Treatment Outcome in Major Depressive Disorder

Predicting Treatment Outcome in Major Depressive Disorder

Major Depressive Disorder (MDD) is one of the most severe and frequently occurring brain disorders worldwide. It has been linked to serotonergic dysfunction, sexual dysfunction, vulnerability to stress and neuro-inflammation. However, at the same time the etiological understanding is limited. Most antidepressants act on the serotonin (5- HT) system, yet between 30-50 % of patients with MDD does not respond successfully to 5-HT acting drugs. Recent experimental models from our group suggest that cerebral 5-HT levels in vivo can be indexed through molecular brain imaging of the 5-HT 4 receptor (5-HT4R) with a novel Positron Emission Tomography (PET) ligand (11C-SB207145). Also, our human studies have confirmed that cerebral synaptic 5-HT is inversely related to 5-HT4R binding and this technique thus can be used to investigate the role of 5-HT tone in the brain in MDD with differential responses to standard antidepressant treatment. By using multimodal neuroimaging technology, we aim to determine the status of the 5-HT system prior to and after either successful or failed neuropharmacological intervention in a non-randomized longitudinal open clinical trial. 100 untreated patients with moderate to severe MDD will be included. Data collection from various neurobiological domains (i.e, 5-HT4R PET imaging, Magnetic Resonance Imaging (MRI), functional MRI (fMRI), electroencephalogram (EEG), psychometrics, neuropsychological tests, and peripheral biomarkers) will be conducted before, during and after 12 weeks of antidepressant treatment. The objective is to identify predictors of pharmacological antidepressant treatment response in depressed individuals before and after 8 weeks of antidepressant treatment.

Study Overview

• Status: Completed
• Conditions: Major Depressive Disorder
• Intervention / Treatment: Drug: Escitalopram; Drug: Duloxetine

Detailed Description

Study population and study program:

Patients will be recruited through a unique new central referral site for "depression packages" in The Mental Health Services in the Capital Region of Denmark. 100 patients with MDD, 18-65 years of age, with moderate to severe single or recurrent episode of MDD (Hamilton 17 item (HAMD-17) score > 17) will be recruited through this portal. All diagnoses will be confirmed by a specialist in psychiatry.

Before initiation of pharmacological antidepressant treatment with escitalopram, patients will receive baseline examinations as follows: 1) 5-HT4R imaging with 11C-SB207145 PET-scan, 2) EEG examinations, 3) structural MRI, 4) functional MRI , 5) neuropsychological testing, 6) peripheral markers of immune-active cell responses, oxidative stress, cortisol-levels, RNA, genotypes and epigenetic factors will be measured in urine, saliva and peripheral blood at baseline and across the study period. Repeated measures across the study period include: 7) psychometrics by using self-reported questionnaires covering trait and state, including mental distress related to depression and other psychopathology, 8) neuropsychological examinations as well as 9) clinical follow-up with interview based ratings of mental status.

Patients will be treated with escitalopram at flexible doses of 10-20 mg/day adjusted depending on effects and side effects, and participate in clinical follow-up sessions at week 1, 2, 4 and 8. Patients with no response to escitalopram after 4 weeks will be shifted to a secondary pharmacological treatment (duloxetine). A final visit to determine longer-term clinical outcome will be performed at week 12. Compliance, side-effects to antidepressant treatment, and depressive symptoms will be monitored at each follow-up session.

The Hamilton 6 items (HAMD-6) subscale has recently shown to be more sensitive to antidepressant response (Østergaard et al), and will be used to identify treatment response in patients. Patients with > 50 % reduction in HAMD-6 after 4 weeks will be defined as early responders, and those with additional < 5 points on the HAMD-6 scale after 8 weeks will be considered in remission. Patients with >25% response at week 4 and < 50% reduction in HAMD-6 after full intervention will be considered non-responders. The assessment program including brain imaging with PET 11C-SB207145 will be conducted before drug intervention is initiated and, depending on treatment outcome, again after 8 weeks of antidepressant treatment in 20 patients in remission (remitters) and 20 non-responding patients (non-responders).

