Effects of Hydrolysed Porcine Proteins on Muscle Protein Synthesis and Appetite (SEPA)

The purpose of the study is to obtain a better understanding of how hydrolysed porcine proteins affect the human metabolism, including the effects on muscle protein synthesis, appetite and secretion of gastrointestinal hormones.

It is hypothesised that hydrolysed porcine proteins will stimulate muscle protein synthesis, affect appetite and the secretion of gastrointestinal hormones similar to hydrolysed whey protein.

Study Overview

• Status: Completed
• Conditions: Hunger; Satiety Response; Protein Synthesis
• Intervention / Treatment: Dietary supplement: Hydrolysed porcine protein from blood; Dietary supplement: Hydrolysed porcine protein from muscle; Dietary supplement: Hydrolysed whey protein

Detailed Description

Previous data show that proteins enhance satiety, decrease hunger and decrease energy intake. In addition, amino acids from dietary protein act as building blocks for de novo protein synthesis and consumption of dietary protein therefore stimulates protein synthesis. Proteins from different sources differ in amino acid composition and rate of absorption. Previously, studies on the effects of proteins on especially muscle protein synthesis has focused on milk proteins, whey and casein as animal sources, but other protein sources high in essential and branched-chain amino acids may also have beneficial effects on muscle protein synthesis and appetite. Thus, in this study the effects of two hydrolysed porcine proteins (one from porcine blood and one from porcine muscle) on muscle protein synthesis, appetite and secretion of gastrointestinal hormones will be compared with the effects of hydrolysed whey protein. These porcine proteins have never previously been tested and it is hypothesised that they may induce similar effects as hydrolysed whey protein due to the high contents of essential and branched-chain amino acids.

The study will be conducted as a randomised, 3-way, cross-over study. It consists of three visits separated by at least two weeks. It is expected that 18 normal-weight, young men will complete the study. They will be randomised to the order of the three proteins; hydrolysed porcine protein from blood (HPB), hydrolysed porcine protein from muscle (HPM) and hydrolysed whey protein (HW). At each visit the effects on muscle protein synthesis, appetite and secretion of gastrointestinal hormones will be measured/assessed. Muscle protein synthesis will be measured after consumption of a low (15 g) and a high (30 g) dose of protein and appetite and secretion of gastrointestinal hormones will be measured after the high protein dose (30 g). The effect on muscle protein synthesis will be measured by a flood-primed continuous infusion of labelled (Ring13C6) phenylalanine, muscle biopsies and blood samples. The effects on appetite will be assessed by visual analogue scales, an ad libitum meal and blood samples.

• Study Type: Interventional
• Enrollment (Actual): 21
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Denmark
  • Frederiksberg, Denmark, 1958
    • Department of Nutrition, Exercise and Sports

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 20 years to 40 years (Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• Healthy young men
• Normal weight (BMI 18.5-25 kg/m2)
• 22-40 years

Exclusion Criteria:

• Vegetarians
• Weight change >3kg within 2 months prior to start of the study
• Regular participation in cardio/strength training within 6 months prior to (and during) the study (>1 per wk)
• Use of alcohol >14 drinks/wk
• Drug abuse
• Smoking
• Regular consumption of protein supplements
• Use of over-the-counter or prescription medication that influences body weight, appetite or metabolism
• Diabetes Mellitus
• Dyslipidemia
• Diseases, which influence metabolism
• Donation of blood 3 months prior to start of (and during) the study
• Subjects with a hemoglobin value < 8 mol/L (measured at screening)
• Participation in other clinical studies 1 month prior to start of (and during) the study
• Subjects who are unable to give an informed consent.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Prevention
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: Double

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Hydrolysed porcine protein from blood; Dietary intervention with hydrolysed porcine protein from blood	Dietary supplement: Hydrolysed porcine protein from blood; Effects of hydrolysed porcine proteins on muscle protein synthesis and appetite
Experimental: Hydrolysed porcine protein from muscle; Dietary intervention with hydrolysed porcine protein from muscle	Dietary supplement: Hydrolysed porcine protein from muscle; Effects of hydrolysed porcine proteins on muscle protein synthesis and appetite
Experimental: Hydrolysed whey protein; Dietary intervention with hydrolysed whey protein	Dietary supplement: Hydrolysed whey protein; Effects of hydrolysed porcine proteins on muscle protein synthesis and appetite

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Change in Muscle protein synthesis (%FSR)	3 biopsies are taken at time 0, 150 min and 310 min at each visit
Change in Muscle protein synthesis (%FSR)	13 blood samples are taken over 310 min

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Visual analogue scales (10 cm)	Subjective appetite	Assessed at time 0, 160, 175, 200, 220, 250, 280, 310, 345 min at each of the three test days
Appetite - ad libitum energy intake	At 325 min an ad libitum meal of spaghetti bolognese is served, and the total energy intake is recorded	Measured once at time 325 min at each of the three test days
Postprandial response in glycemic control, plasma amino acids and appetite regulating hormones		Blood samples are taken at 0, 150, 180, 200, 220, 250, 280 and 310 min at each of the three test days

Other Outcome Measures

Outcome Measure	Measure Description	Time Frame
Urate in urine and blood	Explorative parameter	Blood samples are taken at 0, 20, 40, 60, 90, 120, 150, 180, 200, 220, 250, 280 and 310 min at each of the three test days. Urine is collected over 310 min at each test day
Muscle protein breakdown, gene expression	Explorative parameter	3 biopsies are taken at time 0, 150 min and 310 min. at each of the three test days
Metabolomics - urine and blood	Explorative parameter	Blood samples are taken at 0, 20, 40, 60, 90, 120, 150, 180, 200, 220, 250, 280 and 310 min at each of the three test days. Urine is collected over 310 min at each test day

Collaborators and Investigators

• Sponsor: University of Copenhagen
• Collaborators: Svineafgiftsfonden; DC Ingredients; Arla Food Ingredients Group P/S
• Investigators: Principal Investigator: Arne Astrup, DMSc, Department of Nutrition, Exercise and Sports, University of Copenhagen

Study record dates

Study Major Dates

• Study Start: July 1, 2015
• Primary Completion (Actual): July 1, 2016
• Study Completion (Actual): July 1, 2016

Study Registration Dates

• First Submitted: June 11, 2015
• First Submitted That Met QC Criteria: June 19, 2015
• First Posted (Estimate): June 22, 2015

Study Record Updates

• Last Update Posted (Estimate): January 11, 2017
• Last Update Submitted That Met QC Criteria: January 10, 2017
• Last Verified: January 1, 2017

More Information

Terms related to this study

• Keywords: appetite; muscle protein synthesis; gastrointestinal hormones; porcine protein
• Other Study ID Numbers: H-15003581

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: No