autonomy.
Information on eating habits will be derived from food questionnaires.
The Microbiome analyses will be derived from a stool sample (16S rRNA sequencing targeted metagenomic analyses).
Inflammatory markers will be evaluated in terms RNA expression level in plasma blood sample.
|
Patients with Multiple Sclerosis (N = 40)
|
The Magnetic Resonance Imaging protocol will comprise both structural and functional sequences.
Neuropsychological tests will be administered to participants to assess general cognitive state and a range of high-level cognitive functions (memory, executive, language).
In addition, disease-specific tests will be administered to patients to investigate disease staging and the level of disability and autonomy.
Information on eating habits will be derived from food questionnaires.
The Microbiome analyses will be derived from a stool sample (16S rRNA sequencing targeted metagenomic analyses).
Inflammatory markers will be evaluated in terms RNA expression level in plasma blood sample.
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Brain structural and functional properties
Time Frame: Day 1
|
Brain structural and functional properties will be derived from a multi-modal Magnetic Resonance Imaging protocol.
|
Day 1
|
Microbiome profile
Time Frame: Day 1
|
Microbiome profile will be derived from a stool sample obtained from participants.
|
Day 1
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Cognitive functioning
Time Frame: Day 1
|
Cognitive functions will be measured using a neuropsychological protocol.
|
Day 1
|
Concentration of blood inflammatory markers
Time Frame: Day 1
|
A panel of key inflammatory mediators (for example, IFNgamma, IL-6, TNFalpha, IL1beta, IL10) will be evaluated in terms RNA expression level in plasma samples obtained from participants.
|
Day 1
|
Eating habits
Time Frame: Day 1
|
Information on eating habits will be derived from food questionnaires.
|
Day 1
|
Collaborators and Investigators
Investigators
- Principal Investigator: Nicola Filippini, IRCCS San Camillo, Venezia, Italy
Study record dates
Study Major Dates
Study Start (Actual)
Primary Completion (Estimated)
Study Completion (Estimated)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Actual)
Study Record Updates
Last Update Posted (Actual)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Keywords
Additional Relevant MeSH Terms
- Mental Disorders
- Pathologic Processes
- Brain Diseases
- Central Nervous System Diseases
- Nervous System Diseases
- Immune System Diseases
- Demyelinating Autoimmune Diseases, CNS
- Autoimmune Diseases of the Nervous System
- Demyelinating Diseases
- Autoimmune Diseases
- Neurocognitive Disorders
- Parkinsonian Disorders
- Basal Ganglia Diseases
- Movement Disorders
- Synucleinopathies
- Neurodegenerative Diseases
- Dementia
- Tauopathies
- Multiple Sclerosis
- Parkinson Disease
- Alzheimer Disease
- Nerve Degeneration
Other Study ID Numbers
- 2022.13
- RF-2021-12372224 (Other Grant/Funding Number: Ministry of Health)
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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