Allogeneic γδ T Cells Immunotherapy in r/r Non-Hodgkin's Lymphoma (NHL) or Peripheral T Cell Lymphomas (PTCL) Patients

The Safety and Efficacy Assessment of Ex-Vivo Expanded Allogeneic γδT Cells Immunotherapy in Patients With Relapsed or Refractory Non-Hodgkin's Lymphoma (NHL) and Peripheral T Cell Lymphomas (PTCL)

This study aims to evaluate the safety, tolerability and efficacy of ex-vivo expanded allogeneic γδT cells obtained from a blood-related donor of patients with relapsed or refractory B cell non-Hodgkin's lymphoma (B-NHL) or peripheral T cell lymphoma (PTCL) expect for γδT lymphoma.

Study Overview

• Status: Recruiting
• Conditions: Non-Hodgkin's Lymphoma (NHL); Peripheral T Cell Lymphoma (PTCL)
• Intervention / Treatment: Biological: Ex-vivo expanded allogeneic γδT cells

Detailed Description

This study is a single-center, non-randomized, open label, no control, prospective clinical trial to evaluate the safety, tolerability and efficacy of ex-vivo expanded allogeneic γδT cells from a blood-related donor of NHL or PTCL patients(except for γδT lymphoma). This study will include the following sequential phases: sign informed consent, γδT cell pre-culture, screening and registration to the trial, apheresis, γδT cell preparation, pre-treatment for lymphodepleting chemotherapy (selectable plan), treatments and follow-ups. The study will evaluate the safety and efficacy of the ex-vivo expanded allogeneic γδT cells in patients with relapsed or refractory non-Hodgkin's lymphoma (NHL) or peripheral T cell lymphoma (PTCL) expect for γδT lymphoma.

• Study Type: Interventional
• Enrollment (Anticipated): 10
• Phase: Early Phase 1

Contacts and Locations

• Study Contact: Name: Dehui Zou, MD; Phone Number: 86-022-23909283; Email: zoudehui@ihcams.ac.cn

Study Locations

• China
  • Tianjin
    • Tianjin, Tianjin, China, 300020
      • Recruiting
      • Institute of Hematology & Blood Disease Hospital
      • Contact: Shuhua Yi, MD; Phone Number: 86-022-23909106; Email: yishuhua@ihcams.ac.cn

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Patient Inclusion Criteria:

  1. Patients should sign informed consent form voluntarily before the trail and comply with the requirements of this study.
  2. Age≥18 years old, gender unlimited.
  3. Patients whose relatives are willing to donate PBMCs voluntarily.
  4. Patients with relapsed or refractory B cell non-Hodgkin's lymphoma (B-NHL) or peripheral T cell lymphoma (PTCL) expect for γδT lymphoma.
  5. Patients had an evaluable imaging lesion of at least greater than 1.5 cm.
  6. Eastern Cooperative Oncology Group (ECOG) Performance score≤2.

  7. Adequate bone marrow function:

     • Absolute neutrophil count (ANC) >1000/mm3;
     • Absolute lymphocyte count (ALC)≥300/mm3;
     • PLT≥50,000/mm3;
     • Hb >8.0g/dl.

  8. Adequate organ function:

     • Alanine aminotransferase (ALT)≤3 times the upper limit of normal (ULN);
     • Aspartate aminotransferase (AST)≤3 times ULN
     • TBIL≤1.5 times ULN (Gilbert syndrome patients TBIL≤3 times ULN and DBIL≤1.5 times ULN)
     • Scr≤1.5 times ULN or CCR≥60 mL/min/1.73m3 Note: apart from tumor infiltrated liver dysfunction.
  9. Male and female patients of reproductive potential must agree to use birth control during the study and for at least 12 weeks post study.

• Donor Inclusion Criteria:

  1. Sign informed consent form.
  2. Age 18 years up to the age of 60 (≤60), gender unlimited.
  3. Relatives of patients (unrestricted to blood relationship).
  4. Apheresis available.
  5. PLT≥100×109/L with normal APTT or PT.

Exclusion Criteria:

• Patient Exclusion Criteria:

  1. Patients with other available treatment drugs or treatment options.
  2. Patients with history of allogeneic hematopoietic stem cell transplantation (Allo-HSCT).
  3. Active central nervous system (CNS) lymphoma.

  4. Patients receiving chemotherapy within 1 week prior to γδT cell transfusion, with the following exceptions:

     • Pretreatment chemotherapy prescribed by the protocol
     • Other exploratory combined medications
  5. Systemic glucocorticoid treatment 72h prior to γδT cell transfusion (apart from physiological replacement dosage).
  6. Biphosphonates were used 2 months prior to γδT cell transfusion.
  7. Patients with systemic vasculitis, or with active or uncontrolled autoimmune diseases, as well as primary or secondary immunodeficiency diseases.
  8. Active HBV, HCV, HIV, TP, CMV or EBV infection.
  9. Major surgery that was evaluated by the investigator as unsuitable for inclusion within 4 weeks prior to screening.
  10. Patients with malignant tumors, apart from those who has been cured for at least 2 years.

