- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT03049631
Red Raspberry Polyphenols on Gut Microbiome (RRB2)
The Reciprocal Interactions Between Red Raspberry Polyphenols and Gut Microbiome Composition Affect Insulin Sensitivity
Study Overview
Status
Conditions
Intervention / Treatment
Detailed Description
The study will be performed as a single-blinded, randomized, crossover manner. Thirty subjects (20 insulin resistant subjects and 10 healthy subjects) will be randomly assigned into one of two sequences: sequence A (n=15) will be RRB then RRB+FOS (which means subject will consume only RRB (125 g/day(d) (equivalent to 1 cup fresh RRB)) with breakfast for 4 weeks, and then after a 4-week washout period, that subject will consume the RRB with FOS (125 g/d RRB and 8 g/d FOS) for 4 weeks); sequence B (n=15) will be RRB+FOS then RRB (which means subject will receive the RRB with FOS for 4 weeks, a 4-week washout, and then the RRB for another 4-week). At the beginning and end of each 4-week treatment, subjects will undergo a 6-h Study Day (4 study days in 12-week study duration) followed by a 1-h next morning follow-up visit.
Subjects will be required to meet several inclusion and exclusion criteria, which will be assessed through online survey and on-site clinic assessments, including questionnaires, blood analysis and anthropometric measures. Eligible subjects will be invited to participate in the study. Each subject will be asked to come for 1 Screening Visit, 4 dinner pick-ups (the day before each Study Day), 4 Study Days and 6 Weekly Meetings.
During the Screening Visit, subjects will read, sign and date a written Institutional Review Board approved Informed Consent Form prior to performing any study procedure. And then they will be assessed their qualification, instructed on the process for completing study questionnaires and counseled to restrict intake of colored plant foods rich in phytonutrients the 3 days prior to each Study Day. They will be asked to restrict alcohol intake, coffee/tea/ caffeinated beverage intake and moderate / vigorous physical activity and to drink plenty of water to maintain hydration in the 24 h prior to each Study Day. They will be instructed to come to the Clinical Nutrition Research Center (CNRC) the day before each Study Day to pick up their dinner meal and evening snack. Subjects will be asked to get at least 7 hours sleep and to come to the CNRC after a 10-h overnight fasting on each Study Day.
Each Study Day will require subjects to be in the clinic for ~7 h to complete all baseline and post challenge beverage testing procedures, including a 6 h study day and a 1 h next morning follow-up visit. Subjects will be evaluated for compliance with the protocol (diet, exercise, sleep, fasting), have their body weight and blood pressure measured, ingest a cup of red raspberry drink (250 g RRB (equivalent to 2 cups fresh RRB) and 64 g glucose) and their blood, urine and feces samples will be collected.
Study Type
Enrollment (Actual)
Phase
- Not Applicable
Contacts and Locations
Study Locations
-
-
Illinois
-
Chicago, Illinois, United States, 60616
- Clinical Nutrition Research Center
-
-
Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Genders Eligible for Study
Description
Inclusion Criteria:
- Non-smoking1 man or woman, 20-60 years of age, inclusive
- Group 1 must have a fasting blood glucose between 100-125 mg/dL and inulin ≥ 8 μIU/mL
- Group 2 will have a fasting blood glucose below 100 mg/dL and the homeostasis model assessment method of insulin resistance (HOMA-IR) [glucose (μmol/L) × insulin (μunits/μL)/22.5] value less than 1
- Judged to be in good health on the basis of the medical history
- Able to provide informed consent and comply with study procedures
- Able to comply to the study instructions (including dietary restrictions, consumption of study beverage, records of food diary and GI-tract questionnaire, sample collection procedure and study visit schedule)
- Able to maintain your usual physical activity pattern
- Able to abstain from alcohol consumption and avoid vigorous physical activity for 24 hours prior to and during study visit
Exclusion Criteria:
- Systolic blood pressure ≥160 mm Hg or diastolic blood pressure ≥100 mm Hg at screening visit.
- Major trauma or a surgical event within 2 months or longer depending on trauma or event and after consultation with PI.
- Weight change ≥4.5 kg (9.9 lbs) within 2 months
- Presence of chronic constipation, diarrhea or other chronic gastrointestinal complaint (e.g. irritable bowel syndrome)
- Had gastrointestinal barium opaque meal within 3 months
- History or presence of clinically important endocrine, cardiovascular (including, but not limited to, atherosclerotic disease, history of myocardial infarction, peripheral arterial disease, stroke), pulmonary, or biliary that, in the opinion of the investigator, could interfere with the interpretation of the study results
- History or presence of cancer in the prior 2 years, except for non-melanoma skin cancer
- History of extreme dietary habits, as judged by the investigator (e.g., Atkins diet, etc.)
- History of an eating disorder (e.g., anorexia nervosa, bulimia nervosa, or binge eating) diagnosed by a health professional
- Vegan or other extreme dietary regimens as judged by the investigator.
- Has a known intolerance or sensitivity to any ingredients in the study products
- Has used antibiotics within the previous 2 months
- Has used prebiotics, probiotics, or drugs active on gastrointestinal motility, or a laxative of any class within 1 month
- Has used medications known to influence carbohydrate metabolism, including, but not limited to adrenergic blockers, diuretics, hypoglycemic medications, and systemic corticosteroids within 2 weeks
- Female, who is pregnant, lactating or planning pregnancy during the study period
- Has participated in prebiotics or laxative trial within 3 months prior to enrollment or any other clinical trial within 1 month
- Donated blood within last 3 months
- Working overnight (e.g. 3rd shift)
- Excessive coffee and tea consumption (> 4 cups/day)
- Men and women who do excessive exercise regularly or athlete
- Men and women whom investigator is uncertain about subject's capability or willingness to comply with protocol requirement
Study Plan
How is the study designed?
