- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT05992727
Evaluation of Galectin-3 in Serum and Saliva of Pemphigus Patients
Evaluation of Galectin 3 Level in Serum and Saliva of Pemphigus Patients and Its Correlation With Disease Severity and Systemic Comorbidities
Study Overview
Status
Conditions
Intervention / Treatment
Detailed Description
Pemphigus is a potentially life- threatening immune mediated bullous disease that affects the skin and mucous membranes with a significant impact on quality of life (1). It is due to production of autoantibodies against desmogleins (adhesion molecules of the epidermis) leading to disruption of junctions between keratinocytes and subsequently acantholysis (2).
Pemphigus diseases are classified based on the clinical, histopathological features, as well as on the specific antigens against which the autoantibodies are produced. The main forms are pemphigus vulgaris (PV) and pemphigus folia¬ceus (PF), but other non-classical forms of pemphigus have also been described as paraneoplastic pemphigus, pemphigus herpetiformis, and IgA pemphigus (3). PV is the main clinical form of pemphigus, accounting for approximately 70% of cases; it is also considered the most severe form of the disease (4).
IgG is the main immunoglobulin seen along the dermal-epidermal junction in pemphigus patients, in addition most patients generate IgE autoantibody response. Soluble CD23 and galectin-3 are the two main elements of the IgE (5).
Galectin-3(Gal-3) is a beta galactoside-binding lectins that is essential in the cell-to-cell or matrix adhesion, as well as plays an important role in cell growth, differentiation, macrophage activation, antimicrobial activity, angiogenesis, and apoptosis. It broadly exists in the nucleus and cytoplasm of various cell types or may be found extracellularly on the cell surface (6) .Gal-3 is readily secreted to the cell surface and into biological fluids as serum, urine, tears and saliva from injured and inflammatory cells. So, it can be used as a sensitive diagnostic or prognostic biomarker for various pathological conditions (7).
Gal-3 has been used as a novel biomarker in the early detection of myocardial dysfunction and heart failure. Many cardiac biomarkers which reflect cardiac inflammation and fibrosis may also contribute to the progression of kidney disease (8, 9) A previous study reported a significant decrease in galectin-3 cytoplasmic and nuclear expression around blisters compared with adjacent unaffected skin in PV, which may play a role in extension of acantholysis (5). To the best of our knowledge, no previous studies assessed the role of Gal-3 in serum or saliva of pemphigus patients.
Study Type
Enrollment (Estimated)
Contacts and Locations
Study Contact
- Name: Eman Fathy, MD
- Phone Number: +201002258614
- Email: emanfathy639@yahoo.com
Study Contact Backup
- Name: Heba Hasan, MD
- Phone Number: +201002866919
- Email: hebahasan16888@yahoo.com
Participation Criteria
Eligibility Criteria
Ages Eligible for Study
- Child
- Adult
- Older Adult
Accepts Healthy Volunteers
Sampling Method
Study Population
Description
Inclusion Criteria:
Patients with active stage of pemphigus.
Exclusion Criteria:
- Patients who were on topical ttt, systemic corticosteroids or other immunosuppressive drugs in the last one month.
- Patients with disease attack duration more than 1 week.
Patients with any other concomitant dermatological or chronic systemic diseases.
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Study Plan
How is the study designed?
Design Details
Cohorts and Interventions
Group / Cohort |
Intervention / Treatment |
---|---|
Group 1( cases)
Galectin 3 will be measured in 30 Patients who are with active stage of pemphigus either newly diagnosed or in a relapse.
The diagnosis of each patient is based on clinical examination and histopathological examination.
|
Galectin 3 measurement in serum and saliva by ELISA in the two groups
Other Names:
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Group 2( controls)
Galectin 3 will be measured in30 age and sex matched healthy controls
|
Galectin 3 measurement in serum and saliva by ELISA in the two groups
Other Names:
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What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Assessment of Galectin 3
Time Frame: 1 year
|
Three millimeters of blood will be withdrawn aseptically from all patients and controls from the antecubital vein using disposable plastic syringe.
The sera will be separated and stored at -20 C. Saliva will be collected from patients and controls after asking them to refrain from eating and drinking (except for water) for 2 hours before collection.
Then, they will be asked to rinse their mouth with water, tilt their heads down, pool saliva in the mouth for 1 minute, drool into a sterile falcon tube.
Saliva will be separated and stored also at-20 C. Human Gal-3 ELISA kit will be used for quantitative measurement of serum and saliva Gal-3 levels.
The assay will be performed according to the manufacturer's instructions.
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1 year
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Develop a novel biomarker in serum and saliva of pemphigus patients and to detect its correlation with disease activity, as well as systemic comorbidities.
Time Frame: 1year
|
All patients will be subjected to complete history taking, meticulous general, dermatological examination and routine investigations.
Echocardiography, kidney function tests and urine analysis will be conducted for all the participants.
Disease severity will be assessed by ABSIS score with a maximum score of 206.
It uses the rule of 9s to assess the percentage of involvement of blisters and erosions on the skin combined with a weighting factor for the stage of the blistering and erosions.
Oral involvement is based on 2 scores comprising the extent (presence of lesions) and severity (discomfort during eating and drinking)
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1year
|
Collaborators and Investigators
Sponsor
Publications and helpful links
Study record dates
Study Major Dates
Study Start (Estimated)
Primary Completion (Estimated)
Study Completion (Estimated)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Actual)
Study Record Updates
Last Update Posted (Actual)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Additional Relevant MeSH Terms
Other Study ID Numbers
- Galectin -3 and pemphigus
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
Clinical Trials on Galectin-3 and Pemphigus
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General Practitioners Research InstituteG3 PharmaceuticalsEnrolling by invitation
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Asklepios Neurological Clinic Bad SalzhausenAsklepios Schlossberg Clinic Bad KoenigRecruiting
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argenxTerminatedPemphigus Vulgaris | Pemphigus FoliaceusUnited States, Germany, Italy, Australia, Bulgaria, China, France, Georgia, Greece, Hungary, India, Israel, Japan, Poland, Romania, Russian Federation, Serbia, Spain, Turkey, Ukraine, United Kingdom
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