  11. Patient's cardiac function meets any of the following conditions:

      • Left ventricular ejection fraction (LVEF)≤45%
      • Class III or IV heart failure according to the NYHA Heart Failure Classifications
      • QTcB>450 msec
      • Other cardiac disease that investigators judge is not suitable for enrollment
  12. History of epilepsy or other active central nervous system disorders.
  13. Inoculated live vaccine within 6 weeks before screening.
  14. Uncontrolled serious active infection (such as sepsis, bacteremia and fungemia).
  15. Life expectancy < 3 months
  16. Participated in any other interventional clinical trial within 3 months prior to γδT cell transfusion.
  17. Any situation that investigators believe the risk of the subjects is increased or results of the trial are disturbed: patients with any serious acute or chronic physical or mental illness, or laboratory abnormalities.

• Donor Exclusion Criteria:

  1. History of any severe clinical diseases or other severe organic diseases, including any history of clinically significant systematic diseases such as cardiovascular, urinary, circulatory, respiratory, neurological, psychiatric, digestive and endocrine diseases. History of high blood pressure or systolic pressure>140 mmHg, diastolic pressure>90 mmHg in screening stage. Any situation that investigators believe is clinically significant or with other severe diseases unsuitable of apheresis.
  2. Arterial thrombosis or venous thrombosis history 12 months prior to the trial or hemorrhagic tendency or history 2 months prior to the trial; oral administration of anticoagulation drugs (e. g. aspirin and warfarin).
  3. Active or history of autoimmune diseases including but not restricted to SLE, psoriasis, RA, IBD and HT. Apart from hypothyrosis which can be controlled by hormone replacement therapy, skin diseases without systemic therapy and celiac disease which is fully controlled.
  4. HIV-Ab, TP-Ab, HCV-Ab, HBsAg, HBeAg, HBeAb or HBcAb positive.
  5. Any symptom, sign or laboratory examination abnormality suggesting acute or subacute infection (e.g. fever, cough, urinary irritation, skin infectious wound).
  6. Female who are pregnant or cannot stop lactating.
  7. Those who cannot communicate with medical staff due to mental illness or language disabilities.
  8. Other unsuitable conditions that investigators believe unsuitable for the donation.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: N/A
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

1

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Allogeneic γδT cell immunotherapy; Patients will receive 2 cycles of ex-vivo expanded allogeneic γδT cells treatments, at 14 days' intervals, each cycle has 2 infusions. Ex-vivo expanded γδT cells are transfused to patients in a dosage escalated manner (Dose escalation, 1×107, 3×107, 9×107 per kg of body weight).	Biological: Ex-vivo expanded allogeneic γδT cells; Cells will be extracted from a healthy donor by apheresis, followed by ex-vivo expansion and activation. The ex-vivo expanded γδT cells from donors will be adoptively transfused.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Safety evaluation: Incidence of Adverse events (AEs)	Therapy-related adverse events will be recorded and assessed according to the National Cancer Institute's Common Terminology Criteria for Adverse Events (CTCAE, Version 5.0).	2 years post γδT cells infusion
Safety evaluation: Dose limited toxicity (DLTs)	The incidence of DLTs will be recorded and assessed.	28 days
Safety evaluation: Maximum-tolerated dose (MTD)	MTD or clinical recommended dose will be recorded and evaluated.	28 days

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Efficacy evaluation：Overall response rate (ORR)	ORR is defined as the incidence of either a CR or a partial response (PR) per the Lugano Classification as determined by study investigators.	2 years post γδT cells infusion
Efficacy evaluation：Disease control rate (DCR)	DCR is defined as the incidence of either a CR, a partial response (PR) or stable disease (SD) per the Lugano Classification as determined by study investigators.	2 years post γδT cells infusion
Efficacy evaluation：Duration of remission (DOR)	DOR is defined only for participants who experience an objective response after γδT cells infusion and is the time from the first objective response to disease progression or death from any cause.	2 years post γδT cells infusion
Efficacy evaluation：Progression free survival (PFS)	PFS is defined as the time from the γδT cells infusion date to the date of disease progression or death from any cause.	2 years post γδT cells infusion
Efficacy evaluation：Overall survival (OS)	OS is defined as the time from γδT cells infusion to the date of death from any cause.	2 years post γδT cells infusion
Pharmacokinetics (PK) evaluation :γδT cells in peripheral blood	Number of γδT cells in peripheral blood will be assessed by flow cytometry.	2 years post γδT cells infusion

Collaborators and Investigators

• Sponsor: Institute of Hematology & Blood Diseases Hospital
• Collaborators: Cancer Institute and Hospital, Chinese Academy of Medical Sciences; Beijing GD Initiative Cell Therapy Technology Co., Ltd.
• Investigators: Study Director: Jianmin Zhang, PhD, Cancer Institute and Hospital, Chinese Academy of Medical Sciences

Study record dates

Study Major Dates

• Study Start (Actual): December 31, 2020
• Primary Completion (Anticipated): December 25, 2021
• Study Completion (Anticipated): December 25, 2023

Study Registration Dates

• First Submitted: December 23, 2020
• First Submitted That Met QC Criteria: January 5, 2021
• First Posted (Actual): January 6, 2021

Study Record Updates

• Last Update Posted (Actual): January 6, 2021
• Last Update Submitted That Met QC Criteria: January 5, 2021
• Last Verified: January 1, 2021

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Immune System Diseases; Neoplasms by Histologic Type; Neoplasms; Lymphoproliferative Disorders; Lymphatic Diseases; Immunoproliferative Disorders; Lymphoma; Lymphoma, Non-Hodgkin; Lymphoma, T-Cell; Lymphoma, T-Cell, Peripheral
• Other Study ID Numbers: IIT2020028-EC-3

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: No

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No