Whole Coffee Fruit Concentrate Pilot Study 1 (WCFC)

Participants (n=23; 20 completing with usable data) will come to UCLA for 1 in-person screening visit and 4 weekly study visits, culminating in a total of 5 study visits. Those who meet eligibility criteria will take part in a baseline test session (before randomization) to collect cognitive and behavioral data as well as baseline plasma BDNF level. There will also be two test sessions at 14 and 28 days of treatment to collect data on the effects of the active and placebo treatments. The questionnaires, cognitive tasks, and diagnostic interviews will be conducted on an outpatient basis at the Semel Institute for Neuroscience and Human Behavior, and the medical procedures will take place at the UCLA Clinical and Translational Research Center (CTRC).

In-person screening procedures:

First, investigators will thoroughly explain the study and obtain informed consent from interested participants. Immediately after consent is obtained, the following questionnaires/ diagnostic interviews will be completed:

• Study Admission & Intake Form- This questionnaire administered at intake collects demographic information (i.e. ethnicity, education, employment), general and mental health history, as well as substance abuse history. This questionnaire takes approximately 15-45 min to complete.
• Psychiatric evaluation using the SCID-IR-TR. The SCID will be administered during intake screening to determine whether the participant meets the DSM-IV-TR criteria for drug dependence and to rule out any major psychiatric disorders (e.g., affective disorders, schizophrenia; Spitzer et al., 1995).
• The International Physical Activity Questionnaires (IPAQ). The purpose of the IPAQ (Craig et al., 2003) is to record any exercise that participants engage in as this may impact findings. It takes approximately 5 min to complete.

Urine testing (40 ml) will be done to test for pregnancy (females only) and drugs of abuse. Urine testing will be done at screening as well as each of the study visits. Once the urine has been analyzed, it will be disposed of in a sanitary manner.

Medical Procedures completed during in-person screening:

Participants will give a blood sample taken for Rapid Plasma Reagin (RPR), and Hepatitis B and C testing [25-ml sample to including a complete chemistry panel (5 ml), hepatic panel (3 ml), Hepatitis B and C, RPR test (7 ml), plasma BDNF (0.1 ml)]. The study physician will inform participants of the implications if their status for RPR, hepatitis B or hepatitis C test results is positive, and will refer participants to further information. Study staff will inform participants with negative RPR, hepatitis B and hepatitis C test results of their health status. Height and weight will be assessed, as body mass index (BMI) values provide inclusion criteria (18 ≤ BMI ≥ 25 kg/m2). In addition, vital signs such as heart-rate (ECG), pulse, and blood pressure will be measured. Plasma BDNF (and other biomarkers) will be measured by a blood sample (finger stick) obtained prior to, and at each weekly visit to UCLA after initiating WCFC or placebo administration.

The maximum time period allowable between the screening visits and attendance at the UCLA CTRC will be 4 weeks. If this time elapses, the potential subject will be screened again.

Study Visit Timeline:

Day Up to -28: Telephone screening Day 0- In-person medical screening, baseline cognitive testing session and rating of subjective mood states. BDNF assay. Randomization to group, and initiation of WCFC/placebo treatment, with 14-day supply provided to the participant.

Day 7- Collection of compliance questionnaire, BDNF assay. Day 14- Cognitive testing and rating of subjective mood states. Collection of compliance questionnaire; re-supply WCFC/placebo; and phlebotomy for blood chemistry and assay of BDNF and other biomarkers.

Day 21- Collection of compliance questionnaire, BDNF assay. Day 28- Completion of WCFC/placebo treatment. Final cognitive testing session and rating of subjective mood states. Collection of compliance questionnaire. Phlebotomy for blood chemistry, and assay of BDNF and other biomarkers.

Study Visit Procedures (~2-3 hrs):

Participants meeting the inclusion criteria will be randomized to receive either WCFC or placebo. During the active treatment time (28 days), they will visit the UCLA Semel Institute on a weekly basis. On day 0 (at baseline) and day 14, WCFC or placebo will be provided, in amounts sufficient for a 2-week supply, so that they can self-administer the respective treatments. At each of these weekly visits, questionnaires regarding compliance will be completed, and blood samples will be taken for assay of BDNF. A cognitive test battery and mood-rating scales will be completed at baseline and at 14 and 28 days of treatment. At the midpoint assessment (14 days) and at completion of treatment (28 days) blood will be drawn for assay of a blood chemistry panel (as at baseline) as well as for biomarkers in addition to BDNF.

