BOXR1030 T Cells in Subjects With Advanced GPC3-Positive Solid Tumors (DUET-1)

A First-in-Human, Phase 1/2, Dose Escalation Study of BOXR1030 T Cells in Subjects With Advanced GPC3-Positive Solid Tumors

This is a first-in-human (FIH), Phase 1/2, open-label, multicenter study to assess safety and determine the recommended Phase 2 dose (RP2D) of BOXR1030 administration after lymphodepleting chemotherapy (LD chemotherapy) in subjects with glypican-3 positive (GPC3+) advanced solid tumors.

Study Overview

• Status: Recruiting
• Conditions: Hepatocellular Carcinoma; Squamous Cell Carcinoma of the Lung; Merkel Cell Carcinoma; Myxoid/Round Cell Liposarcoma
• Intervention / Treatment: Biological: CAR-GPC3 T Cells

Detailed Description

This is a FIH, Phase 1/2, open-label, multicenter study to assess safety and determine the maximum tolerated dose (MTD) and/or RP2D of BOXR1030 administration after LD chemotherapy in subjects with GPC3+ advanced solid tumors. After signing informed consent and completing all screening assessments, eligible subjects will be enrolled and undergo leukapheresis to obtain T cells for BOXR1030 manufacturing. Subjects will receive a 3-day LD chemotherapy regimen with fludarabine and cyclophosphamide, administered according to institutional standard practice for these drugs, including inpatient administration as appropriate. Subjects must be hospitalized for BOXR1030 administration and will remain hospitalized for 10 days after the infusion. For 28 days after BOXR1030 administration, all subjects must stay within a distance that requires no more than 2 hours of travel to the study site.

During the Post-treatment Evaluation Period (within 6 months after BOXR1030 administration), study visits will occur daily for the first week, twice in the second week, and then once weekly at Weeks 3, 4, 6, 9, 12, 15, 18, and 24. Safety (targeted physical examination, adverse event [AE] assessment, and clinical laboratory tests) will be evaluated and samples will be collected for endpoint analyses. For 28 days after BOXR1030 administration, subjects will be required to monitor their temperature and complete neurological evaluation via the immune effector cell-associated encephalopathy assessment tool every day (to be administered by site staff during clinical visits and by a caregiver at home on non-clinic days). At regular intervals, antitumor activity will be assessed per RECIST 1.1 and iRECIST criteria.

After 6 months of follow-up from BOXR1030 administration, subjects will enter the Long-term Follow-up Period for a total duration of 15 years after BOXR1030 dosing. Study visits are scheduled every 3 months from Month 9 to Month 24, every 6 months thereafter until Year 5, and then annually through Year 15. Long-term follow-up assessments will focus on long-term safety and disease status. Subjects whose disease does not progress before Week 24 will enter the Long-term Follow-up at Month 7. Survival status will be checked at these visits.

• Study Type: Interventional
• Enrollment (Estimated): 110
• Phase: Phase 2; Phase 1

Contacts and Locations

• Study Contact: Name: Richard Kapsa; Phone Number: (+420) 2241 74448; Email: kapsa@sotio.com

Study Locations

• United States
  • Texas
    • Dallas, Texas, United States, 75246
      • Recruiting
      • Baylor Scott and White Research Institute
      • Contact: Joyce Ghormley; Phone Number: 214-818-8961; Email: Joyce.Ghormley@BSWHealth.org
      • Principal Investigator: Scott Paulson, MD
    • Houston, Texas, United States, 77030-4008
      • Recruiting
      • University of Texas MD Anderson Cancer Center
      • Principal Investigator: Ecaterina Dumbrava, MD
      • Contact: Amber Kennon; Phone Number: 713-745-8661; Email: amkennon@mdanderson.org
  • Wisconsin
    • Milwaukee, Wisconsin, United States, 53226
      • Recruiting
      • Froedtert and Medical College of Wisconsin
      • Contact: Julia Frasch; Phone Number: 414-805-5026; Email: jfrasch@mcw.edu
      • Contact: Kayla Peterson; Phone Number: 414-805-0769; Email: Kpeterson@mcw.edu
      • Principal Investigator: Jonathan Thompson, MD

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 80 years (Adult, Older Adult)
• Accepts Healthy Volunteers: No

Description

Inclusion Criteria:

