- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT04359082
Cocoa-rich Bioflavanol Supplementation Effects During Cold Exposure
April 27, 2021 updated by: United States Army Research Institute of Environmental Medicine
The Effects of Cocoa-rich Bioflavanol Supplementation on Manual Dexterity During Cold Exposure
The primary purpose is to test whether cocoa-rich bioflavanols can improve blood flow to the hand and fingers and improve hand function/dexterity during cold exposure.
Secondary purpose is to understand whether bioflavanol supplementation can change the gut microbiome.
Study Overview
Status
Recruiting
Conditions
Intervention / Treatment
Detailed Description
Loss of manual dexterity significantly impacts Warfighter effectiveness and lethality on the cold-weather battlefield.
Identifying countermeasures that improve hand and finger temperatures, hand and finger blood flow, manual dexterity, and thermal comfort may lead to better Warfighter performance.
A primary purpose of this study is to test the efficacy of a commercial off-the-shelf cocoa-rich bioflavanol product on hand/finger blood flow and temperature, manual dexterity, and thermal comfort during whole-body cold exposure.
Another primary purpose is to determine the effect of bioflavanols on the finger cold-induced vasodilation (CIVD) response to cold-water immersion.
Secondary purposes are to determine if: a) cocoa-rich flavanols stimulate the growth of beneficial gut bacteria and b) impact attention and effort perception during dexterity task completion in the cold.
Two experimental studies (n = 20 in each study, 10 women and 10 men, 18-49 yr) will be conducted.
Experiment #1 consists of immersing the middle finger in cold water (4°C) for 30 minutes; experiment #2 consists of whole-body cold exposure (8°C air, 90-min exposure, wearing cold-weather clothing but bare-handed).
In both experiments, outcomes will be measured after both acute flavanol or placebo ingestion and chronic ingestion of flavanol/placebo (8 days of supplementation).
Daily flavanol ingestion will be 900 mg/day.
Volunteers will serve as their own controls in this crossover, double-blind research design.
The washout period between flavanol and placebo treatments will be at least 2 weeks (longer for women who will be tested only during the follicular phase).
Measurements during these tests include skin temperature, skin blood flow, fine and gross motor dexterity, thermal comfort, and effort perception.
Fecal samples, in both experiments, will be collected before and after 8 days of cocoa-rich bioflavanol supplementation to examine if bioflavanol supplementation increases the number of beneficial bacteria in the gut microbiome.
Information from this study will inform combat nutrition developers about the efficacy of cocoa-rich bioflavanols and possible inclusion into cold-weather field rations.
Study Type
Interventional
Enrollment (Anticipated)
40
Phase
- Not Applicable
Contacts and Locations
This section provides the contact details for those conducting the study, and information on where this study is being conducted.
Study Contact
- Name: John W Castellani, PhD
- Phone Number: 508-206-2195
- Email: john.w.castellani.civ@mail.mil
Study Contact Backup
- Name: Billie Alba, PhD
- Phone Number: 508-206-2171
- Email: billie.k.alba.civ@mail.mil
Study Locations
-
-
Massachusetts
-
Natick, Massachusetts, United States, 01760
- Recruiting
- John W Castellani
-
Contact:
- John W Castellani, PhD
- Phone Number: 508-206-2195
- Email: john.w.castellani.civ@mail.mil
-
Principal Investigator:
- John W Castellani, PhD
-
Principal Investigator:
- Billie K Alba, PhD
-
-
Participation Criteria
Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.
Eligibility Criteria
Ages Eligible for Study
18 years to 49 years (Adult)
Accepts Healthy Volunteers
No
Genders Eligible for Study
All
Description
Inclusion Criteria:
- Age - 18-49 years (17-49 for active military).
- Refrain from the following for 2 weeks before and during the study (including the washout period): consumption of flavanol rich foods including cocoa, chocolate, apples, red, white, and sweet wine, apricots, blueberries, peaches/nectarines, plums, grapes, strawberries, pecans (no more than ½ cup/day, pistachios, (no more than ½ cup per day), and apple juice.
- Participants not to change their daily intakes of tea (green or black) or coffee.