UPDATE: As per April 3, 2017 the rescanned group has been expanded to include various response patterns in order to capture rescan data from patients on a spectrum from poor to excellent treatment response, since out of the first 17 included patients only 1 patient fulfilled the non-responder criterion defined above. Accordingly, the clinical outcome parameter in the context will be changes in HAM-D6 from baseline at week 8.

Healthy controls:

From previous studies conducted at the Neurobiological Research Unit, there is access to brain imaging data and baseline PET-SB207145 brain images among healthy controls as well as an associated biobank with stored blood specimens. During the trial, additional healthy controls will be included and will receive baseline examinations and repeated neuropsychological testing after 12 weeks.

Hypotheses:

1. 5-HT4R binding (as imaged by 11C-SB207145 PET) before treatment will predict antidepressant treatment outcome (remission vs non-response) in depressed patients; higher binding at baseline is hypothesized to be associated with a better treatment outcome. Likewise, secondary, 5-HT4R binding (as imaged by 11C-SB207145 PET) before treatment is expected to be associated with the magnitude of change from baseline in Hamilton score (HAMD-6) at 8 weeks.
2. Remitters will display a greater reduction in 5-HT4R binding (as imaged by 11C-SB207145 PET) after antidepressant treatment relative to non-responders.
3. Patients with MDD will have a higher 5-HT4R binding (as imaged by 11C-SB207145 PET) relative to healthy controls.
4. High amygdala reactivity in an "emotional faces" fMRI paradigm before treatment predicts remission in response to antidepressant treatment.
5. Amygdala response to exposure to negative emotional faces (fear and anger) and cerebral 5-HT4R binding is expected to be associated, both in cross-sectional comparison before treatment and with regard to longitudinal changes (changes from baseline).
6. In resting state fMRI (rsfMRI), we expect that changes from baseline in a functional network centered around dorsomedial prefrontal cortex and other networks centered around anterior cingulate cortex and posterior cingulate cortex (default mode network) respectively will predict treatment outcome.
7. Low activity in a brain network engaged in reward processing (as assessed by fMRI) will predict antidepressant treatment response (i.e. positive association between low activity and remitter- relative to non-responder group) both in cross-sectional comparison before treatment and with regard to longitudinal changes (changes from baseline).
8. We expect reduced evoked gamma-activity measured with EEG and "Event-related-potentials" (ERP) in MDD patients compared to controls.
9. Using EEG/ERP, we expect reduced alpha and theta cordance and higher theta band activity among remitters measured at baseline.
10. Higher levels of systemic inflammation will predict poor cognitive function at baseline, and a poor antidepressant treatment outcome.
11. A blunted cortisol awakening response (CAR) at baseline will predict a positive antidepressant treatment outcome.
12. Restored HPA-axis dynamics (increased CAR compared to baseline) at week 8 will be associated with a positive treatment outcome.
13. There will be a significant association between functional networks found by EEG and fMRI, both at baseline and as contrast between remitters and non-responders after 8 weeks.
14. Hippocampal volume will predict treatment outcome.
15. Patients with a low 5-HT4R binding (as imaged by 11C-SB207145 PET) at baseline before treatment will have a lower libido relative to patients with a high binding.
16. Patients who respond to antidepressant treatment with the most pronounced changes in 5-HT4R binding (as imaged by 11C-SB207145) will experience a larger degree of sexual side-effects; i.e., the magnitude of 5-HT4R binding changes from baseline will be positively associated with the degree of sexual side-effects.
17. Childhood abuse (defined by a composite measure of early life stress questionnaires) and anxious depression (defined as anxiety/somatization factor score in HAMD-17 > 7) predicts poor antidepressant treatment outcome.
18. We expect urinary markers of oxidatively generated DNA/RNA damage to increase more in responders than in non-responders.
19. With an exploratory approach it will be possible to identify a set of predictors of antidepressant treatment outcome. This explorative analysis includes genetics, epigenetics, peripheral and central neurobiological characteristics, neuropsychology testing outcomes, psychometry including self-reported mental distress, both cross-sectional at baseline and as longitudinal measures.

Ethical Aspects:

The study protocol complies with the Declaration of Helsinki II and approval by all relevant authorities will be obtained before initiation. All human volunteers will receive oral and written information about the given study and provide written informed consent before enrolment. The trial is monitored by a Good Clinical Practise unit for the relevant domains.

