- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT03929367
PK Sampling After IV Oxytocin and Effects on Sensory Function in Healthy Volunteers
Pharmacokinetics of Intravenous Oxytocin and Effects on Sensory Function in Healthy Volunteers
The primary goal of this protocol is to model change in oxytocin concentrations in plasma after intravenous (IV) administration. Additional measurements will be performed on sensory function that could be influenced by oxytocin during later time periods when blood samples are widely separated by time. This protocol will describe the pharmacokinetics (PK) of intravenous oxytocin in healthy adult men and adult,non-pregnant women. PK samples will be obtained after an injection of intravenous oxytocin 10 IU over 60 seconds.
Blood will be drawn for the PK samples prior to the oxytocin administration and then 11 times after administration.
There are two additional measures which will assess two different aspects of sensory function.
MEASURE 1: Light Touch Frequency Threshold In order to determine the highest frequency that specific nerve fibers can respond to, a simple device that produces an oscillatory / vibratory stimulus will be used on the hand. The subject places fingertips, palm, and palm side of the wrist sequentially and the device is set to slowly decrease the frequency of vibration from 1 kHz until the subject first perceives this. This is repeated three times at each site and takes less than 5 minutes in total. This will be performed before the oxytocin administration and 6 times after the administration of IV Oxytocin.
MEASURE 2: Sustained Heat The surface of the skin of the forearm or leg is heated to 113°F using a computer controlled thermode for a period of 5 minutes. This will be done before the IV Oxytocin and 4 times after the administration. A set of random thermal temperatures (98°F-122°F) will also be applied to the skin of the leg or forearm before the IV Oxytocin administration and 4 times after administration of IV Oxytocin.
Study Overview
Detailed Description
The primary goal of this protocol is to model change in oxytocin concentrations in plasma after intravenous administration, (oxytocin (Pitocin®), 10 IU) the investigators will also acquire tests of sensory function that could be modulated by oxytocin during later time periods when blood samples are widely separated by time. There are two measures which will assess two different aspects of sensory function.
MEASURE 1: Light Touch Frequency Threshold Light touch is subserved by a group of myelinated peripheral nerves with fast conduction in the Aβ range and which are capable of following high frequencies, being responsible for the sensation of vibration. This capability of individual nerve fibers to respond with high frequency also underlies our ability to sense a light touch moving across the body surface as in brushing.
In preclinical studies investigators have shown that, following injury, these light touch fibers lose their ability to follow high frequencies and, in some cases, no longer respond to mechanical stimulation at all. Oxytocin, when perfused around the neuronal cell bodies of these Aβ nerve fibers, partially restores their function, including their response to high frequency stimulation.
In order to determine the highest frequency that Aβ nerve fibers can respond to, the investigators have created a simple device that produces an oscillatory / vibratory stimulus which can be used on the hand. The subject places fingertips, palm, and volar aspect of the wrist sequentially and the device is set to slowly decrease the frequency of vibration from 1 kilohertz (kHz) until the participant first perceives this. This is repeated three times at each site and takes under 5 minutes in total.
The investigators anticipate that oxytocin will have no effect on normal Aβ fibers and that this measure will not change after oxytocin administration. This will provide important data to contrast with the expected efficacy of oxytocin in the setting of injury, to be studied in subsequent protocols.
MEASURE 2: Sustained Heat As part of a protocol used by the investigators in several studies, the surface of the skin of the forearm or leg is heated to 45°C using a computer-controlled thermode for a period of 5 minutes. This typically results in a slowly increasing pain experience, peaking at 5 minutes in the mild to low moderate range and has been tolerated by more than 150 subjects in studies over the past 14 years.
In an interim analysis of an ongoing clinical study in volunteers, the investigators noted that spinal injection of oxytocin, 15 μg (equivalent to 9 IU), that pain from this sustained heat was dramatically reduced over the 5 minutes of stimulus presentation. In contrast, spinal oxytocin had no effect on the stimulus-response relationship of pain to noxious heat over a 42-50°C range when the stimuli were presented for only 5 seconds. These data suggest that oxytocin reduces C-fiber mediated signaling of sustained heat pain, likely through a spinal site of action.
Drugs administered spinally have a much higher concentration in cerebrospinal fluid than plasma. Nonetheless, spinally administered drugs are typically absorbed to an extent and with a time course similar to that after intramuscular injection, and it is conceivable that the effect on sustained heat pain investigators are seeing in the spinal study reflect peripheral effects via systemic absorption. Thus, if the investigators see a similar reduction in response to sustained heat in this protocol of intravenous administration, the investigators will conclude that oxytocin is more likely activating peripherally than spinally.
Study Type
Enrollment (Actual)
Phase
- Phase 4
Contacts and Locations
Study Locations
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North Carolina
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Winston-Salem, North Carolina, United States, 27157
- Wake Forest Baptist Medical Center
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Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Genders Eligible for Study
Description
Inclusion Criteria:
- Male or female > 18 and < 60 years of age, Body Mass Index (BMI) <40.
- Generally in good health as determined by the Principal Investigator based on prior medical history, American Society of Anesthesiologists physical status I or II
- Normal blood pressure (systolic 90-140 mmHg; diastolic 50-90 mmHg) resting heart rate 45-100 beats per minute) without medication
- Female subjects of child-bearing potential and those < 1 year post-menopausal, must be practicing highly effective methods of birth control such as hormonal methods
Exclusion Criteria:
- Hypersensitivity, allergy, or significant reaction to any ingredient of Pitocin®
- Any disease, diagnosis, or condition (medical or surgical) that, in the opinion of the Principal Investigator, would place the subject at increased risk (active gynecologic disease in which increased tone would be detrimental e.g., uterine fibroids with ongoing bleeding), compromise the subject's compliance with study procedures, or compromise the quality of the data
- Women who are pregnant (positive result for serum pregnancy test at screening visit), women who are currently nursing or lactating, women that have been pregnant within 2 years
- Subjects with neuropathy, chronic pain, diabetes mellitus, or taking benzodiazepines or pain medications on a daily basis.