Exclusion Criteria:
- History of cold injuries.
- Raynaud's syndrome.
- Cold-induced asthma/bronchospasm
- Difficulty swallowing pills.
- Previous hand/finger injuries that impair dexterity and hand function.
- Metal hardware (plates/screws) in the forearms and hands.
- Blood donation in last 8 weeks.
- Medicine use (including any over the counter medication such as Tylenol, Advil, Sudafed, etc…), with the exception of birth control and a multi-vitamin.
- Known allergies to medical adhesives or cocoa/chocolate.
- History of disease of the gastrointestinal tract including (but not limited to) diverticulosis, diverticulitis and inflammatory bowel disease, peptic ulcer disease, Crohn's disease, ulcerative colitis; or previous gastrointestinal surgery.
- No planned MRI during the study or within 2 days after completing a cold test.
- Use of dietary supplements (to include probiotics and prebiotics), with exception of multi-vitamin containing up to 100% of the recommended daily allowance (RDA).
- No exercise or smoking within 8 hours of testing.
- Pregnant or breastfeeding.
- Oral antibiotic use within 3 months of study participation.
- Inability or unwillingness to not consume fermented food products or prebiotic-containing food products for 2 weeks prior to and throughout study participation. Examples include kefir, kombucha, aged cheese, pickles, sauerkraut, sour cream, tempeh, yogurt, kimchi, miso, vinegar, sourdough bread, wine and beer.
- Colonoscopy within 3 months of study participation.
- On average has a bowel movement less frequently than every other day.
Study Plan
This section provides details of the study plan, including how the study is designed and what the study is measuring.
How is the study designed?
Design Details
- Primary Purpose: Basic Science
- Allocation: Randomized
- Interventional Model: Crossover Assignment
- Masking: Double
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
Placebo Comparator: Placebo
Placebo for 8 straight days
|
Placebo pill has no bioflavanols with 30 mg theobromine and 10 mg caffeine
|
Experimental: Bioflavanol
Bioflavanol supplementation for 8 days at 900 mg flavanol per day
|
CocoaVia is a dietary supplement that has concentrated bioflavanols of 225 mg flavanols per pill along with 30 mg theobromine and 10 mg caffeine
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Cold induced vasodilation response
Time Frame: 30 minutes
|
Skin/finger temperature changes during finger immersion in 4°C water
|
30 minutes
|
Manual dexterity-PP
Time Frame: 2 hours
|
Purdue Pegboard score during 8°C air exposure.
Score is the number of pegs placed into board.
|
2 hours
|
Manual dexterity-MRM
Time Frame: 2 hours
|
Minnesota Rate of Manipulation dexterity score during 8°C air exposure.
Score is the number of pieces put together during times period.
|
2 hours
|
Hand and finger temperatures
Time Frame: 2 hours
|
Hand and finger temperatures during cold air exposure in 8
|
2 hours
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Gut microbiome
Time Frame: 8 days
|
How do targeted populations (Bifidobacterium spp., Lactobacillus spp., C. coccoides-Eubacterium rectale, Faecalibacterium prausnitzii, Prevotella/Bacteroides, Roseburia, and Akkermansia muciniphila) change as a result of bioflavanol supplementation
|
8 days
|
Collaborators and Investigators
This is where you will find people and organizations involved with this study.
Investigators
- Principal Investigator: John W Castellani, PhD, US Army Research Institute of Environmental Medicine
Study record dates
These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.
Study Major Dates
Study Start (Actual)
January 13, 2020
Primary Completion (Anticipated)
May 1, 2021
Study Completion (Anticipated)
September 1, 2021
Study Registration Dates
First Submitted
March 9, 2020
First Submitted That Met QC Criteria
April 21, 2020
First Posted (Actual)
April 24, 2020
Study Record Updates
Last Update Posted (Actual)
April 30, 2021
Last Update Submitted That Met QC Criteria
April 27, 2021
Last Verified
April 1, 2021
More Information
Terms related to this study
Other Study ID Numbers
- 18-27-H
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
No
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
No
Studies a U.S. FDA-regulated device product
No
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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