• Study Type: Interventional
• Enrollment (Anticipated): 100
• Phase: Phase 1

Contacts and Locations

Study Locations

• Denmark
  • Copenhagen, Denmark, 2100
    • Neurobiology Research Unit, Rigshospitalet

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 16 years to 63 years (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Moderate to severe depression
• Age 18-65 years
• No previous antidepressant treatment the last 2 months
• Informed and signed consent

Exclusion Criteria:

• More than one previous attempt with antidepressant drugs
• Duration of current depression more than 2 years
• Current or previous psychiatric severe co-morbidity
• Acute suicidal ideation
• Psychotic
• Previous non-response to Selective Serotonin Reuptake Inhibitor (SSRI)
• Contraindication for SSRI treatment
• More suitable with treatment of alternative anti-depressive drug.
• Severe somatic co-morbidity
• Somatic medicine that can influence the trial
• Contraindications for MR-scanning
• Previous exposure to radioactivity > 10 milli sievert (mSv) within the last year
• Alcohol or drug abuse
• Previous severe head trauma
• Pregnancy
• Breast-feeding
• Insufficient Danish skills

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Other
• Allocation: Non-Randomized
• Interventional Model: Single Group Assignment
• Masking: None (Open Label)

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Treatment of MDD patients; Treatment of MDD patients with escitalopram	Drug: Escitalopram; Patients will be treated with an antidepressant drug (escitalopram) at flexible standard doses for 12 weeks. If no response after 4 weeks; shift to duloxetine arm.
No Intervention: Healthy controls; No treatment.
Experimental: Shift of treatment for MDD patients; Treatment of MDD patients with duloxetine	Drug: Duloxetine; Patients who at 4 weeks of escitalopram have not responded will be shifted to duloxetine at flexible standard dosages.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Binary treatment outcome in terms of remission from depression.	Treatment outcome defined as changes in HAMD-6 score after antidepressant treatment (remitters and non-responders as previously defined).	Baseline to clinical follow-up at 8 weeks after antidepressant treatment.
Baseline cerebral 5-HT4R binding as imaged by 11C-SB207145 PET.	Latent variable construct of 5-HT4R level based on quantification of 5-HT4R binding in primary volumes of interest; neocortex, nucleus caudatus, putamen and hippocampus. Assessed in depressed patients and healthy controls.	Baseline.
Changes from baseline in cerebral 5-HT4R binding as imaged by 11C-SB207145 PET	Difference in latent variable construct of 5-HT4R level based on quantification of 5-HT4R binding in primary volumes of interest; neocortex, nucleus caudatus, putamen and hippocampus. Measured in remitters and non-responders.	Baseline to follow-up scan at 8 weeks after antidepressant treatment.
Baseline hippocampus volume	Structural MRI scan in depressed patients and healthy controls.	Baseline.
Changes from baseline in hippocampus volume.	Structural MRI in remitters and non-responders.	Baseline to follow-up scan at 8 weeks after antidepressant treatment.
Baseline fMRI BOLD response to an emotional faces paradigm	fMRI (BOLD response) based assessment of brain activity to emotionally salient, relative to neutral, stimuli.	Baseline
Changes from baseline in fMRI BOLD response to an emotional faces paradigm	fMRI (BOLD response) based assessment of brain activity to emotionally salient, relative to neutral, stimuli.	Baseline to follow-up scan at 8 weeks after antidepressant treatment.
Baseline fMRI BOLD response to reward paradigm.	fMRI (BOLD response) based assessment of brain activity in response to reward, relative to non-reward, stimuli.	Baseline