Study Plan
How is the study designed?
Design Details
- Primary Purpose: BASIC_SCIENCE
- Allocation: NA
- Interventional Model: SINGLE_GROUP
- Masking: NONE
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
OTHER: Oxytocin (Pitocin®), 10 IU
Oxytocin 10 IU administered once per intravenous injection
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Single IV administration of oxytocin
Other Names:
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Oxytocin Concentration
Time Frame: 2 minutes
|
Plasma concentrations of oxytocin at defined times after intravenous infusion
|
2 minutes
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Oxytocin Concentration
Time Frame: 5 minutes
|
Plasma concentrations of oxytocin at defined times after intravenous infusion
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5 minutes
|
Oxytocin Concentration
Time Frame: 10 minutes
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Plasma concentrations of oxytocin at defined times after intravenous infusion
|
10 minutes
|
Oxytocin Concentration
Time Frame: 15 minutes
|
Plasma concentrations of oxytocin at defined times after intravenous infusion
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15 minutes
|
Oxytocin Concentration
Time Frame: 30 minutes
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Plasma concentrations of oxytocin at defined times after intravenous infusion
|
30 minutes
|
Oxytocin Concentration
Time Frame: 45 minutes
|
Plasma concentrations of oxytocin at defined times after intravenous infusion
|
45 minutes
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Oxytocin Concentration
Time Frame: 60 minutes
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Plasma concentrations of oxytocin at defined times after intravenous infusion
|
60 minutes
|
Oxytocin Concentration
Time Frame: 90 minutes
|
Plasma concentrations of oxytocin at defined times after intravenous infusion
|
90 minutes
|
Oxytocin Concentration
Time Frame: 120 minutes
|
Plasma concentrations of oxytocin at defined times after intravenous infusion
|
120 minutes
|
Oxytocin Concentration
Time Frame: 180 minutes
|
Plasma concentrations of oxytocin at defined times after intravenous infusion
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180 minutes
|
Oxytocin Concentration
Time Frame: 240 minutes
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Plasma concentrations of oxytocin at defined times after intravenous infusion
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240 minutes
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Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Light Touch Frequency Threshold
Time Frame: 5 minutes
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The frequency at which the vibration is perceived after intravenous oxytocin administration
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5 minutes
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Light Touch Frequency Threshold
Time Frame: 20 minutes
|
The frequency at which the vibration is perceived after intravenous oxytocin administration
|
20 minutes
|
Light Touch Frequency Threshold
Time Frame: 45 minutes
|
The frequency at which the vibration is perceived after intravenous oxytocin administration
|
45 minutes
|
Light Touch Frequency Threshold
Time Frame: 60 minutes
|
The frequency at which the vibration is perceived after intravenous oxytocin administration
|
60 minutes
|
Light Touch Frequency Threshold
Time Frame: 120 minutes
|
The frequency at which the vibration is perceived after intravenous oxytocin administration
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120 minutes
|
Light Touch Frequency Threshold
Time Frame: 180 minutes
|
The frequency at which the vibration is perceived after intravenous oxytocin administration
|
180 minutes
|
Sustained Heat
Time Frame: 31 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
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31 minutes
|
Sustained Heat
Time Frame: 32 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
|
32 minutes
|
Sustained Heat
Time Frame: 33 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
|
33 minutes
|
Sustained Heat
Time Frame: 34 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
|
34 minutes
|
Sustained Heat
Time Frame: 35 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
|
35 minutes
|
Sustained Heat
Time Frame: 61 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
|
61 minutes
|
Sustained Heat
Time Frame: 62 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
|
62 minutes
|
Sustained Heat
Time Frame: 63 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
|
63 minutes
|
Sustained Heat
Time Frame: 64 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
|
64 minutes
|
Sustained Heat
Time Frame: 65 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
|
65 minutes
|
Sustained Heat
Time Frame: 121 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
|
121 minutes
|
Sustained Heat
Time Frame: 122 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
|
122 minutes
|
Sustained Heat
Time Frame: 123 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
|
123 minutes
|
Sustained Heat
Time Frame: 124 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
|
124 minutes
|
Sustained Heat
Time Frame: 125 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
|
125 minutes
|
Sustained Heat
Time Frame: 181 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
|
181 minutes
|
Sustained Heat
Time Frame: 182 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
|
182 minutes
|
Sustained Heat
Time Frame: 183 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
|
183 minutes
|
Sustained Heat
Time Frame: 184 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
|
184 minutes
|
Sustained Heat
Time Frame: 185 minutes
|
0-10 verbal pain score to a 45 degree Celsius stimulus after intravenous oxytocin administration; higher score denotes worse outcome.
|
185 minutes
|
Collaborators and Investigators
Investigators
- Principal Investigator: James Eisenach, MD, Wake Forest University Health Sciences
Study record dates
Study Major Dates
Study Start (ACTUAL)
Primary Completion (ACTUAL)
Study Completion (ACTUAL)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (ACTUAL)
Study Record Updates
Last Update Posted (ACTUAL)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Additional Relevant MeSH Terms
Other Study ID Numbers
- IRB00056413
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
product manufactured in and exported from the U.S.
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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