- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT05745636
Broccoli Seed Extract and Skin Health
Influence of TrueBroc® Broccoli Seed Extract Supplementation on Skin Health
Study Overview
Status
Conditions
Intervention / Treatment
Detailed Description
The primary objective of this study is to examine the influence of 4-weeks ingestion of TrueBroc® with mustard seed powder on improving skin health by evaluating skin physiological and biochemical parameters. TrueBroc® Glucoraphanin-Rich Broccoli Seed Extract (TrueBroc®) is a hot water extract of Brassica oleracea var. Italica Plenck (Brassicaceae) seeds. TrueBroc® contains high levels of glucoraphanin, a glucosinolate prominently found in the cruciferous vegetables. This study will use supplements containing 770 mg Broccoli Seed Extract (BSE) with 290 mg mustard seed powder (MSP) and 200 mg vitamin C. Limited data indicate that this mixture enhances glucoraphanin bioavailability. The consumption of the phytochemical glucoraphanin confers a number of benefits including skin health.
Hypothesis: That 4-weeks ingestion of TrueBroc® with MSP compared to placebo will improve skin health.
Study Procedures This study will incorporate a randomized, double-blinded, placebo-controlled, crossover design with 4-week supplementation and a 2-week washout period. Skin measurements and tape stripping samples will be collected pre- and post-4-week supplementation periods. Skin tape stripping samples will be analyzed for oxinflammatory-related outcomes. Blood and 24-hour urine samples will also be collected pre- and post-4-week supplementation periods. These samples will be aliquoted and stored in a freezer for potential analysis of targeted outcomes pending additional funding.
Visit #1: Screening and Consent. Subjects will come to the Human Performance Lab at assigned times. The COVID-19 screening participant form will be filled in by the participant to verify the absence of symptoms or exposure.This visit will begin with a review of the consent form, and the inclusion and exclusion criteria. Height, body weight, body composition (seca BIA), and blood pressure will be measured. Subjects will be given 24-hour collection kits with instructions to collect all urine in the provided jug during the day before visit #2.
Visit #2: Return urine bottles. Provide blood sample. Skin health measurements. Start 4-weeks supplementation.
Subjects will come to the Human Performance Lab at assigned times in an overnight fasted state (no intake of food or beverages except for water during at least the previous 9 hours). Subjects will drop off the urine bottles and provide an overnight fasted blood sample. Skin health measurements will involve having probes and tapes applied to the surface of the skin to assess various parameters of skin health. Photos of skin areas will be taken. These skin health measurements will take about 15 to 30 minutes. A 4-week supply of capsules will be provided in supplement trays or bottles, and an additional 24-hour urine collection kit will be given to each subject. Subjects will be contacted weekly by the Research Manager to ensure adherence to the supplementation regimen (2 capsules per day, 4 weeks). Visit #3: Repeat visit #2 test procedures. Subjects will come to the Human Performance Lab at assigned times in an overnight fasted state after 4 weeks of supplementation. Subjects will drop off the urine bottles and provide a blood sample. Body composition and blood pressure measurements will be repeated. Skin health measurements will be repeated. An additional 24-hour urine collection kit will be given to each subject. Subjects will be instructed to return to the lab after the 2-week washout period.
Visits #4 and #5: Repeated procedures from visits #2 and #3. Subjects will crossover to the opposite trial supplement and repeat all study procedures.
Study Type
Enrollment (Actual)
Phase
- Not Applicable
Contacts and Locations
Study Contact
- Name: David C Nieman, DrPH
- Phone Number: 8287730056
- Email: niemandc@appstate.edu
Study Locations
-
-
North Carolina
-
Kannapolis, North Carolina, United States, 28081
- Appalachian State University Human Performance Lab, North Carolina Research Campus
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Principal Investigator:
- David Nieman, DrPH
-
Contact:
- David C Nieman, DrPH
- Phone Number: 828-773-0056
- Email: niemandc@appstate.edu
-
Contact:
- Camila A Olson, PhD
- Phone Number: 7042505352
- Email: olsonca1@appstate.edu
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-
Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Description
Inclusion Criteria:
- Both male and female subjects will be included, ages 25 to 57 years.