Nutraceutical Administration and Dose Justification:

WCFC (NeurofactorTM): A dose of 100 mg will be administered twice daily (once before breakfast and again before dinner). The dose was chosen on the basis of published work from Applied Bioclinical, Inc. (Reyes-Izquierdo et al., 2013; Pietrzkowski et al., in press). Specifically, Reyes-Izquierdo and colleagues (2013) reported that 100 mg of WCFC resulted in increased plasma BDNF by 143% from baseline (n=10, healthy adults aged between 18 and 55 years old). More recently Pietrzkowski and co-workers (unpublished manuscript in press) observed a 90% increase in plasma BDNF during the first 60 minutes after a single 100 mg dose of WCFC. The effect is sustained for 2-3 hours. Therefore, testing will always take place in the morning within 2 hours of WCFC ingestion with breakfast. As a quality control measure, WCFC batches will be tested to delineate the same chemical profile of polyphenols to make sure that if more than one batch is used, the chemical profiles are identical between batches.

Placebo: The placebo will be Nutrim®, supplied by Abclinical and administered twice daily, identical to WCFC treatment (other participant group).

Both WCFC and placebo are in powder form, and will be capsulated in size 1 gelatin capsules, white and blue, distributed in brown plastic bottles (28 caps/bottle: enough for 14 days of treatment). The first bottle will be provided at the time of randomization, and the second bottle will be provided on day 14 for dosing on days 15-28. The bottles will be labeled with the ABC logo and treatment code ABC#7UCLA01 and ABC#7UCLA12.

The following questionnaires that assess mood will be administered during each of the study sessions:

• HAM-D- The Hamilton Depression Rating Scale is a multiple item questionnaire used to provide an indication of depression, and as a guide to evaluate recovery. The questionnaire is designed for adults and is used to rate the severity of their depression by probing mood, feelings of guilt, suicide ideation, insomnia, agitation or retardation, anxiety, weight loss, and somatic symptoms. Assessment time is estimated at 20 minutes. The HAM-D has been used previously, showing a correlation between increased plasma BDNF and improved (decreased) HAM-D scores (e.g., Shimizu et al., 2003, Gonul et al., 2005, Aydemir et al., 2005, Yoshimura et al., 2007).
• PANAS- The Positive and Negative Affect Schedule (Watson et al., 1988) is a 20-item questionnaire that comprises two mood scales, one measuring positive affect and the other measuring negative affect. Each item is rated on a 5-point scale ranging from 1 = very slightly or not at all to 5 = extremely to indicate the extent to which the respondent has felt this way in the indicated time frame. The participant will be instructed to provide these ratings of his or her affective state at the time of assessment.
• Adverse Events Log- At each visit after randomization, any adverse events that occur will be recorded, and evaluated in terms of frequency and severity (mild, moderate, severe) using this form.

The following cognitive tests will be administered during each of the study sessions:

• Hopkins Verbal Learning Test- Revised (HVLT-R): This task is an assessment of verbal memory.
• Continuous Performance Test (CPT): This computer task is an assessment of attention & inhibition.
• Spatial Sternberg: This computer task is an assessment of spatial short term memory.
• Letter Number Sequencing: This task in an assessment of working memory.
• Attention Networks Task: This computer task is an assessment of attention & inhibition.
• Verbal fluency: This task is an assessment of executive function.
• Finger Tapping: This task is an assessment of psychomotor speed.

These tests will assess multiple cognitive domains, and the total battery of tests will take approximately 1.5 hours to complete. Thus, the burden on participants will be low. Participants will be trained on the tasks before the baseline measurement is taken to reduce the effects of learning on performance.

Biochemical Measures:

The biochemical measurements that will be performed during the study will be the determination of plasma BDNF at baseline (conducted during medical screening procedure) and at each weekly visit following the initiation of WCFC and placebo administration, and assay of other biomarkers in serum at the 14- and 28-day test points. Determination of plasma BDNF must be performed at the same time of day for all subjects at all time-points, due to diurnal variation in plasma BDNF levels (Piccini et al., 2008). Weekly BDNF data will be used as an index of compliance with WCFC administration. Data at baseline, 14 days and 28 days will be used in tests of association with behavioral measures.