Antioxidant Vitaminas and Hypertrophy Adaptations to Resistance Training

Vitamins C and Vitamin E and Hypertrophy Adaptations to Resistance Traini

This investigations aims to investigate whether supplementation with antioxidant vitamins (vitamin C and vitamin E) can influence, enhancing or blocking, the hypertrophic adaptations caused by strength training in sedentary subjects.

Study Overview

• Status: Completed
• Conditions: Hypertrophy
• Intervention / Treatment: Dietary supplement: Placebo; Dietary supplement: Vitamin C and vitamin E

Detailed Description

A randomized, double-blind, placebo-control controlled trial will be conducted. In a previous session volunteers will be interviewed to determine if they meet the inclusion criteria. Will be done two weeks of flushing out other types of supplementation and strenuous physical exercise, along with familiarization with the strength training program and performance tests to be carried out during the rehearsal. Subsequently, it will be a week without exercising to start with the experimental designs. From the start of the trial, participants should avoid physical exercise. strenuous outside the training protocol and if usual, they will only be able to perform one resistance training session per week. Participants should avoid ingesting of foods that contain high amounts of antioxidant compounds: more than two juices of fruits, more than four cups of coffee or tea. Juices with high antioxidant content should be avoided. Alcoholic beverages should also be avoided.

• Group 1 (G1): Antioxidant vitamins (VitC (1000 mg) + VitE (235 mg)) + exercise
• Group 2 (G2): Placebo + exercise A supplementation period of 12 weeks will be carried out. In each period, supplementation with antioxidant vitamins: VitC (1000 mg) + VitE (235 mg) or with placebo.

All participants will ingest the supplementation between 1-3 hours before training, the days of rest in the mornings.

All participants will perform a 12-week strength training protocol. The training session performed in the sharp design will be the first training session in chronic design. Being the baseline (E0) and post-exercise (E1) assessment performed in G1, G2 and G3 also the first evaluation corresponding to the chronic design.

In the middle of the supplementation period (week 6) and at the end, two new evaluations (week 12). Baseline evaluations will be performed prior to an exercise session of week 6 (E2) and at the last exercise session (E3) of the following parameters: performance physique, body composition, micronutrient analysis, muscle ultrasound. I also know conduct post-exercise physical performance assessments Figure 2.

The strength training protocol will be carried out 4 days a week at the facilities sports of the European University. The sessions will be planned by a professional from the Sciences of Physical Activity and Sports. Sessions will be tracked through a diary.

• Study Type: Interventional
• Enrollment (Actual): 30
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Spain
  • Madrid, Spain, 28011
    • Universidad Europea de Madrid

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 30 years (ADULT)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• Experience in strength training of at least one year

Exclusion Criteria:

• Smokers
• Antioxidant supplementation
• Injuries or diseases

Study Plan

How is the study designed?

Design Details

• Primary Purpose: BASIC_SCIENCE
• Allocation: RANDOMIZED
• Interventional Model: PARALLEL
• Masking: DOUBLE

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
ACTIVE_COMPARATOR: Supplementation group; Antioxidant vitamins (Vitamin C (1000 mg) + Vitamine E (235 mg))	Dietary supplement: Vitamin C and vitamin E; 1000 mg 235 mg; Other Names: Ascorbic acid and tocopherol
PLACEBO_COMPARATOR: Placebo group; Placebo supplementation with the same aspect as supplementation	Dietary supplement: Placebo; Placebo

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
DEXA	Evaluation of muscle mass with dual energy X-ray absorptiometry	Week 0
DEXA	Evaluation of muscle mass with dual energy X-ray absorptiometry	Week 10
Anthropometry	Evaluation of muscle mass by a ISAK 1 level anthropometrist	Week 0
Anthropometry	Evaluation of muscle mass by a ISAK 1 level anthropometrist	Week 5
Anthropometry	Evaluation of muscle mass by a ISAK 1 level anthropometrist	Week 10
Bioimpedance	Evaluation of muscle mass with an Inbody	Week 0
Bioimpedance	Evaluation of muscle mass with an Inbody	Week 5
Bioimpedance	Evaluation of muscle mass with an Inbody	Week10
DOMS	Delayed onset muscle soreness evaluated with visual analoguic scale (0 no pain- 10 maximal pain)	Week 0
DOMS	Delayed onset muscle soreness evaluated with visual analoguic scale (0 no pain- 10 maximal pain)	Week 5
DOMS	Delayed onset muscle soreness evaluated with visual analoguic scale (0 no pain- 10 maximal pain)	Week 10
Manual dinamometry	Handgrip	Week 0
Manual dinamometry	Handgrip	Week 5
Leg dinamometry	Dinamometer	Week 0
Leg dinamometry	Dinamometer	Week 5
Leg dinamometry	Dinamometer	Week 10
RPE	10-point scale (no exertion 0- maximal effort 10)	Week 0
RPE	10-point scale (no exertion 0- maximal effort 10)	Week 5
RPE	10-point scale (no exertion 0- maximal effort 10)	Week 10
1 RM	Bench press and squats	Week 0
1 RM	Bench press and squats	Week 5
1 RM	Bench press and squats	Week 10
Parameters determined by ENCODER	Bench press and squats	Week 0
Parameters determined by ENCODER	Bench press and squats	Week 5
Parameters determined by ENCODER	Bench press and squats	Week 10

Collaborators and Investigators

• Sponsor: Universidad Europea de Madrid

Publications and helpful links

General Publications

• Dutra MT, Martins WR, Ribeiro ALA, Bottaro M. The Effects of Strength Training Combined with Vitamin C and E Supplementation on Skeletal Muscle Mass and Strength: A Systematic Review and Meta-Analysis. J Sports Med (Hindawi Publ Corp). 2020 Jan 8;2020:3505209. doi: 10.1155/2020/3505209. eCollection 2020.
• Dutra MT, Alex S, Mota MR, Sales NB, Brown LE, Bottaro M. Effect of strength training combined with antioxidant supplementation on muscular performance. Appl Physiol Nutr Metab. 2018 Aug;43(8):775-781. doi: 10.1139/apnm-2017-0866. Epub 2018 Jun 25.

Study record dates

Study Major Dates

• Study Start (ACTUAL): February 1, 2021
• Primary Completion (ACTUAL): April 15, 2021
• Study Completion (ACTUAL): June 30, 2021

Study Registration Dates

• First Submitted: March 11, 2021
• First Submitted That Met QC Criteria: March 30, 2021
• First Posted (ACTUAL): April 2, 2021

Study Record Updates

• Last Update Posted (ACTUAL): August 3, 2021
• Last Update Submitted That Met QC Criteria: August 1, 2021
• Last Verified: August 1, 2021

More Information

Terms related to this study

• Additional Relevant MeSH Terms: Pathological Conditions, Anatomical; Hypertrophy; Physiological Effects of Drugs; Molecular Mechanisms of Pharmacological Action; Protective Agents; Micronutrients; Antioxidants; Vitamin E; Tocopherols; Vitamins; Ascorbic Acid
• Other Study ID Numbers: CIPI/20/209

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No