Omega-3 Fatty Acid Supplementation and Fractional Iron Absorption in Obese South African Women

Effects of Omega-3 Fatty Acid Supplementation on Fractional Iron Absorption in South African Women Living With Obesity: A Stable Iron Isotope Study

In South Africa the prevalence of obesity in women of reproductive age is high; these women also have a high risk for iron deficiency (ID). Obesity is associated with low-grade systemic inflammation, which was shown to increase the expression of hepcidin, leading to a reduction in duodenal iron absorption. Thus, alleviating the sub-clinical inflammation associated with obesity could improve iron absorption and status. Supplementation with n-3 long-chain polyunsaturated fatty acids (LCPUFA) has been shown to reduce inflammation in obese individuals. A stable iron isotope study will be performed to investigate the effect of n-3 LCPUFA supplementation on fractional iron absorption in obese South African women.

Study Overview

• Status: Completed
• Conditions: Obesity
• Intervention / Treatment: Dietary supplement: Omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA)

Detailed Description

Iron deficiency (ID) and iron-deficiency anaemia (IDA) remain a major public health problem in low- and middle-income countries (LMICs). Women of reproductive age are at high risk of developing ID due to loss of iron through menstruation. Furthermore, inadequate iron status at conception and during pregnancy can have detrimental effects on the developing offspring. In addition to ID, the prevalence of obesity is increasing globally, including in LMICs. South Africa is a country with a high prevalence of obesity, especially in women of reproductive age (36 %), and a persistent burden of ID despite a mandatory maize meal and wheat flour fortification programme. Obesity is a condition associated with low-grade systemic inflammation. Inflammation increases the expression of hepcidin, leading to a reduction in duodenal iron absorption. Previous studies have shown that iron absorption differs between normal weight and obese individuals, and that the enhancing effect of ascorbic acid on iron absorption is lower in obese subjects. A possible explanation is the different sites of action of ascorbic acid and hepcidin on the enterocytes: Ascorbic acid enhances iron transport into enterocytes at the luminal side (via divalent metal transporter-1), while hepcidin reduces iron absorption by ferroportin inhibition at the basolateral membrane of the enterocyte. Thus, in obese individuals, an intervention at the basolateral membrane of the enterocyte will be needed. Supplementation with n-3 long-chain polyunsaturated fatty acids (LCPUFA) has been shown to exert cardiometabolic benefits, and to reduce inflammation in obese individuals.

The aim of this study is to investigate the effect of n-3 LCPUFA supplementation on fractional iron absorption in obese South African women of reproductive age. As a secondary objective, this study will determine association between omega-3 fatty acid status, inflammation and iron status in obese South African women. The hypothesises are: 1) fractional iron absorption will increase following n-3 LCPUFA supplementation; 2) iron absorption will further increase in the presence of ascorbic acid following n-3 LCPUFA supplementation.

After screening, apparently healthy, non-anaemic, obese South African women of African descent, aged 18 to 35 years, with low-grade inflammation and a low n-3 LCPUFA status, will receive daily oral fish oil (2.1 g of n-3 LCPUFA) capsules for three months. Iron status indices, inflammatory markers, hepcidin and omega-3 fatty acid index will be assessed at screening. The same variables will be emasured again at baseline and endpoint, in addition to erythrocyte total phospholipid fatty acid composition. Iron absorption will be determined from test meals with and without ascorbic acid using the oral stable isotope method, before (baseline) and after (endpoint) supplementation with n-3 LCPUFA.

• Study Type: Interventional
• Enrollment (Actual): 33
• Phase: Not Applicable

Contacts and Locations

• Study Contact: Name: Prof. Marius Smuts, PhD; Phone Number: +27 18 299 2086; Email: Marius.Smuts@nwu.ac.za

Study Locations

• South Africa
  • Potchefstroom, South Africa
    • Centre of Excellence for Nutrition, North-West University

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 35 years (Adult)
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• Self-classified women from African descent and of reproductive age (18 - 35 years)
• BMI ≥ 28kg/m2 (indicating obesity)
• Midly- or non-anaemic (Hb ≥11 g/dl)
• Low n-3 LCPUFA status (RBC omega-3 index < 6%)
• Low grade systemic inflammation (HS-CRP ≥ 2 mg/l but ≤ 20 mg/l)
• Be able to read and understand English

Exclusion Criteria:

