- ICH GCP
- US Clinical Trials Registry
- Klinisk forsøg NCT03398889
Intestinal Microbiome and Extremes of Atherosclerosis
Studieoversigt
Status
Detaljeret beskrivelse
Patients were phenotyped by their residual score in linear multiple regression with measured carotid plaque burden as the dependent variable and coronary risk factors were predictors. The residual score essentially represents the distance off the regression line of predicted plaque. They were grouped into three categories: Unexplained atherosclerosis (with more plaque than predicted by risk factors; residual score >2); Explained (the amount of plaque predicted by risk factors, residual score >-2 and <2); and Protected (less plaque than predicted by risk factors, residual score <-2).
DNA was extracted from stool samples in the lab of Dr. Allen-Vercoe at University of Guelph. RNA makeup of the intestinal microbiome was assessed in the lab of Dr. Gregory Gloor at Western. Plasma levels of trimethylamine n-oxide, p-cresylsulfate, hippuric acid, p-cresyl glucuronide, pheny acetyl glutamine and phenyl sulfate were measured by ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry in the lab of Dr. Bradley Urquhart at Western.
Nutrient intake over the past year was calculated at the Harvard School of Public Health from the 131 item self-reported and semi-quantitative Harvard Food Frequency Questionnaire (FFQ).
Estimated glomerular filtration rate was calculated from the Chronic Kidney Disease Epidemiological (CKD-EPI) equations.
Undersøgelsestype
Tilmelding (Faktiske)
Kontakter og lokationer
Studiesteder
-
-
Ontario
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London, Ontario, Canada, N6G 2V4
- Stroke Prevention & Atherosclerosis Research Centre
-
-
Deltagelseskriterier
Berettigelseskriterier
Aldre berettiget til at studere
Tager imod sunde frivillige
Køn, der er berettiget til at studere
Prøveudtagningsmetode
Studiebefolkning
Beskrivelse
Inclusion Criteria:
- Patients attending stroke prevention clinics and a Premature Atherosclerosis Clinic at University Hospital, London, ON, Canada,
- with measurements of carotid plaque burden and the risk factors used in the linear regression model.
- Willing to consent to the protocol approved by the Ethics board
Exclusion Criteria:
- Missing data on variables used in the regression model,
Studieplan
Hvordan er undersøgelsen tilrettelagt?
Design detaljer
Kohorter og interventioner
Gruppe / kohorte |
Intervention / Behandling |
---|---|
Unexplained Atherosclerosis phenotype
Residual score in linear regression >2
|
plasma levels of metabolites
Harvard food frequency questionnaire
amplification and sequencing of 16S rRNA gene variable regions in stool samples
|
Explained Atherosclerosis phenotype
Residual score in linear regression <-2, <2
|
plasma levels of metabolites
Harvard food frequency questionnaire
amplification and sequencing of 16S rRNA gene variable regions in stool samples
|
Protected Atherosclerosis phenotype
Residual score <-2
|
plasma levels of metabolites
Harvard food frequency questionnaire
amplification and sequencing of 16S rRNA gene variable regions in stool samples
|
Hvad måler undersøgelsen?
Primære resultatmål
Resultatmål |
Foranstaltningsbeskrivelse |
Tidsramme |
---|---|---|
Plasma levels of metabolites in the three phenotypes
Tidsramme: Day 1
|
Plasma levels of trimethylamine n-oxide, p-cresylsulfate, hippuric acid, p-cresyl glucuronide, pheny acetyl glutamine and phenyl sulfate
|
Day 1
|
Sekundære resultatmål
Resultatmål |
Foranstaltningsbeskrivelse |
Tidsramme |
---|---|---|
Bacterial profile of the intestinal microbiome
Tidsramme: Day 1
|
Amplification and sequencing of 16S rRNA gene variable regions
|
Day 1
|
Effect of nutrient intake on plasma levels of metabolites
Tidsramme: Day 1
|
Nutrient analysis from food frequency questionnaire
|
Day 1
|
Effect of renal function on plasma levels of metabolites
Tidsramme: Day 1
|
eGFR from CKD-EPI equations
|
Day 1
|
Samarbejdspartnere og efterforskere
Sponsor
Datoer for undersøgelser
Studer store datoer
Studiestart (Faktiske)
Primær færdiggørelse (Faktiske)
Studieafslutning (Faktiske)
Datoer for studieregistrering
Først indsendt
Først indsendt, der opfyldte QC-kriterier
Først opslået (Faktiske)
Opdateringer af undersøgelsesjournaler
Sidste opdatering sendt (Faktiske)
Sidste opdatering indsendt, der opfyldte kvalitetskontrolkriterier
Sidst verificeret
Mere information
Begreber relateret til denne undersøgelse
Yderligere relevante MeSH-vilkår
Andre undersøgelses-id-numre
- 105166
Plan for individuelle deltagerdata (IPD)
Planlægger du at dele individuelle deltagerdata (IPD)?
IPD-planbeskrivelse
Lægemiddel- og udstyrsoplysninger, undersøgelsesdokumenter
Studerer et amerikansk FDA-reguleret lægemiddelprodukt
Studerer et amerikansk FDA-reguleret enhedsprodukt
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