1. Aged 18 to 80 years at time of enrollment
2. Body weight >/= 50kg
3. Able to provide a recent tumor specimen taken within 6 months prior to signing consent and after the initiation of the subject's most recent systemic anti-cancer therapy, for GPC3 expression assessment by immunohistochemistry (IHC). Previously collected tumor tissue older than 6 months at time of GPC3 IHC testing or collected prior to initiation of current or last systemic therapy may be permitted for GPC3 prescreening. If prescreening sample is found to be GPC3+, a new tumor biopsy will be needed to confirm tumor remains GPC3+ in order to proceed.
4. Histologically confirmed advanced unresectable or metastatic hepatocellular carcinoma (HCC), squamous cell carcinoma (SCC) of the lung, myxoid/round cell liposarcoma (MRCLS), or Merkel cell carcinoma (MCC) with GPC3 overexpression by IHC. Subjects must consent to IHC testing in a separate informed consent. Note: Tumor samples will be sent to a central laboratory for GPC3 expression analysis.
5. Documentation of disease progression or refractory disease or intolerance to prior lines of standard-of-care therapies. Patients with tumors with genetic alterations and mutations (e.g., breast cancer gene, epidermal growth factor receptor mutations, and anaplastic lymphoma kinase translocation) who have approved targeted therapies available for their cancer will need to have been treated with such approved therapies or refused such approved targeted therapy for their cancer prior to enrolling in this study.
6. Life expectancy >16 weeks
7. Have adequate organ function (renal/hepatic/pulmonary)
8. Left ventricular ejection fraction ≥50% by multiple-gated acquisition scan or echocardiogram
9. Eastern Cooperative Group performance status of 0 to 1

10. For subjects with HCC:

    • Child-Pugh Score of A
    • No fibrolamellar carcinoma or mixed hepatocellular cholangiocarcinoma histology
    • No grade 2 or grade 3 ascites based on the European Association for the Study of Liver guidelines.
11. A minimum of 2 sites of disease, including at least 1 site that is measurable by RECIST 1.1 criteria to ensure sufficient disease for response assessment. At least 1 of the other lesions must be considered adequate for Protocol-required tumor biopsy.

12. Adequate wash-out of prior systemic therapy for underlying malignancy, relative to leukapheresis:

    • Last dose of any antineoplastic treatment must be at least 2 weeks before leukapheresis.
    • Last dose of any investigational agent must be at least 3 half-lives of the treatment, or 28 days, before leukapheresis (whichever is shorter).

Adequate wash-out of prior systemic therapy for underlying malignancy, relative to LD chemotherapy:

Subjects may receive an additional dose of their last received form of therapy at the same dose and dosing schedule following leukapheresis as bridging therapy; however, the required wash-out period prior to start of LD chemotherapy must be adhered to:

• The last dose of systemic nitrosourea or systemic mitomycin-C must be at least 6 weeks before the first dose of LD chemotherapy in this study.
• For all other systemic cytotoxic chemotherapy, the wash-out must be the duration of a full cycle of that therapy. For example, if the prior therapy is given in 3-week cycles, there must be at least 3 weeks between the last dose of that therapy and the first dose of LD chemotherapy in this study. If the prior therapy is administered more frequently than every 2 weeks, a minimum 2-week wash-out is required.
• For biologic therapy (e.g., antibodies) the wash-out must be either the duration of the biologic agent dosing interval, or 3 weeks, whichever is shorter.
• For small molecule therapies, the wash-out must be 5 half-lives of the drug.
• For any investigational agent, the wash-out must be 3 half-lives of the investigational agent, or 4 weeks, whichever is shorter.

Note: Local radiation of lesions is allowed if indicated for palliation requiring 1 week wash-out prior to start of BOXR1030 dosing if < 2 weeks of radiotherapy for non-central nervous system (CNS) disease; locally treated lesions will be considered non-target lesions. Hormone ablation is also allowed as clinically indicated.

Exclusion Criteria:

1. Prior treatment with adoptive cell therapy (e.g., CAR T-cell therapy, natural killer cell therapy, engineered T-cell receptor therapy).
2. History of allogenic hematopoietic stem cell transplant.
3. Known untreated CNS tumors or brain metastasis. Subjects are eligible if CNS metastases are asymptomatic, have been treated with radiotherapy for at least 1 month prior to informed consent, are off corticosteroids and have neurologically returned to baseline (residual signs or symptoms related to the CNS treatment are permitted). Imaging obtained for the purpose of CNS metastases management performed during screening must document radiographic stability of CNS lesions for at least 1 month prior to leukapheresis and be performed after completion of any CNS directed therapy. If brain scans are performed, magnetic resonance scans are preferred; however, computed tomography scans are acceptable if magnetic resonance imaging is medically contraindicated. CNS evaluation for subjects with no suspicion of brain tumors in their history is not required for the study. Subjects with known leptomeningeal metastases are excluded.
4. Subjects who have not recovered to < 1 or baseline from all AEs due to previous therapies (subjects with ≤ grade 2 peripheral neuropathy that has been stable for at least 4 weeks or < grade 2 endocrine-related AEs that has been stable for at least 4 weeks on replacement therapy).
5. Planned use of any antineoplastic treatment or investigational agent from the time of the first dose of LD chemotherapy through the end of study participation, except for allowed local radiation of lesions for palliation (to be considered non-target lesions after treatment) and hormone ablation.

