Intake of Beta-glucan and Postprandial Regulation of Blood Glucose Metabolism in Healthy Subjects

Beta-glucan and Blood Glucose

The overall aim is to investigate the intake of beta-glucan in relation to glucose metabolism and satiety in a postprandial study with healthy subjects. The potential effects will be related to changes in the gut microbiota, the circulating levels of short chain fatty acids, inflammation and gene expression in peripheral mononuclear blood cells

Study Overview

• Status: Completed
• Conditions: Gut Microbiota; Satiety; Post Prandial Blood Glucose
• Intervention / Treatment: Other: 0.5 g beta-glucan; Other: 3.5 g beta-glucan; Other: 8 g beta-glucan

Detailed Description

The intervention study will have a fixed order, cross-over design with three test meals containing low (0.5 g / 100 g of product), medium (3.5 g / 100 g of product) and high (8 g / 100 g product) amount of beta -glucans, respectively. The test meals are in the form of cereals. All participants will eat the three test meals three constitutive days with 2 weeks apart. At day four, the participants will perform a postprandial glucose test (OGTT, 75 g glucose in 150 ml water) at Oslo and Akershus University College. Blood samples will be taken before and at different time points after glucose test.

At the screening visit the participants will be asked to limit the intake of dietary fiber from grains two weeks prior to the baseline visit (0) and during the study. Otherwise, participants will be asked not to change their diet and exercise habits during the study period.

At the baseline visit (visit 0) an OGTT will be performed. OGTT will also be performed at visit 1, 3 and 5 after intake of low, medium and high beta-glucan, respectively.

The participants will receive the test meals at visit 1, 2 and 4.

• Study Type: Interventional
• Enrollment (Actual): 14
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Norway
  • Oslo, Norway, 0130
    • Oslo and Akershus University College

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 65 years (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• BMI between 18,5 and 27 kg/m2
• Fasting plasma glucose ≤ 6.1 mmol/l

Exclusion Criteria:

• Chronic metabolic diseases such as diabetes type 1 and 2, coronary heart disease and cancer the last 6 months.
• Intestinal diseases such as chrons disease, ulcerative colitis and irritable bowel syndrome.
• Food allergy and intolerances towards grain and dairy products.
• Pregnant and lactating
• Smokers
• Fasting blood glucose ≥ 6.1 mmol/L
• CRP > 10 mg/L, measured at baseline (visit 0)
• BMI <18,5 and >27 kg/m2
• Planned weight reduction and or ± 5% weight change over the past three months.
• Use of antibiotics last 3 months before study entry and during the study period
• Use of probiotics the last month before study entry and during the study period
• Blood donor last 2 months before study entry and or during the study period
• Not willing to end the use of dietary supplements four weeks prior to study entry and throughout the study period
• Alcohol consumption > 40g / day
• Hormone treatments (except contraceptives)

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Non-Randomized
• Interventional Model: Crossover Assignment
• Masking: Triple

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Test meal 1; Test meal with 0.5 g beta-glucan	Other: 0.5 g beta-glucan; Dietary cross-over study with beta-glucan fiber
Experimental: Test meal 2; Test meal with 3.5 g beta-glucan	Other: 3.5 g beta-glucan; Dietary cross-over study with beta-glucan fiber
Experimental: Test meal 3; Test meal with 8 g beta-glucan	Other: 8 g beta-glucan; Dietary cross-over study with beta-glucan fiber

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Postprandial blood glucose response	Blood glucose response after OGTT	At day 4
Postprandial insulin response	insulin response after OGTT	At day 4

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
H2 breath response	H2 breath response after OGTT	At day 4
Free fatty acids	Plasma free fatty acids are measured after intake of test meals	At day 4
Microbiota analyses in feces	Microbiota analyses in feces are measured before and after intake of test meals. Change in microbiota will be analysed	At day 4
Serum triglyceride response	Triglyceride response are measured are measured after OGTT	At day 4
Serum cholesterol	Serum cholesterol are measured fasting after intake of test meals	At day 4
hunger and satiety hormones (e.g. GLP1)	The response in hunger and satiety hormones after OGTT	At day 4
Inflammatory markers (e.g. CRP)	Response in Inflammatory markers are measured after OGTT	At day 4
mRNA analyses in PBMC (RT-PCR)	PBMC are collected before and after OGTT. The change in mRNA level will be analyzed	At day 4
Quantitative assessment of metabolites in urine	Morning urine are collected before and after intake of test meals. Metabolites in urine will be quantified using UHPLC-qTOF-MS. Change in metabolites before and after test meals will be analyzed	At day 4
Quantitative assessment of metabolites in plasma	Plasma for metabolome analyses are collected after intake of test meals. Metabolites in plasma will be quantified using UHPLC-qTOF-MS.	At day 4
Visual analogue scale (VAS)	Subjective hunger and satiety will be estimated after intake of test meals for one day	At day 1
Visual analogue scale (VAS)	Subjective hunger and satiety will be estimated after intake of test meals for three days	At day 3

Collaborators and Investigators

• Sponsor: Oslo Metropolitan University
• Collaborators: University of Oslo; Nofima; Mills DA
• Investigators: Principal Investigator: Mari CW Myhrstad, PhD, Oslo and Akershus University College

Publications and helpful links

General Publications

• Telle-Hansen VH, Gaundal L, Hogvard B, Ulven SM, Holven KB, Byfuglien MG, Mage I, Knutsen SH, Ballance S, Rieder A, Rud I, Myhrstad MCW. A Three-Day Intervention With Granola Containing Cereal Beta-Glucan Improves Glycemic Response and Changes the Gut Microbiota in Healthy Individuals: A Crossover Study. Front Nutr. 2022 Apr 28;9:796362. doi: 10.3389/fnut.2022.796362. eCollection 2022.

Study record dates

Study Major Dates

• Study Start: October 1, 2016
• Primary Completion (Actual): June 1, 2017
• Study Completion (Actual): June 1, 2017

Study Registration Dates

• First Submitted: March 10, 2017
• First Submitted That Met QC Criteria: September 25, 2017
• First Posted (Actual): September 26, 2017

Study Record Updates

• Last Update Posted (Actual): September 26, 2017
• Last Update Submitted That Met QC Criteria: September 25, 2017
• Last Verified: September 1, 2017

More Information

Terms related to this study

• Keywords: Gut microbiota; Satiety; Blood glucose; beta-glucan; Dietary intervention
• Other Study ID Numbers: 2016/648