Design Details
- Primary Purpose: Other
- Allocation: Randomized
- Interventional Model: Crossover Assignment
- Masking: Single
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
Active Comparator: Raspberry and fructooligosaccharide
Red raspberries (1 cup equivalent) with fructooligosaccharide (8 g)
|
Red raspberries (1 cup equivalent) with fructooligosaccharide (8 g)
|
Experimental: Raspberry
Red raspberries (1 cup equivalent)
|
Red raspberries (1 cup equivalent)
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Changes in gut microbiome composition as measured by 16S rRNA gene sequencing in 12-week intervention of active treatment vs Experimental
Time Frame: baseline, 4 weeks, 8 weeks and 12 weeks
|
gut microbiome composition changes
|
baseline, 4 weeks, 8 weeks and 12 weeks
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Changes in plasma red raspberry polyphenol metabolites in 12-week intervention of active treatment vs Experimental
Time Frame: baseline, 4 weeks, 8 weeks and 12 weeks
|
Plasma red raspberry polyphenol metabolites
|
baseline, 4 weeks, 8 weeks and 12 weeks
|
Changes in insulin sensitivity as measured by the Matsuda composite index of insulin sensitivity (MISI) in 12-week intervention of active treatment vs Experimental
Time Frame: baseline, 4 weeks, 8 weeks and 12 weeks
|
Plasma Insulin Sensitivity
|
baseline, 4 weeks, 8 weeks and 12 weeks
|
Changes in plasma glucose response in 12-week intervention of active treatment vs Experimental
Time Frame: baseline, 4 weeks, 8 weeks and 12 weeks
|
Plasma glucose response
|
baseline, 4 weeks, 8 weeks and 12 weeks
|
Changes in plasma insulin response in 12-week intervention of active treatment vs Experimental
Time Frame: baseline, 4 weeks, 8 weeks and 12 weeks
|
plasma insulin response
|
baseline, 4 weeks, 8 weeks and 12 weeks
|
Changes in urine creatinine concentration in 12-week intervention of active treatment vs Experimental
Time Frame: baseline, 4 weeks, 8 weeks and 12 weeks
|
urine creatinine concentration
|
baseline, 4 weeks, 8 weeks and 12 weeks
|
Changes in urine red raspberry polyphenol metabolites in 12-week intervention of active treatment vs Experimental
Time Frame: baseline, 4 weeks, 8 weeks and 12 weeks
|
urine red raspberry polyphenol metabolites
|
baseline, 4 weeks, 8 weeks and 12 weeks
|
Changes in breath hydrogen exhalation in 12-week intervention of active treatment vs Experimental
Time Frame: baseline, 4 weeks, 8 weeks and 12 weeks
|
breath hydrogen exhalation
|
baseline, 4 weeks, 8 weeks and 12 weeks
|
Changes in Gastrointestinal Health as measured by Gastrointestinal Tract Questionnaire in 12-week intervention of active treatment vs Experimental
Time Frame: baseline, 4 weeks, 8 weeks and 12 weeks
|
Gastrointestinal Health
|
baseline, 4 weeks, 8 weeks and 12 weeks
|
Changes in blood pressure in 12-week intervention of active treatment vs Experimental
Time Frame: baseline, 4 weeks, 8 weeks and 12 weeks
|
blood pressure
|
baseline, 4 weeks, 8 weeks and 12 weeks
|
Other Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Changes in body weight in 12-week intervention of active treatment vs Experimental
Time Frame: baseline, 4 weeks, 8 weeks and 12 weeks
|
body weight
|
baseline, 4 weeks, 8 weeks and 12 weeks
|
Changes in waist circumference in 12-week intervention of active treatment vs Experimental
Time Frame: baseline, 4 weeks, 8 weeks and 12 weeks
|
waist circumference
|
baseline, 4 weeks, 8 weeks and 12 weeks
|
Changes in plasma triglycerides in 12-week intervention of active treatment vs Experimental
Time Frame: baseline, 4 weeks, 8 weeks and 12 weeks
|
plasma triglycerides
|
baseline, 4 weeks, 8 weeks and 12 weeks
|
Changes in plasma LDL cholesterol in 12-week intervention of active treatment vs Experimental
Time Frame: baseline, 4 weeks, 8 weeks and 12 weeks
|
plasma LDL cholesterol
|
baseline, 4 weeks, 8 weeks and 12 weeks
|
Changes in plasma HDL cholesterol in 12-week intervention of active treatment vs Experimental
Time Frame: baseline, 4 weeks, 8 weeks and 12 weeks
|
plasma HDL cholesterol
|
baseline, 4 weeks, 8 weeks and 12 weeks
|
Collaborators and Investigators
Publications and helpful links
Study record dates
Study Major Dates
Study Start (Actual)
Primary Completion (Actual)
Study Completion (Actual)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Actual)
Study Record Updates
Last Update Posted (Actual)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Keywords
Additional Relevant MeSH Terms
Other Study ID Numbers
- IRB2016-136
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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