Changes from baseline in fMRI BOLD response to reward paradigm	fMRI (BOLD response) based assessment of brain activity in response to reward, relative to non-reward, stimuli.	Baseline to follow-up scan at 8 weeks after antidepressant treatment.
Baseline rsfMRI based spontaneous co-fluctuations in low frequency BOLD signal (functional connectivity)	Assessed with rsfMRI scan in the resting state, i.e. non-goal oriented spontaneous thought and awake.	Baseline
Changes from baseline in rsfMRI spontaneous co-fluctuations in low frequency BOLD signal (functional connectivity)	Assessed with rsfMRI scan in the resting state, i.e. non-goal oriented spontaneous thought and awake.	Baseline to follow-up scan at 8 weeks after antidepressant treatment.
Sexual function in depression	Assessed with scores of self reported sexual function questionnaires in depressed patients and healthy controls.	Baseline
Changes in sexual function	Questionnaire-based self-reported sexual function in remitters and non-responders	Baseline to clinical follow-up at 8 or 12 weeks after antidepressant treatment, and baseline to follow-up scan at 8 weeks after antidepressant treatment.
Baseline EEG including event related potentials (ERP)	Assessment of evoked gamma activity, alpha and theta cordance band activity in depressed patients and healthy controls.	Baseline
Changes in EEG including event related potentials (ERP)	Assessment of evoked gamma activity, alpha and theta cordance band activity in remitters and non-responders.	Baseline to follow-up examination at 8 weeks after antidepressant treatment.
Cortisol awakening response	Cortisol changes in response to awakening as measured in saliva from 0 to 60 minutes after awakening in depressed patients and healthy controls.	Baseline
Changes in cortisol awakening response (HPA-axis dynamics)	Measured in remitters and non-responders.	Baseline and follow-up examination at 8 weeks after antidepressant treatment.
Systemic inflammation peripheral blood hsCRP and immunoactive cytokines	Measured with peripheral blood markers in plasma by high-sensitivity (hs) methods.	Baseline and follow-up examination at 8 weeks after antidepressant treatment.
Changes in systemic inflammation peripheral blood hsCRP and immunoactive cytokines	Measured with peripheral blood markers in plasma by high-sensitivity (hs) methods.	Baseline and follow-up examination at 8 weeks after antidepressant treatment.
Systemic oxidative stress in terms of 8-oxodG and 8-oxoGuo in urine	8-oxodG and 8-oxoGuo measured with mass spectrometry in spot-urine and normalized to urinary creatinine, in depressed patients and healthy controls.	Baseline
Changes in systemic oxidative stress in terms of 8-oxodG and 8-oxoGuo in urine	8-oxodG and 8-oxoGuo measured with mass spectrometry in spot-urine and normalized to urinary creatinine, in remitters and non-responders.	Baseline and follow-up examinations at 8 weeks after antidepressant treatment.
Early life Stress	Self-reported early life stress with the Children Abuse and Trauma Scale (CATS) questionnaire.	Baseline
Performance on Verbal Affective Memory Tasks (VAMT-26).	Differences between healthy controls and depressed patients at baseline and week 12 follow-up, as well as longitudinal alterations to treatment response in MDD patients.	From baseline to follow-up after 12 weeks of treatment with antidepressant treatment.
Performance on Moral Judgement Task	Differences between healthy controls and depressed patients at baseline and week 12 follow-up, as well as longitudinal alterations to treatment response in MDD patients.	From baseline to follow-up after 12 weeks of treatment with antidepressant treatment.
Performance on Letter-Number Sequence Task.	Differences between healthy controls and depressed patients at baseline and week 12 follow-up, as well as longitudinal alterations to treatment response in MDD patients.	From baseline to follow-up after 12 weeks of treatment with antidepressant treatment.