6. Uncontrolled or life-threatening symptomatic concomitant disease including clinically significant gastrointestinal bleeding or pulmonary hemorrhage within 4 weeks before screening, known symptomatic human immunodeficiency virus (HIV) positive with an acquired immunodeficiency syndrome-defining opportunistic infection within the past 12 months prior to screening, or a current CD4 count <350 cells/µL, symptomatic active hepatitis B or C checked at screening, or active tuberculosis therapy.

   Subjects with HIV are eligible if:

   • They have received antiretroviral therapy (ART) as clinically indicated for at least 12 months prior to starting LD therapy and have an HIV viral load less than 40 copies/mL prior to start of LD therapy.
   • They continue on ART as clinically indicated while enrolled on study.
   • CD4 counts> 350 cells/µL and CD4 counts and viral load are monitored per standard of care by a local health care provider.
   • They are fully vaccinated against SARS-CoV-2.
7. Has received prior radiotherapy within 2 weeks of the start of BOXR1030. Subjects must have recovered from all radiation-related toxicities, not require corticosteroids, and not have had severe radiation pneumonitis.
8. Potentially life-threatening second malignancy requiring systemic treatment within the last 3 years (i.e., subjects with a history of prior malignancy are eligible if treatment was completed at least 3 years before entering the Treatment Period and the subject has no evidence of disease) or which would impede evaluation of treatment response.
9. Clinically significant (i.e., active) cardiovascular disease: cerebral vascular accident/stroke (<6 months prior to enrollment), myocardial infarction (<6 months prior to enrollment), unstable angina, congestive heart failure (New York Heart Association Classification Class II), or the presence of any condition that can increase proarrhythmic risk (e.g., hypokalemia, bradycardia, heart block) including any new, unstable, or serious cardiac arrhythmia requiring medication, or other baseline arrhythmia that might interfere with interpretation of electrocardiograms on study (e.g., bundle branch block).
10. Has an active infection excluding controlled HIV.
11. Has an active autoimmune disease requiring systemic (immunosuppressive) therapy.

NOTE: Additional inclusion/exclusion criteria may apply. The above information is not intended to be an exhaustive list of considerations for potential participation in a clinical trial.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: N/A
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

1

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: GPC3+ solid tumors; One time intravenous administration of BOXR1030 after completion of cyclophosphamide and fludarabine LD chemotherapy	Biological: CAR-GPC3 T Cells; Five dose levels each with LD chemotherapy; Other Names: BOXR1030

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Dose limiting toxicity	Defined using National Cancer Institute Common Terminology Criteria for Adverse Events (NCI CTCAE) version 5.0 or American Society for Transplantation and Cellular Therapy (ASTCT) criteria for cytokine release syndrome or immune effector cell-associated neurotoxicity syndrome.	From the time of BOXR1030 administration (Study Day 1) through 28 days after BOXR1030 administration (Study Day 28/Week 4)
MTD	Defined as the dose that maximizes the probability of targeted toxicity among doses that satisfy the escalation with overdose control criterion.	From the time of BOXR1030 administration (Study Day 1) through 28 days after BOXR1030 administration (Study Day 28/Week 4)
RP2D	The RP2D may be the same as the MTD, a previously tested dose, or an intermediate/alternative dose below the MTD that is yet unexplored. Alternatively, the RP2D may be selected on the basis of observed safety and activity in dose escalation before the MTD is reached.	From the time of BOXR1030 administration (Study Day 1) through 28 days after BOXR1030 administration (Study Day 28/Week 4)
Treatment-emergent AEs (TEAEs)	Type, frequency, and severity of TEAEs; clinically significant abnormal safety laboratory findings; and vital signs. TEAEs and laboratory findings according to NCI CTCAE version 5.0 and ASTCT criteria.	From the time of BOXR1030 administration (Study Day 1) through Week 24

