- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT05072665
Fast Allergy Sensitivity Test (FAST)
EFFECT OF FOOD ALLERGENS ON DUODENAL MUCOSAUS BY CONFOCAL ENDOSCOPIC IMAGING Cellvizio
Study Overview
Status
Conditions
Intervention / Treatment
Detailed Description
In 2014, Fritscher-Ravens et al., Described for the first time the value of endomicroscopy in the diagnosis of food allergies by observing the reaction of the duodenal mucosa to food allergens in patients with IBS. They assessed the structural and functional changes that were occurring in the intestinal lining in vivo and noted the following changes at the cellular level:
- The layers of epithelial cells break down and are removed forming voids and inducing an immediate increase in the fluid permeability of the duodenal mucosa. As a result, the contrast agent penetrates the lumen, widening the space between the villi and changing the appearance from black to white.
- These changes were usually seen between 2 min and 4 min after exposure to the allergen.
This procedure, called FAST (Food Allergy Sensitivity Test), has since been used routinely in certain centers in Germany. However, few studies have been published that have shown the reproducibility of these results as well as the performance of this new test. The study that the investigators propose to conduct is a prospective multicenter pilot study conducted in the Trocadero Clinic
1. Pre-test consultation The patients will first be seen by a nutritionist. During this consultation, eligible patients will be offered, in addition to endoscopy performed in current practice, the possibility of performing the FAST test during endoscopy. Up to 7 days before the operation, a questionnaire allowing the assessment of the severity of symptoms (IBS-SSS) will be provided to the patient who has agreed to participate in the study. The answers to the questionnaire will be used as a reference to assess the evolution of symptoms if a food exclusion diet were to be put in place following the procedure. Finally, two to three days before the FAST test, the patient will be asked to follow an exclusion diet and consume only hypoallergenic nutritional foods: rice, potatoes, olive oil, salt.
2 Carrying out the test Before the start of the test, a standard high-end endoscopy will be performed to look for any signs of structural mucosal abnormalities that suggest gastrointestinal disease. In the event of a mucosal abnormality observed on endoscopy, the CLE FAST procedure will be canceled. In the case of mild reflux disease, the test may be done.
Prior to the first challenge, 2.5 ml of 10% fluorescein (SERB) will be administered to the patient intravenously.
Before any challenge and after the fluorescein injection, to establish a comparison image, a first acquisition with the endomicroscopy system of the duodenal mucosa will be carried out on a minimum of 4 sites (approximately 20 seconds each) to verify the integrity of the mucosa.
During the acquisition of the reference sequences and during all subsequent acquisitions, areas of the mucosa that show bleeding and other visible signs of inflammation will not be imaged in order to avoid false positives.
Once the reference images have been acquired, a first food allergen will be applied to the duodenal mucosa starting with the most distal part of the duodenum. After 2 minutes following the application of the allergen, observation using the endomicroscopy system can begin by applying the GastroFlex ™ UHD probe to the duodenal mucosa where the allergen has been projected. . Observation will last up to 3 minutes per site observed. If no reaction is observed, the same manipulation will be carried out using a new allergen. If the observed reaction is positive, the test will be stopped.
The test can be performed using up to 5 different allergens as long as the total time after fluorescein injection does not exceed 30min.
Study Type
Enrollment (Actual)
Phase
- Not Applicable
Contacts and Locations
Study Contact
- Name: bouchra BENKESSOU, Project Manager
- Phone Number: 0764486016
- Email: bbenkessou@hexagone-sante-paris.fr
Study Locations
-
-
-
Paris, France, 75016
- Clinique du Trocadero
-
-
Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Description
Inclusion Criteria:
- Patients over 18 years old
- Diagnosis of irritable bowel syndrome according to the ROME IV classification
- Negative blood test: determination of IgE; anti-transglutaminase IgA assay (celiac disease)
- Symptoms potentially related to a food allergy
- Sufficient cooperation and understanding to comply with the requirements of the study with a post-Cellvizio medical examination at 2 weeks, 2 months and 6 months
Exclusion Criteria:
- Patients under 18
- Patient benefiting from a legal protection measure (curatorship, guardianship, etc.)
- Pregnant or breastfeeding woman
- Known allergy to fluorescein
- Digestive cancer (stomach cancer colon rectal cancer)
Study Plan
How is the study designed?