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Changes from baseline in HAMD-6 score	Score indexing changes in severity of the depressed state	Baseline to follow-up at 8 and 12 weeks
HAMD-6 score after 8 and 12 weeks of antidepressant treatment	Score indexing severity of the depressed state	Week 8 and 12 of treatment period
Regional 5-HT4R binding	Measurement of 5-HT4R binding in (a) striatum (caudate nuclei and putamen), (b) a pooled limbic region (amygdala, hippocampus, thalamus, anterior- and posterior cingulate cortex,) (c) a pooled neocortex region (parietal cortex, occipital cortex, lateral temporal cortex, insula, orbito-frontal and lateral-frontal cortex).	Measured at baseline and after 8 weeks of antidepressant treatment.
Sexual side-effects from antidepressant treatment	Perceived side effects from self reported questionnaires	8 weeks of antidepressant treatment
Baseline latent variable construct of self-reported mental state	Composed by latent variable structural equation modelling of self-reported mental state from questionnaires score of: Becks Depression Inventory -II (BDI-II), Perceived Stress Scale (PSS), Snaith-Hamilton Pleasure Scale (SHAPS), Rumination Response Scale (RRS), Pittsburgh Sleep Quality Index (PSQI), Generalized Anxiety Distress Assessment 10 item (GAD-10), Activity level, Profile of Mood States (POMS), Visual Analogue Scale for mental distress (VAS) and Brief symptom Inventory-53 item (BSI-53)) in depressed patients and healthy controls.	Baseline
Baseline self reported family history of mood disorders	Family History Assessment Module (OS-FHAM) in depressed patients and healthy controls.	Baseline
Changes from baseline in self-reported mental state questionnaire-based latent variable construct	Composed by latent variable structural equation modelling of changes from baseline in self-reported mental state from questionnaires score of: Becks Depression Inventory -II (BDI-II), Perceived Stress Scale (PSS), Snaith-Hamilton Pleasure Scale (SHAPS), Rumination Response Scale (RRS), Pittsburgh Sleep Quality Index (PSQI), Generalized Anxiety Distress Assessment 10 item (GAD-10), Activity level, Profile of Mood States (POMS) and Brief symptom Inventory-53 item (BSI-53) in remitters and non-responders.	At baseline and repeated across the study period to last follow-up after 12 weeks of antidepressant treatment.
Total daily cortisol output	Area under curve of 8 serial measures of salivary cortisol concentrations during an assessment day	Baseline (before treatment)
Changes in total daily cortisol output	Difference in area under curve of 8 serial measures of salivary cortisol concentrations during an assessment day	Baseline (before treatment) to 8 weeks of antidepressant treatment
Parental bonding quality	Self-reported parental bonding quality as assessed in baseline by parental bonding interview (PBI)	Baseline
5-HTTLPR genotype status	5-HTTLPR genotype status (binary), i.e. high-expressing LALA vs low-expressing (S or LG) variants	Baseline
Epigenetic FK506-binding protein 51 (FKBP5) status at baseline	Methylation of the FKBP5 gene	Baseline
Changes in epigenetic FKBP5 status from baseline	Changes in methylation status of the FKBP5 gene	Baseline to 8 and 12 weeks of intervention
Epigenetic 5-HTTLPR status at baseline	Methylation status of the 5-HTTLPR gene	Baseline
Changes in epigenetic 5-HTTLPR status from baseline	Changes in methylation status of the 5-HTTLPR gene	Baseline to 8 and 12 weeks of intervention
Epigenetic spindle and kinetochore associated complex subunit 2 (SKA2) status at baseline	Methylation status of the SKA2 gene	Baseline to 8 and 12 weeks of intervention
Changes in epigenetic SKA2 status from baseline	Changes in methylation status of the SKA2 gene	Baseline to 8 and 12 weeks of intervention
Performance on Face and Eyes Emotion Recognition Task	Differences between healthy controls and depressed patients at baseline and week 12 follow-up, as well as longitudinal alterations to treatment response in MDD patients.	From baseline to follow-up after 12 weeks of treatment with antidepressant treatment.
Performance on Intensity Morphing Task	Differences between healthy controls and depressed patients at baseline and week 12 follow-up, as well as longitudinal alterations to treatment response in MDD patients.	From baseline to follow-up after 12 weeks of treatment with antidepressant treatment.
Performance on Social Information Preference Task	Differences between healthy controls and depressed patients at baseline and week 12 follow-up, as well as longitudinal alterations to treatment response in MDD patients.	From baseline to follow-up after 12 weeks of treatment with antidepressant treatment.
Performance on Simple Reaction Time.	Differences between healthy controls and depressed patients at baseline and week 12 follow-up, as well as longitudinal alterations to treatment response in MDD patients.	From baseline to follow-up after 12 weeks of treatment with antidepressant treatment.