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Overall response rate	Defined according to RECIST 1.1 criteria.	From the time of BOXR1030 administration (Study Day 1) until disease progression/recurrence or start of new anti-cancer therapy, whichever came first, assessed up to approximately 15 years
Best overall response	Defined as the best response recorded from the date of treatment to disease progression or death.	From the time of BOXR1030 administration (Study Day 1) until disease progression or death, whichever came first, assessed up to approximately 15 years
Duration of response	Defined as the time from the first achieved response (complete or partial) to the first date of radiological progression.	From the date of response for patients with response (complete or partial) until disease progression or death, whichever came first, assessed up to approximately 15 years
Progression-free survival	Defined as the time from the date of treatment to the first date of radiological progression or death.	From the time of BOXR1030 administration (Study Day 1) until disease progression or death, whichever came first, assessed up to approximately 15 years
Clinical benefit rate	Defined as the total number (or percentage) of patients who achieved a complete response, partial response, or had stable disease for 6 months or longer.	From the time of BOXR1030 administration (Study Day 1) until disease progression or death, whichever came first, assessed up to approximately 15 years
Time to response	Defined as the time from the date of treatment to the first objective tumor response.	From the time of BOXR1030 administration (Study Day 1) until response, assessed up to approximately 15 years
Time to progression	Defined as the time from the date of treatment to disease progression.	From the time of BOXR1030 administration (Study Day 1) until disease progression, assessed up to approximately 15 years
BOXR1030 T-cell levels in blood	BOXR1030 T-cell levels in blood will be analyzed.	From the time of BOXR1030 administration (Study Day 1) until the end of the study, assessed up to approximately 15 years
BOXR1030 T-cell characterization in blood	DNA samples will be analyzed to detect BOXR1030 transgene levels (GPC3 chimeric antigen receptor [CAR]). Peripheral blood mononuclear cells will be used to evaluate immune cell surface markers along with GPC3 CAR for characterization of BOXR1030 T cells in blood.	From the time of BOXR1030 administration (Study Day 1) until the end of the study, assessed up to approximately 15 years
Levels of inflammatory markers	Inflammatory markers will include, e.g., ferritin, C-reactive protein, erythrocyte sedimentation rate, and triglycerides.	From the time of BOXR1030 administration (Study Day 1) until the end of the study, assessed up to approximately 15 years

Collaborators and Investigators

• Sponsor: Sotio Biotech Inc.
• Collaborators: SOTIO Biotech AG; SOTIO Biotech a.s.
• Investigators: Study Director: Richard Sachse, M.D., Ph.D., SOTIO Biotech AG

Publications and helpful links

General Publications

• Hickman TL, Choi E, Whiteman KR, Muralidharan S, Pai T, Johnson T, Parikh A, Friedman T, Gilbert M, Shen B, Barron L, McGinness KE, Ettenberg SA, Motz GT, Weiss GJ, Jensen-Smith A. BOXR1030, an anti-GPC3 CAR with exogenous GOT2 expression, shows enhanced T cell metabolism and improved anti-cell line derived tumor xenograft activity. PLoS One. 2022 May 4;17(5):e0266980. doi: 10.1371/journal.pone.0266980. eCollection 2022.

Study record dates

Study Major Dates

• Study Start (Actual): May 18, 2022
• Primary Completion (Estimated): April 1, 2026
• Study Completion (Estimated): December 1, 2041

Study Registration Dates

• First Submitted: November 2, 2021
• First Submitted That Met QC Criteria: November 2, 2021
• First Posted (Actual): November 15, 2021

Study Record Updates

• Last Update Posted (Estimated): January 11, 2024
• Last Update Submitted That Met QC Criteria: January 8, 2024
• Last Verified: January 1, 2024

More Information

Terms related to this study

• Keywords: HCC, SCC, MCC, MRCLS
• Additional Relevant MeSH Terms: Virus Diseases; Infections; Respiratory Tract Diseases; Neoplasms, Connective and Soft Tissue; Neoplasms by Histologic Type; Neoplasms; Lung Diseases; Neoplasms by Site; Adenocarcinoma; Neoplasms, Glandular and Epithelial; Respiratory Tract Neoplasms; Thoracic Neoplasms; Neuroectodermal Tumors; Neoplasms, Germ Cell and Embryonal; Neoplasms, Nerve Tissue; DNA Virus Infections; Tumor Virus Infections; Neuroendocrine Tumors; Sarcoma; Neoplasms, Adipose Tissue; Polyomavirus Infections; Carcinoma, Neuroendocrine; Lung Neoplasms; Carcinoma; Carcinoma, Merkel Cell; Liposarcoma; Liposarcoma, Myxoid
• Other Study ID Numbers: B1030-101

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: Yes
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No