Glycemic Response of Co-ingesting Carbohydrate and Amino Acids

The study will investigate if consumption of amino acid mixtures results a change in glycemic response of carbohydrate (white rice) and insulinemic response at different doses and timing (before consumption of carbohydrate).

Study Overview

• Status: Completed
• Conditions: Metabolic Diseases
• Intervention / Treatment: Other: Rice and Amino Acid mixtures

Detailed Description

Healthy male subjects will be served with amino acid mixtures in combinations of two doses and three time points along with white rice as control. The combinations were either low or high levels of amino acid mixtures. This was consumed together or 15 min, 30 min before consumption of white rice. Postprandial blood glucose and plasma insulin concentrations were measured at fasting and every 15 min after consumption of treatment meal until 60 min after the consumption of white rice. Subsequent blood sample were taken at 30 min interval until 210min. The glucose and insulin in the blood will be analysed.

• Study Type: Interventional
• Enrollment (Actual): 23
• Phase: Not Applicable

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 21 years to 50 years (ADULT)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• Male
• Chinese ethnicity
• Age between 21-50 years
• Body mass index (BMI) between 18-24.9 kg/m2
• Waist circumference ≤ 90 cm for men
• Normal blood pressure (<120/80 mmHg)
• Fasting blood glucose < 6 mmol/L

Exclusion Criteria:

• dislike the consumption of Essence of Chicken
• participating in competitive sports
• allergic to amino acids and rice
• metabolic diseases (such as diabetes, hypertension and the metabolic syndromes)
• Glucose-6-Phosphate-Dehydrogenase (G6PD) Deficiency
• on prescription medication
• smokers

Study Plan

How is the study designed?

Design Details

• Allocation: RANDOMIZED
• Interventional Model: CROSSOVER
• Masking: NONE

Number of Arms

7

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: White rice control; Subjects will consume white rice as control (50g carbohydrate)	Other: Rice and Amino Acid mixtures; Rice and amino acid mixture
Experimental: co-ingest low dose of amino acid; Subjects will receive 68 ml of amino acid mixture together with white rice	Other: Rice and Amino Acid mixtures; Rice and amino acid mixture
Experimental: co-ingest high dose of amino acid; Subjects will receive 136 ml of amino acid mixture together with white rice	Other: Rice and Amino Acid mixtures; Rice and amino acid mixture
Experimental: pre-load low dose15 min; Subjects will receive 68 ml of amino acid mixture 15 min before consumption of white rice	Other: Rice and Amino Acid mixtures; Rice and amino acid mixture
Experimental: pre-load low dose 30 min; Subjects will receive 68 ml of amino acid mixture 30 min before consumption of white rice	Other: Rice and Amino Acid mixtures; Rice and amino acid mixture
Experimental: pre-load high dose 15 min; Subjects will receive 136 ml of amino acid mixture 15 min before consumption of white rice	Other: Rice and Amino Acid mixtures; Rice and amino acid mixture
Experimental: pre-load high dose 30 min; Subjects will receive 136 ml of amino acid mixture 30 min before consumption of white rice	Other: Rice and Amino Acid mixtures; Rice and amino acid mixture

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Glycemic response	Blood glucose will be assessed via finger prick during fasting and every 15 minutes for the first 60 min after the consumption white rice and subsequently every 30 min interval until 210 min	210 minutes post consumption of carbohydrate
Insulinemic response	Plasma insulin will be assessed via finger prick during fasting and every 15 minutes for the first 60 min after the consumption white rice and subsequently every 30 min interval until 210 min	210 minutes post consumption of carbohydrate

Collaborators and Investigators

• Sponsor: Clinical Nutrition Research Centre, Singapore
• Collaborators: Cerebos Asia Pacific Pte Ltd

Study record dates

Study Major Dates

• Study Start: June 1, 2014
• Primary Completion (Actual): October 1, 2015
• Study Completion (Actual): October 1, 2015

Study Registration Dates

• First Submitted: May 21, 2015
• First Submitted That Met QC Criteria: May 25, 2015
• First Posted (Estimate): May 28, 2015

Study Record Updates

• Last Update Posted (Estimate): January 28, 2016
• Last Update Submitted That Met QC Criteria: January 27, 2016
• Last Verified: January 1, 2016

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Metabolic Diseases
• Other Study ID Numbers: 2014/00366