Collaborators and Investigators

• Sponsor: Rigshospitalet, Denmark
• Collaborators: Center for Integrated Molecular Brain Imaging, Copenhagen, Denmak; Psychiatric Centre Copenhagen; Central Visitation, Region Hovedstaden
• Investigators: Principal Investigator: Gitte M Knudsen, MD,Prof., Neurobiology Research Unit

Publications and helpful links

General Publications

• Ostergaard SD, Bech P, Miskowiak KW. Fewer study participants needed to demonstrate superior antidepressant efficacy when using the Hamilton melancholia subscale (HAM-D(6)) as outcome measure. J Affect Disord. 2016 Jan 15;190:842-845. doi: 10.1016/j.jad.2014.10.047. Epub 2014 Nov 7.
• Dam VH, Stenbaek DS, Kohler-Forsberg K, Cheng Ip, Ozenne B, Sahakian BJ, Knudsen GM, Jorgensen MB, Frokjaer VG. Evaluating cognitive disturbances as treatment target and predictor of antidepressant action in major depressive disorder: A NeuroPharm study. Transl Psychiatry. 2022 Nov 8;12(1):468. doi: 10.1038/s41398-022-02240-1.
• Fisher PM, Ozenne B, Ganz M, Frokjaer VG, Dam VN, Penninx BW, Sankar A, Miskowiak K, Jensen PS, Knudsen GM, Jorgensen MB. Emotional faces processing in major depressive disorder and prediction of antidepressant treatment response: A NeuroPharm study. J Psychopharmacol. 2022 May;36(5):626-636. doi: 10.1177/02698811221089035. Epub 2022 May 13.
• Ip CT, Olbrich S, Ganz M, Ozenne B, Kohler-Forsberg K, Dam VH, Beniczky S, Jorgensen MB, Frokjaer VG, Sogaard B, Christensen SR, Knudsen GM. Pretreatment qEEG biomarkers for predicting pharmacological treatment outcome in major depressive disorder: Independent validation from the NeuroPharm study. Eur Neuropsychopharmacol. 2021 Aug;49:101-112. doi: 10.1016/j.euroneuro.2021.03.024. Epub 2021 Apr 25.
• Kohler-Forsberg K, Jorgensen A, Dam VH, Stenbaek DS, Fisher PM, Ip CT, Ganz M, Poulsen HE, Giraldi A, Ozenne B, Jorgensen MB, Knudsen GM, Frokjaer VG. Predicting Treatment Outcome in Major Depressive Disorder Using Serotonin 4 Receptor PET Brain Imaging, Functional MRI, Cognitive-, EEG-Based, and Peripheral Biomarkers: A NeuroPharm Open Label Clinical Trial Protocol. Front Psychiatry. 2020 Jul 23;11:641. doi: 10.3389/fpsyt.2020.00641. eCollection 2020.

Study record dates

Study Major Dates

• Study Start: August 1, 2016
• Primary Completion (Actual): July 1, 2019
• Study Completion (Actual): July 1, 2019

Study Registration Dates

• First Submitted: July 29, 2016
• First Submitted That Met QC Criteria: August 11, 2016
• First Posted (Estimate): August 16, 2016

Study Record Updates

• Last Update Posted (Actual): October 16, 2019
• Last Update Submitted That Met QC Criteria: October 14, 2019
• Last Verified: October 1, 2019

More Information

Terms related to this study

• Keywords: MRI; Oxidative stress; Inflammation; Anxiety; PET; MDD; fMRI; EEG; Mental health; Serotonin; Psychometrics; Escitalopram; Sexual function; Brain imaging; Selective Serotonin Reuptake Inhibitor; Social cognition; ERP; 5-HT4R; Cortisol awakening response; Prediction of treatment response; 5-HTTLPR; Early life stress; Moderate depression; Severe depression; rsfMRI; 11C-SB207145; Affective cognition; Cold cognition
• Additional Relevant MeSH Terms: Behavioral Symptoms; Mental Disorders; Mood Disorders; Depression; Depressive Disorder; Depressive Disorder, Major; Physiological Effects of Drugs; Neurotransmitter Agents; Molecular Mechanisms of Pharmacological Action; Peripheral Nervous System Agents; Analgesics; Sensory System Agents; Psychotropic Drugs; Serotonin Uptake Inhibitors; Neurotransmitter Uptake Inhibitors; Membrane Transport Modulators; Serotonin Agents; Antidepressive Agents; Dopamine Agents; Antidepressive Agents, Second-Generation; Serotonin and Noradrenaline Reuptake Inhibitors; Duloxetine Hydrochloride; Citalopram
• Other Study ID Numbers: NP1

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: Yes
• IPD Plan Description: Via database of Center for Integrated Molecular Brain Imaging (Knudsen et al 2016, NeuroImage) data will be available for neuroscience research community contingent on approval by scientific board.