Design Details
- Primary Purpose: Diagnostic
- Allocation: N/A
- Interventional Model: Single Group Assignment
- Masking: None (Open Label)
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
Experimental: PATIENT WITH Irritable Bowel Syndrome
2.5 ml of 10% fluorescein (SERB) will be administered to the patient intravenously. after the fluorescein injection. A first food allergen will be applied to the duodenal mucosa starting with the most distal part of the duodenum. After 2 minutes following the application of the allergen, observation using the endomicroscopy system can begin by applying the GastroFlex ™ UHD probe to the duodenal mucosa where the allergen has been projected. . Observation will last up to 3 minutes per site observed. If no reaction is observed, the same manipulation will be carried out using a new allergen. If the observed reaction is positive, the test will be stopped |
HIGH ECHOENDOSCOPY WITH THE FAST PROCEDURE
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Observation, at the time of the endomicroscopy procedure, of ruptures / leaks in the mucous membranes and intercellular extravasation of plasma fluid labeled with fluorescein in the intercellular space
Time Frame: day 1
|
day 1
|
|
SCORE ROME IV
Time Frame: DAYS 28
|
QUESTIONNARY
|
DAYS 28
|
SCORE ROME IV
Time Frame: 2 months
|
QUESTIONNARY
|
2 months
|
SCORE ROME IV
Time Frame: 6 months
|
QUESTIONNARY
|
6 months
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
SCORE OBS-SSS
Time Frame: days 28
|
QUESTIONNARY
|
days 28
|
SCORE OBS-SSS
Time Frame: 2months
|
QUESTIONNARY
|
2months
|
SCORE OBS-SSS
Time Frame: 6 months
|
QUESTIONNARY
|
6 months
|
RESULT OH THE DIET
Time Frame: 6 MONTHS
|
consultation
|
6 MONTHS
|
Collaborators and Investigators
Sponsor
Study record dates
Study Major Dates
Study Start (Actual)
Primary Completion (Actual)
Study Completion (Actual)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Actual)
Study Record Updates
Last Update Posted (Actual)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Additional Relevant MeSH Terms
Other Study ID Numbers
- 2021-A01267-34
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
Clinical Trials on Food Allergy
-
Vilnius UniversityEnrolling by invitationFood Allergy in Children | Food Allergy in InfantsLithuania
-
Vilnius UniversityEnrolling by invitationFood Allergy in Children | Food Allergy in InfantsLithuania
-
Assistance Publique Hopitaux De MarseilleUnknown
-
Chinese University of Hong KongRecruiting
-
King's College LondonKing's College Hospital NHS Trust; University College London Hospitals; Cambridge... and other collaboratorsRecruitingEgg Allergy | Food Allergy | Food Allergy in Children | Milk Allergy | Food Allergy in Infants | Nut Allergy | Food Allergen SensitisationUnited Kingdom
-
King's College LondonGuy's and St Thomas' NHS Foundation TrustUnknownEgg Allergy | Food Allergy | Food Allergy in Children | Milk Allergy | Food Allergy in Infants | Nut Allergy | Food Allergen SensitisationUnited Kingdom
-
Stanford UniversityRecruitingFood Allergy | Food Allergy in ChildrenUnited States
-
Rhode Island HospitalCompletedFood Allergy in Children
-
University Hospital, MontpellierUnknownFood Allergy Suspected | Food Allergy ProvedFrance
-
Rhode Island HospitalVirtually Better, Inc.CompletedFood Allergy in Children
Clinical Trials on HIGH ECHOENDOSCOPY WITH THE FAST PROCEDURE
-
Bahçeşehir UniversityCompletedPain, Postoperative | Diabetes | Nausea | Obesity, Morbid | Bariatric Surgery Candidate | Postoperative Nausea and Vomiting | Postoperative Nausea | Post Procedural Discharge | DVT | Obesity Adult Onset | ERAS | Obesity Associated Disorder | AcheTurkey
-
Treace Medical Concepts, Inc.RecruitingHallux Valgus | Metatarsus AdductusUnited States
-
James F. Holmes, MD, MPHEunice Kennedy Shriver National Institute of Child Health and Human Development... and other collaboratorsRecruitingWounds and Injuries | Abdomen, Acute | Abdominal Injury | Blunt Trauma to Abdomen | Abdomen InjuryUnited States
-
Assistance Publique Hopitaux De MarseilleUnknownGynecological SurgeryFrance
-
Silesian Centre for Heart DiseasesMedical University of Silesia; Biostat Sp. z o.o.; Leszek Giec Upper-Silesian...RecruitingDepression | Cognitive Impairment | Atrial Fibrillation | Silent StrokePoland
-
Second Affiliated Hospital, School of Medicine,...The Second Affiliated Hospital of Jiaxing University; Changxing People's Hospital and other collaboratorsRecruiting
-
Frederic PRAT, MD, PhDRecruitingMalignant Biliary StenosisFrance
-
Aalborg UniversityAalborg University HospitalRecruiting
-
Istituto Clinico HumanitasRecruiting
-
Carl Zeiss Meditec, Inc.Active, not recruitingAstigmatism | MyopiaUnited States