• Treated chronic disease or gastrointestinal disorders
• Regular use of medication (except oral contraceptives, others after approval by the investigator) and women receiving treatment for high blood pressure
• Current consumption of iron or n-3 LCPUFA or ascorbic acid-containing supplements other than the supplements provided (Participants will be asked to discontinue use three weeks prior to enrolment)
• Subject on a weight loss diet or planning to start a weight loss diet during the duration of the study
• Pregnancy or lactation
• Subjects who cannot be expected to comply with the study protocol
• Subjects who are smoking
• Difficulty drawing blood due to poor quality veins
• Individuals that have a fear of needles or suffer from vaso-vagal episodes when exposed to blood
• Subjects with fish allergies
• Participants who plan to start or stop the use of contraceptives in the following 4 months.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Prevention
• Allocation: N/A
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

1

Arms and Interventions

Participant Group / Arm

Intervention / Treatment

Experimental: Experimental; Participants will receive three fish oil capsules daily for 14 weeks, and fractional iron absorption from test meals provided without and with ascorbic acid will be determined at baseline and endpoint.

Dietary supplement: Omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA); 3 three fish oil capsules providing 2.1 g n-3 PUFA; 1.27 g Docosahexanoic acid (DHA) and 0.86 g Eicosapentanoic acid (EPA) capsules. The absorption study test meals will consist of white bread with butter and honey, 6mg of labeled iron as ferrous sulfate and 300 g of distilled water. The meal will be given on two days without and with ascorbic acid. The test meals without ascorbic acid will contain 6 mg 57Fe as ferrous sulfate whereas the meals containing ascorbic acid will contain 6 mg 58Fe as ferrous sulfate.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Fractional iron absorption (%)	Fractional iron absorption will be assessed from labeled meals consumed without and with ascorbic acid, before and after intervention with n-3 LCPUFA.	Change between baseline and 3 months (days 17 and 118)

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Haemoglobin (g/dL)	Haemoglobin will be measured in whole blood to determine presence of anaemia.	Screening, days 1 and 102
Ferritin (µg/L )	Ferritin will be measured in plasma to determine the presence of iron deficiency.	Screening, days 1 and 102
Soluble transferrin receptor (mg/L)	Soluble transferrin receptor will be measured in plasma to determine the presence of iron deficiency.	Screening, days 1 and 102
Hepcidin concentration	Hepcidin, a major regulator of iron absorption and influenced by inflammation, will be measured in plasma.	Screening, days 1 and 102
C-reactive protein (mg/L)	C-reactive protein will be measured in plasma to determine the presence of acute inflammation.	Screening, days 1 and 102
Alpha-1-acid glycoprotein (g/L)	Alpha-1-acid glycoprotein will be measured in plasma to determine the presence of chronic inflammation.	Screening, days 1 and 102
Intestinal fatty acid-binding protein	Intesinal fatty acid-binding protein will be measured in plasma as a marker of intestinal damage.	Screening
Omega-3 fatty acid index (%)	Omega-3 fatty acid index, a measure of eicosapentanoic and docosahexanic acids will be measued in red blood cells.	Screening
Cytokines (pg/ml)	Cytokines: Interleukin (IL) IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, Interferon gamma and Tumor necrosis factor-beta will be measured in plasma as markers of inflammation.	Screening, days 1 and 102
Erythrocyte fatty acid composition	Fatty acid composition and status will be determined in erythrocyte phospholipids.	Days 1 and 102
Lipid mediators (mol/mL or pmol/mL)	The following lipid mediators will be measured in plasma: 17-Hydroxydocosahexanoic acid, 5-, 8-, 11-, 12-, 15- and 18-Hydroxyeicosapentanoic acid (HEPE); 5-, 8-, 11-, 12- and 15-Hydroxyeicosatetraenoic acids (HETE); Prostaglandinds E2, E3 and D2; Protectin D1 and Resolvin D1 and E1.	Days 1 and 102
Genetic variants in genes associated with fatty acid metabolism	Single nucleotide polymorphism (SNP) analyses of genes involved in fatty acid metabolism will be measured from stored buffy coat.	Days 1 and 102

Collaborators and Investigators

• Sponsor: Swiss Federal Institute of Technology
• Collaborators: North-West University, South Africa

Study record dates

Study Major Dates

• Study Start (Actual): March 7, 2022
• Primary Completion (Actual): March 10, 2023
• Study Completion (Actual): September 30, 2023

Study Registration Dates

• First Submitted: January 6, 2022
• First Submitted That Met QC Criteria: February 1, 2022
• First Posted (Actual): February 2, 2022

Study Record Updates

• Last Update Posted (Actual): November 22, 2023
• Last Update Submitted That Met QC Criteria: November 21, 2023
• Last Verified: November 1, 2023

More Information

Terms related to this study

• Keywords: Inflammation; Omega-3 fatty acids; Fractional iron absorption
• Additional Relevant MeSH Terms: Overnutrition; Nutrition Disorders; Overweight; Body Weight; Obesity
• Other Study ID Numbers: FeMEGA-3

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No