Effects of Whey Protein and Collagen Supplementation

Effects of Whey Protein and Collagen Supplementation on Muscle Mass and Function During Training in Young Adults

The present project aims to investigate the effects of high-quality protein (whey protein) vs. low-quality protein (collagen) on muscle mass and function (e.g., strength and power) during a 10-wk resistance training program in young adults. It will be tested the hypothesis that leucine-matched collagen should promote similar gains on muscle function and mass compared to whey protein supplementation.

Study Overview

• Status: Completed
• Conditions: Hypertrophy; Exercise; Muscle Strength; Resistance Training; Nutrition Therapy
• Intervention / Treatment: Dietary supplement: Whey protein supplementation; Dietary supplement: Collagen supplementation

Detailed Description

The purpose of this study will be to investigate the effects of whey protein vs. leucine-matched collagen supplementation on muscle mass and function after a 10-wk resistance training program. Initially, all participants will be submitted for a 2-wk familiarization period, and then randomized into two groups (N = 11 / group): whey protein group and leucine-matched collagen group. The following variables will be assessed from pre-to post-training: anthropometric, muscle thickness from vastus lateralis and biceps brachii muscles using ultrasound, training load, mean power and peak torque for unilateral arm flexion exercise using a isokinetic dynamometer, countermovement vertical jump, nutritional intake, and testosterone-to-cortisol ratio . Shapiro-Wilk and Levine tests will be used to test the normality and homogeneity of the measurements, respectively. Two-way ANOVA (group x time) tests for repeated measures will be performed to assess changes over time and between groups for all dependent variables. Violation of sphericity will adjust using Greenhouse- Geyser correction. The Bonferroni post-hoc test will detect specific differences between groups. When appropriate, the percentage of change (∆%) will be reported according to the following equation: ∆% = [(pre-average post average) / pre-average mean] × 100. Values will be express as mean ± standard deviation. O significance level α will be 0.05.

• Study Type: Interventional
• Enrollment (Actual): 22
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Brazil
  • Paraná
    • Londrina, Paraná, Brazil, 86.041-140
      • Universidade Norte do Paraná

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 35 years (ADULT)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Men and women aged 18-35 years
• To be eutrophic (not overweight or obese)

Exclusion Criteria:

• To present a history of musculoskeletal disorders
• Be attending a systematized weight training program for at least six months
• To make use of medicines that could affect muscle function.
• To have used ergogenic supplements and anabolic steroids for at least six months before study
• To have restrictions for exercises practice considering Physical Activity Readiness Questionnaire (PAR-Q) responses.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: BASIC_SCIENCE
• Allocation: RANDOMIZED
• Interventional Model: PARALLEL
• Masking: DOUBLE

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
EXPERIMENTAL: Whey Protein; Each participant will be supplemented with whey protein (7 d/wk) during 10 weeks.	Dietary supplement: Whey protein supplementation; 35 g of whey protein (7 d/wk) during a 10-wk resistance training program (3 d/wk; 3 sets of 8-12 repetitions with an interval of 1-2 minutes between sets and exercises) involving the leg press, leg extension, and arm flexion exercises. The load will be adjusted weekly.
EXPERIMENTAL: Leucine-matched collagen; Each participant will be supplemented with collagen (7 d/wk) during 10 weeks.	Dietary supplement: Collagen supplementation; 35 g of collagen (7 d/wk) + 2 g of leucine during a 10-wk resistance training program (3 d/wk; 3 sets of 8-12 repetitions with an interval of 1-2 minutes between sets and exercises) involving the leg press, leg extension, and arm flexion exercises. The load will be adjusted weekly.

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change in biceps brachii thickness	Measured by ultrasonography pre- and post-training	Baseline and after 10 weeks
Change in muscle strength by training load	Training load for leg press, knee extension, and arm flexion exercises	Baseline, 5 and 10 weeks
Change in vertical jump	Countermovement vertical jump performance will be assessed using the Smart Jump instrument	Baseline and after 10 weeks
Change in mean power	Mean power using a isokinetic dynamometer (BIODEX)	Baseline and after 10 weeks
Change in peak torque	Peak torque using a isokinetic dynamometer (BIODEX)	Baseline and after 10 weeks
Change in vastus lateralis thickness	Measured by ultrasonography pre- and post-training	Baseline and after 10 weeks

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Change in testosterone-to-cortisol ratio	Blood samples will be collected for analyse of the testosterone-to-cortisol ratio	Baseline and after 10 weeks
Dietary intake	Dietary intake to be assessed using 3-day diet recalls.	Baseline, 2, 5, 10 weeks

Collaborators and Investigators

• Sponsor: Universidade Norte do Paraná
• Collaborators: Universidade Estadual de Londrina
• Investigators: Principal Investigator: Andreo Aguiar, Universidade Norte do Paraná

Study record dates

Study Major Dates

• Study Start (ACTUAL): September 1, 2019
• Primary Completion (ACTUAL): November 1, 2019
• Study Completion (ACTUAL): December 5, 2019

Study Registration Dates

• First Submitted: December 11, 2019
• First Submitted That Met QC Criteria: December 27, 2019
• First Posted (ACTUAL): December 30, 2019

Study Record Updates

• Last Update Posted (ACTUAL): January 30, 2020
• Last Update Submitted That Met QC Criteria: January 28, 2020
• Last Verified: January 1, 2020

More Information

Terms related to this study

• Keywords: Muscle strength; Resistance exercise; Protein supplementation; Amino acids; Muscle hypertrophy
• Additional Relevant MeSH Terms: Pathological Conditions, Anatomical; Hypertrophy
• Other Study ID Numbers: 3.058.958

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No