- Non-smoker and free of any medical conditions that might affect study measurements.
- Body mass index (BMI) of 20 - 29.9 kg/m2 (not obese).
- Willingness to adhere to dietary restrictions: refrain from consuming cruciferous vegetables (or related condiments and supplements) such as broccoli, cauliflower, cabbage, kale, bok choy, arugula, Brussels sprouts, collards, watercress, radishes, prepared mustard condiment, mustard greens, horseradish, wasabi, and mayonnaise for 3 days before and for the duration of the study.
- Willing to collect all urine for 24 hours and provide four blood samples on four separate occasions.
Exclusion Criteria:
- Diarrhea or oral antibiotic intake within the last 4 weeks.
- Use of proton pump inhibitors (PPIs), antacids or other medications that influence stomach acidity.
- History of malabsorption or GI tract disorders or GI surgeries (i.e. lapband, gastric bypass, etc.).
- History of allergic reactions or intolerance to any of the ingredients contained in the test supplements (e.g. broccoli, broccoli seeds, mustard seeds, vitamin C).
- Currently pregnant, planning to become pregnant, or breastfeeding.
- Any other condition which in the Investigator's opinion may adversely affect the subject's ability to complete the study or its measures or which may pose significant risk to the subject.
Study Plan
How is the study designed?
Design Details
- Primary Purpose: Prevention
- Allocation: Randomized
- Interventional Model: Crossover Assignment
- Masking: Double
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
Experimental: Broccoli, mustard, vitamin C
770 mg Broccoli Seed Extract (BSE) with 290 mg mustard seed powder (MSP) and 200 mg vitamin C
|
2 capsules per day for 4 weeks
|
Placebo Comparator: Placebo
inactive excipients including microcrystalline cellulose, rice hull, maltodextrin, ascorbyl palmitate and silicon dioxide
|
2 capsules per day for 4 weeks.
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Skin IL-1beta (pg/ml)
Time Frame: Change from pre-study to post-4 weeks supplementation
|
Skin inflammation cytokine
|
Change from pre-study to post-4 weeks supplementation
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Skin elasticity (arbitrary units)
Time Frame: Change from pre-study to post-4 weeks supplementation
|
DermaLab® Combo elasticity probe measurement
|
Change from pre-study to post-4 weeks supplementation
|
Skin pH (arbitrary units)
Time Frame: Change from pre-study to post-4 weeks supplementation
|
DermaLab® Combo pH probe measurement
|
Change from pre-study to post-4 weeks supplementation
|
Skin hydration (arbitrary units)
Time Frame: Change from pre-study to post-4 weeks supplementation
|
DermaLab® Combo hydration probe measurement
|
Change from pre-study to post-4 weeks supplementation
|
Skin pigmentation (melatonin content, no units)
Time Frame: Change from pre-study to post-4 weeks supplementation
|
DermaLab Combo color probe
|
Change from pre-study to post-4 weeks supplementation
|
Transepidermal water loss (TEWL) (g/m2/h)
Time Frame: Change from pre-study to post-4 weeks supplementation
|
DermaLab® Combo TEWL probe measurement
|
Change from pre-study to post-4 weeks supplementation
|
Skin sebum (arbitrary units)
Time Frame: Change from pre-study to post-4 weeks supplementation
|
DermaLab® Combo sebum probe measurement
|
Change from pre-study to post-4 weeks supplementation
|
Collaborators and Investigators
Sponsor
Collaborators
Investigators
- Study Director: Camila A Olson, PhD, Appalachian State University
Study record dates
Study Major Dates
Study Start (Actual)
Primary Completion (Actual)
Study Completion (Actual)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Actual)
Study Record Updates
Last Update Posted (Estimated)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Keywords
Additional Relevant MeSH Terms
Other Study ID Numbers
- HS-22-42
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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